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  • 1
    Electronic Resource
    Electronic Resource
    Texture-induced asymmetric reactions in TiN/Al–Cu/TiN (1995)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 78 (1995), S. 7419-7421 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Thermally induced reactions in TiN/Al–Cu/TiN have been investigated. It is observed that the amount of the reactions is different at the two interfaces between Al–Cu and TiN. While there is minimal reaction between Al–Cu and the TiN overlayer, the reaction between Al–Cu and the TiN underlayer increases the sheet resistance of Al–Cu by as much as 15%. It is further shown that the asymmetric reactions are most likely caused by the different degree of (111) texture of TiN grown on amorphous SiO2 and textured, polycrystalline Al–Cu. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.360398
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  • 2
    Electronic Resource
    Electronic Resource
    Escherichia coli-produced fish growth hormone as a feed additive to enhance the growth of juvenile black seabream (Acanthopagrus schlegeli) (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of applied ichthyology 13 (1997), S. 0 
    Details
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Recombinant growth hormone (rGH) of fish was highly efficiently synthesized by Escherichia coli cells harbouring an expression plasmid (pKLYP) containing a 600 base pairs segment of cDNA which encoded the mature region of yellowfin porgy (Acanthopagrus latus) pre-growth hormone. After dena-turation and renaturation, rGH purified from inclusion bodies became the refolding form. rGH were then processed into the fishmeal pellet at various concentrations. Juvenile black sea-bream (Acanthopagrus schlegeli) fed twice daily with meal containing 0.5% rGH exhibited significant increases (P 〈 0.01) in percentage weight gain (60%) and feed efficiency (41%) relative to fish in the control group at week 12. Growth enhancement was maintained for at least 4 weeks (at week 16) after the termination of treatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0426.1997.tb00105.x
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  • 3
    Electronic Resource
    Electronic Resource
    Optimisation of submerged arc welding process parameters in hardfacing (1996)
    Springer
    The international journal of advanced manufacturing technology 12 (1996), S. 402-406 
    Details
    ISSN: 1433-3015
    Keywords: Hardfacing ; Neural network ; Optimisation ; Simulated annealing algorithms ; Submerged arc welding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract In this paper, a feedforward neural network is used to model submerged arc welding (SAW) processes in hardfacing. The relationships between process parameters (arc current, arc voltage, welding speed, electrode protrusion, and preheat temperature) and welding performance (deposition rate, hardness, and dilution) are established, based on the neural network. A simulated annealing (SA) optimisation algorithm with a performance index is then applied to the neural network for searching the optimal process parameters. Experimental results have shown that welding performance can be enhanced by using this new approach.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01186928
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  • 4
    Electronic Resource
    Electronic Resource
    Optimization of the solubilization and renaturation of fish growth hormone produced by Escherichia coli (1997)
    Springer
    Applied microbiology and biotechnology 48 (1997), S. 66-72 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Growth hormone (GH) enhances the growth rate of aquacultured fish and shellfish, but it is difficult to extract native GH from fish pituitary glands. However, fish recombinant GH (rGH) can be efficiently synthesized by Escherichia coli cells, although it exists in denatured form in inclusion bodies (IB). We studied the solubilization of IB and the renaturation of rGH to help facilitate the production of a large amount of biologically active rGH. A 100-ml sample of rGH-producing E. coli produced 73.43 ± 5.47 mg IB (dry weight, n = 3) after 20 h induction by 1 mM isopropyl β-o-thiogalactopyranoside. Interestingly, if the bacteria were induced by 0.1 mM β-lactose, 95.3 ± 3.43 mg of IB was obtained. The optimal conditions for denaturation and renaturation of rGH were when IB were solubilized in 6 M guanidine hydrochloride and then dialysed against pH 10 dialysis buffer (50 mM ammonium bicarbonate and 2 mM EDTA) containing 100 mM l-arginine, 2 mM oxidized glutathione and 2 mM reduced glutathione for 24 h at 4 °C in a volume ratio of 3 to 500. At least 20% of the denaturated rGH in IB was renatured. Juvenile black sea bream injected with 0.05 μg/g resultant rGH once every 2 weeks exhibited significant increases (P 〈 0.05) in weight gain (84%) relative to fish in the control group over a 16-week period. This process is an economical and effective way to obtain an active form of rGH biosynthesized by a prokaryotic system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051016
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