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  • 1
    ISSN: 1432-0584
    Keywords: Key words Myeloma ; Donor leukocyte infusion ; Chimerism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  A large group of patients relapsing after allogeneic bone marrow transplantation (BMT) have obtained remission after infusion of leukocytes from their original donor, suggesting a graft-versus-myeloma effect. However, side effects such as graft-versus-host disease and myelosuppression are severe, and sometimes fatal, complications of this therapeutic approach. Previously we demonstrated that patients with leukemia who lack donor hematopoiesis in relapse after BMT experience severe and lasting aplasia after infusion of donor leukocytes. In two patients – one with extramedullary and one with marrow relapse after a sex-mismatched transplantation – we analyzed hematopoietic chimerism by cell sorting and bone marrow cultures. CD34-positive cells, CD4-CD8-positive cells, committed progenitors, and LTC-IC were of donor origin, as demonstrated by two-color fluorescence in situ hybridization (FISH). Additionally, in relapse complete donor T-cell chimerism was seen. In contrast, plasma cells were of recipient origin in the patient who had a relapse in the bone marrow. Both patients were treated with infusions of donor leukocytes from their original donor. Neither patient suffered myelosuppression, and one achieved a stable complete remission.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5117
    Keywords: boreal forest ; limnology ; subarctic ; fire ; lakes ; principal components analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Physical and chemical variables were measured in 35 lakes from Wood Buffalo National Park, northern Alberta and the Northwest Territories, Canada. Of these lakes, 22 were sinkholes, situated on limestone and gypsum, five were situated on the Canadian Shield and eight were shallow 'muskeg' lakes located on calcareous shales. All of the lakes were small to moderate in size. For each of the 35 lakes, 37 environmental variables were measured. Principal component analysis (PCA) revealed that underlying geology strongly influenced limnic properties. Shield lakes were characterized by higher concentrations of A1 and Fe, and lower pH values, specific conductivities and concentrations of ions such as, Ca, SO4, Li, Mg and Na, than either the sinkhole or the muskeg lakes. The muskeg lakes were differentiated from the sinkhole lakes by decreased Secchi depth owing to higher concentrations of dissolved organic carbon (DOC) and greater productivity, as evidenced by high concentrations of particulate organic carbon (POC) and chlorophyll a. Nitrogen (NH3 and NO2) was also notably higher at these sites. The 22 sinkhole lakes were further classified by the type of surrounding vegetation. Six vegetation groups were recognized: (1) spruce; (2) pine; (3) mixed; (4) shrubs/poplar; (5) recently burned and (6) rocky. These vegetation groups largely reflect fire history, but also differences in soils and drainage. Unlike geology, surrounding vegetation, and therefore recent fire history, generally had little influence on limnic properties. PCA showed that of the six vegetation groups, only the spruce lake group, which was characterized by high levels of DIC, was distinct.
    Type of Medium: Electronic Resource
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