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  • 1995-1999  (5)
  • 1
    Electronic Resource
    Electronic Resource
    An integrated strategy for the process development of a recombinant antibody-cytokine fusion protein expressed in BHK cells (1999)
    Springer
    Applied microbiology and biotechnology 52 (1999), S. 345-353 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Recombinant fusion proteins offer important new therapeutic approaches for the future. This report describes the use of three different genetic strategies (i.e. “mono-”, “bi-” and “tri-cistronic” vectors) to achieve stable secretion from BHK cells of a glycosylated antibody-cytokine fusion protein designed for use in anti-tumour therapy. It describes selection of a robust and effective production cell line based on stability of secretion of the product, quality of mRNA and protein products and performance in in vitro bioassays for potency. The data obtained at this stage were utilised in the selection of a suitable candidate production cell line. The relative productivity and general performance of the cells in stirred tank and fixed bed culture systems indicated that a variety of cell culture technologies provided robust tools for production of a highly selected cell clone. Consistency of the product glycosylation was determined by analysis of released oligosaccharides using matrix-assisted laser desorption ionisation – time of flight mass spectrometry and high-performance anion exchange chromatography. These investigations showed consistent expression of three glycoforms of the fusion protein which varied in their relative proportions in different culture systems and at different time points in a fixed bed reactor with continuous perfusion. In conclusion, this study dealt with a range of important scientific and technical issues which are essential for regulatory approval and commercial success of a recombinant protein and elucidates some useful markers for process development for similar recombinant biologicals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051530
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Inter- and Intra-Laboratory Testing of the Daphnia magna IQ Toxicity Test™ (1996)
    Springer
    Bulletin of environmental contamination and toxicology 57 (1996), S. 660 -666 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001289900241
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Utility maximizing bureaucrats: The bureaucrat's point of view (1995)
    Springer
    Public choice 82 (1995), S. 69-83 
    Details
    ISSN: 1573-7101
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract In this paper we develop a model to examine the extent to which bureaucrats are maximizing their own utility. In the spirit of Williamson (1964) and Simon (1957), we assume that bureaucrats are utility maximizers but not necessarily cost minimizers and we investigate how well the bureaucrat is doing in relation to his own goals. First, we hypothesize a utility function for the bureaucrat — we assume that the bureaucrat gains utility from producing municipal services as well as from hiring additional labor and capital. Then, using the concept of money metric utility, we determine the minimum expenditure necessary to achieve the same level of utility actually observed. Secondly, we determine the bureaucrat's distance from the utility maximizing choice of resources for a given level of expenditure. Finally, using a sample of public sector bureaucrats we attempt to explain the variation in wasted expenditure across municipalities and why bureaucrats might be underutilizers of each input relative to their own utility maximizing point.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01047730
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Plasma and whole blood taurine concentrations respond differently to taurine supplementation (humans) and depletion (cats) (1995)
    Springer
    European journal of nutrition 34 (1995), S. 137-142 
    Details
    ISSN: 1436-6215
    Keywords: Taurine ; plasma ; whole blood ; depletion ; supplementation ; Taurin ; Plasma ; Gesamtblut ; Taurinmangel ; Supplementation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Die vorliegende Studie untersuchte die Beziehung zwischen der Taurinkonzentration in Plasma und Gesamtblut unter normalen physiologischen Bedingungen bei Menschen und bei Katzen sowie nach oraler Taurinsupplementierung beim Menschen und nach taurinfreier Fütterung bei Katzen. Beim Menschen betrug die durchschnittliche Taurinkonzentration im Plasma 44±8 µmol/L und im Gesamtblut 222±35 µmol/L. Unter diesen „physiologisch normalen“ Bedingungen bestand zwischen der Taurinkonzentration in Plasma und Gesamtblut keine Korrelation (r=0.092). Die Plasmataurinkonzentration stieg nach oraler Taurinsupplementierung beim Menschen rasch an bzw. fiel schnell nach taurinfreier Ernährung bei Katzen ab. Die Taurinkonzentration im Gesamtblut war dagegen mehr inert und änderte sich erst nach anhaltender Taurinzufuhr beim Menschen bzw. nach andauerndem Taurinmangel bei Katzen.
    Notes: Summary In the present study the relationship between plasma and whole blood taurine was examined under normal physiological circumstances in humans and cats. In addition, the effect of taurine supplementation on plasma and whole blood taurine was evaluated in humans and the depletion of taurine pools after a taurine-deprived diet was studied in cats. The normal plasma taurine concentration in humans was 44±8 µmol/L and the whole blood taurine concentration was 227±35 µmol/L. Under normal physiological conditions plasma and whole blood taurine were not correlated (r=0.092). Plasma taurine responded rapidly to dietary supplementation in humans or the taurine depletion in cats. In contrast, the whole blood taurine pool was more inert and varied only under extremes of depletion (cats) or sustained taurine supplementation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01636947
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    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Control of Cs leachability in cementitious binders (1996)
    Springer
    Journal of materials science 15 (1996), S. 1736-1739 
    Details
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00636211
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    Paper (German National Licenses)
    Fulltext
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