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  • 1
    ISSN: 1520-5827
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1420-908X
    Keywords: Key words:P. acnes/LPS hepatitis model — Macrophages/ Kupffer cells — Tumor necrosis factor-α— Phosphodiesterase — Rolipram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Objective and Design: To study the effect of cellular cAMP-increasing agents on Propionibacterium acnes (P. acnes) and lipopolysaccharide (LPS)-induced mouse hepatitis.¶Material: Male BALB/c mice were used. Macrophages/Kupffer cells isolated from P. acnes-primed murine liver were used for the in vitro study.¶Treatment: Type IV phosphodiesterase (PDE)-specific inhibitor, rolipram, was administered (10, 30 mg/kg, p.o.). Dibutyryl cyclic AMP (dbcAMP) was injected (10, 100 mg/kg, i.p.) into the mice.¶Method: Plasma TNFα estimated by the use of an L-929 cell cytotoxic assay and plasma transaminase activities were measured for the in vivo study. The LPS-induced production of TNFα in vitro from the cultured macrophage/Kupffer cells was determined by ELISA.¶Results: Rolipram suppressed the elevation of plasma transaminases induced by injection of LPS, and dbcAMP had a tendency to suppress them. Both agents attenuated the LPS-induced release of TNFα in vivo, and suppressed the TNFα production from the cultured macrophage/Kupffer cells.¶Conclusions: These results suggest that rolipram and dbcAMP have potential to inhibit TNFa production from activated macrophage/Kupffer cells, and it may be partially involved in the protecting effect in the P. acnes/LPS hepatitis model.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1211
    Keywords: Key words MCP (CD46) ; Complement regulator ; Secretory form ; Alternative splicing ; Fertilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  A cDNA encoding a new secretory form of mouse membrane cofactor protein (MCP, CD46) was identified additionally to the membrane form cDNA. The secretory MCP, predicted from the cDNA sequence, consisted of the conserved four short consensus repeats (SCRs) plus a four amino acid-stretch. Unlike human MCP which comprises many isoforms, mouse MCP cDNA predicted a single isoform of membrane MCP with cytoplasmic tail 1 (CYT1) and serine/threonine-rich domain C (STC). To clarify the genomic origin and monomorphic alteration of these cDNAs, we cloned and analyzed a mouse genomic DNA harboring the full coding sequence of MCP from a 129/SV mouse genomic library. The mouse Mcp was a single gene ∼50 kilobases long. Eleven of the 14 coding exons of the human MCP gene and intron-exon boundary sequences were found to be conserved in the mouse gene. The STC homologue but not the STA or STB homologue in the mouse exons was functional: the latter being due to deletions and lack of consensus sequences for splicing. The sequence equivalent to cytoplasmic tail 2 (CYT2) has not been identified in the Mcp genome. Thus, the three exons (STA, STB, and probably CYT2) responsible for the polymorphism of human MCP by differential splicing were missing in the mouse Mcp gene. Unlike the case in humans, no Mcp-related genes or pseudogenes were observed in the mouse genome. The single mouse Mcp gene was mapped to the R-positive H5 band of mouse Chromosome 1 by FISH. Strikingly, one alternative exon with 73 base pairs (encoding the four new amino acids and a TGA stop codon) was discovered between the SCRIV and the STC exons; alternative splicing causes the generation of the secretory form of mouse MCP. These results on mouse MCP, together with the information concerning other mouse SCR proteins, infer that the regulator of complement activation (RCA) gene cluster is genetically diverged between humans and mice.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0942-0940
    Keywords: Keywords: Chronic subdural haematoma; postoperative recovery local coagulofibrinolysis; TPA; haemostasis.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary  Postoperative recovery of patients with chronic subdural haematoma (CSH) was investigated by comparing pre- and postoperative coagulant and fibrinolytic activity in the haematoma contents of 15 patients with SCH. Patients in this study were treated draining the haematoma cavity without irrigation, a procedure dubbed the closed drainage. Haematomas were collected during, and 24 hrs after, surgery. Postoperative fibrinolytic activity was lower than that observed pre-operatively. In particular, levels of tissue plasminogen activator activity (TPA), and fibrin and fibrinogen degradation products (FDP) all decreased. In contrast, coagulant activity increased postoperatively.  This paper will discuss the role of local coagulofibrinolysis in the postoperative recovery of CSH patients.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 78 (1995), S. 3362-3366 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Luminescence spectra and their variation with temperature have been investigated for solid films of poly(methylphenylsilane) and its branched analogs. Introduction of branching points to the polymers enhances the broad luminescence band in the visible region while suppressing the sharp UV luminescence band due to the resonant recombination of the exciton. The intensity of the visible luminescence is observed to keep on increasing with decreasing temperature in contrast with the nearly temperature-independent intensity of the resonant luminescence. The behavior is interpreted in terms of phonon-assisted tunneling between luminescence centers and nonradiative ones. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 19 (1996), S. 0 
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Two purified diets supplemented with 0 or 20 000 IU vitamin D3 kg−1 of diet were fed to amago salmon, Oncorhynchus rhodurus (Jordan & McGregor), for 22 weeks. The feeding stopped when fish with deficiency signs including reduced growth, and caudal region and fin tip damage increased in number. Deficient fish had a thinned epidermis consisting of atrophied and necrotic epidermal cells over the basal cells. In lesions of the caudal peduncle, the skin and the underlying musculature were extensively necrotized. The liver showed degenerated and necrotic hepatocytes. Respiratory epithelium and cardiac muscle fibres were swollen. Hypocalcaemia was evident. No pathological changes were found in bone, eyes, brain and alimentary tract. The control groups fed a nutritionally complete diet grew well and displayed no pathological changes. The pathological changes were reversed after 4 weeks of feeding of vitamin-D3-suppliemented diet.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 19 (1996), S. 0 
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 85 (1999), S. 4732-4734 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Hexagonal strontium-ferrite (SrM) thin films were deposited by sputtering with the target composition ratio N (Fe/Sr) in the range of 8–14. The discrepancy of the composition ratio between the films and the target was found and the deposited films were in Fe-rich. In the x-ray diffraction diagram, the observed diffraction lines varied with the composition of the target and preferential c-axis orientation can be observed in the film prepared with the target, where the composition N is 10. The grain shape as well as size was found to be independent of N. The magnetic easy axis was normal to the plane. The maximum saturation magnetization and coercivity in the perpendicular direction were about 300 emu/cc and 3.5 kOe, respectively, when the film was prepared with the target with N of 8. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 85 (1999), S. 5898-5900 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The perpendicular magnetic anisotropy (PMA) in NdFeB thin films is investigated. PMA was observed when the c axis of Nd2Fe14B phase orients perpendicular to the film plane. The c-axis orientation and hence the PMA was found to be very sensitive to composition and substrate temperature. The change of composition or substrate temperature leads to a deterioration in the c-axis orientation and PMA. However, the other parameters such as sputter power or deposition rate do not play a very important role over a wide range used in this study. Increasing the thickness of films does not cause a great deterioration of PMA up to 1500 nm, although the trend indicates that at higher values of thickness the films become isotropic. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 81 (1997), S. 4674-4676 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The magnetic properties of a SmCo layer on a Cr underlayer, which was prepared by various Ar gas pressure (PAr), were studied. A single line appeared in each X-ray diffraction diagram and its intensity became strong when PAr was decreased from 1.06 to 0.13 Pa. The microstructure observed by using atomic force microscopy showed that the surface morphological structure of Cr underlayers depended on PAr and the surface of the Cr underlayer became smoother as PAr was decreased, while the surface and the grain of the SmCo layer on the Cr underlayer were independent of PAr. The grain size of SmCo layers was as small as 30 nm with the surface roughness of about 1 nm. The coercivity, the squareness ratio, and the coercivity squareness ratio of the SmCo layer on the Cr underlayer prepared at 0.13 Pa were 155 kA/m, 0.92 and 0.92, respectively. Those values suggest that an easy axis of magnetization for the SmCo layer is in plane and the switching field distribution is very small. Although the crystal structure of the SmCo layer has not been clarified yet, it was found that the magnetic properties of SmCo layers can be controlled by the crystal structure of the Cr underlayer and SmCo/Cr bilayer films are promising materials for the ultrahigh density recording media. © 1997 American Institute of Physics.
    Type of Medium: Electronic Resource
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