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  • 1995-1999  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Molecular mimicry in diabetes mellitus: the homologous domain in coxsackie B virus protein 2C and islet autoantigen GAD65 is highly conserved in the coxsackie B-like enteroviruses and binds to the diabetes associated HLA-DR3 molecule (1998)
    Springer
    Diabetologia 41 (1998), S. 40-46 
    Details
    ISSN: 1432-0428
    Keywords: Keywords Coxsackie B virus ; peptide binding ; HLA-DR ; molecular mimicry ; IDDM.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary It has been proposed that molecular mimicry between protein 2C (p2C) of coxsackie virus B4 and the autoantigen glutamic acid decarboxylase (GAD65) plays a role in the pathogenesis of insulin-dependent diabetes mellitus (IDDM). In this study we show that the amino acid sequence of p2C which shares homology with a sequence in GAD65 (PEVKEK), is highly conserved in coxsackie virus B4 isolates as well as in different viruses of the subgroup of coxsackie B-like enteroviruses. These are the most prevalent enteroviruses and therefore exposure to the mimicry motif will be a frequent event throughout life. Presentation of the homologous peptides by HLA molecules is essential for T-cell reactivity. Therefore, we tested whether the PEVKEK motif can bind to the IDDM-associated HLA-DR1, -DR3 and -DR4 molecules. Synthetic peptides with sequences derived from p2C and GAD65 did bind to HLA-DR3 but not to HLA-DR1 or -DR4. Replacement of amino acids within the motif showed that the PEVKEK motif binds specifically to HLA-DR3. Moreover, both p2C and GAD65 peptides bind in the same position within the peptide binding groove of the DR3 molecule which is an essential requirement for T-cell cross-reactivity. The results support molecular mimicry between p2C of coxsackie B-like enteroviruses and GAD65. However, this molecular mimicry may be limited to the HLA-DR3 positive subpopulation of IDDM patients. [Diabetologia (1998) 41: 40–46]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250050864
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Epidemiological survey of an outbreak of multiresistantSerratia marcescens by PCR-fingerprinting (1995)
    Springer
    Infection 23 (1995), S. 267-271 
    Details
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Beschrieben wird der genotypische Nachweis einerSerratia marcescens-Epidemie auf einer Intensivstation mittels random-amplified polymerase chain finger-printing (RAPD-PCR). Bestimmt wurde der Genotyp von 43 multiresistentenS. marcescens-Stämmen, die zwischen Mai und November 1993 von 27 kolonisierten/infizierten Intensivpatienten isoliert wurden. Zudem wurden 43 Isolate ohne Bezug zur Epidemie typisiert. Mittels PCR-Fingerprinting wurden unter den epidemischen Isolaten 10 Genotypen identifiziert. Ein Genotyp wurde bei insgesamt 21 der 43 Isolate, bzw. 11 der 27 Patienten gefunden, inklusive bei 7 von 8 Patienten der thoraxchirurgischen Intensivstation. Dieser Genotyp wurde nicht unter den Kontrollisolaten nachgewiesen. In unserer Studie erwies sich RAPD-PCR als gut diskriminierende und reproduzierbare Methode zur epidemiologischen Aufklärung von nosokomialenS. marcescens-Ausbrüchen.
    Notes: Summary During an outbreak ofSerratia marcescens from May to November 1993 43 strains obtained from 27 ICU patients infected or colonized with multiresistantS. marcescens were genotypically characterized with random amplified polymerase chain reaction (RAPD-PCR)-fingerprinting. In addition, 43 epidemiologically unrelated control isolates were selected. PCR-fingerprinting identified ten different genotypes ofS. marcescens among the outbreak related strains. One predominant genotype was demonstrated in 21/43 isolates of 11/27 patients. A cluster of this genotype was found in seven/eight patients on the cardiosurgical ICU. The epidemiologically unrelated strains all showed different genotypes as compared to the predominant type. This survey proved RAPD-PCR to be a highly discriminatory and reproducible method for epidemiological studies ofS. marcescens strains in nosocomial outbreaks.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01716283
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    Paper (German National Licenses)
    Fulltext
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