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  • Electronic Resource  (7)
  • 1990-1994  (3)
  • 1985-1989  (4)
  • 1
    ISSN: 1432-1432
    Keywords: Pongidae ; Chromosome ; Karyotype ; Restriction enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The pattern of banding induced by five restriction enzymes in the chromosome complement of chimpanzee, gorilla, and orangutan is described and compared with that of humans. The G banding pattern induced by Hae III was the only feature common to the four species. Although hominid species show almost complete chromosomal homology, the restriction enzyme C banding pattern differed among the species studied. Hinf I did not induce banding in chimpanzee chromosomes, and Rsa I did not elicit banding in chimpanzee and orangutan chromosomes. Equivalent amounts of similar satellite DNA fractions located in homologous chromosomes from different species or in nonhomologous chromosomes from the same species showed different banding patterns with identical restriction enzymes. The great variability in frequency of restriction sites observed between homologous chromosome regions may have resulted from the divergence of primordial sequences changing the frequency of restriction sites for each species and for each chromosomal pair. A total of 30 patterns of banding were found informative for analysis of the hominid geneaalogical tree. Using the principle of maximum parsimony, our data support a branching order in which the chimpanzee is more closely related to the gorilla than to the human.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1432
    Keywords: Zfy-Zfx genes ; Gene evolution ; Oryzomyne-akodontine rodents
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Zinc finger-Y (Zfy) and zinc finger-X (Zfx) genes were analyzed by Southern blotting in male and female specimens of 10 species belonging to the oryzomyne-akodontine stock of Cricetidae rodents. DNA fragments were used as characters to construct a parsimony tree of the genes. Zfx and Zfy trees in general coincide with the evolutionary history of the taxa. Both trees show Oryzomys longicaudatus genes as the outgroup whereas Akodon xanthorrhinus genes are also distant from those of the other species. Oxymycterus rufus and Bolomys obscurus share related sequences, while genes from the other six Akodon species form a group of their own. It was found that 9 out of the 10 species analyzed show Zfy amplification in a range varying from 2 to 24 copies and with a pattern that is clade specific. The estimation of the average changes per character strongly suggests that Zfy has evolved more rapidly than Zfx; our estimates of the rate of nucleotide substitution are 4.6 times higher for Zfy than for Zfx.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1572-9486
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 42 (1986), S. 622-624 
    ISSN: 1420-9071
    Keywords: Karyological conservatism ; camelids ; camelids karyology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Llama, guanaco, vicuna and alpaca show similar diploid numbers, gross chromosomal morphology and homologous G, C and NOR banding patterns. This chromosomal homology is also found in the two-humped camel and very probably in the one-humped camel as well. These findings indicate that the camelid karyotype can probably be traced back to early Miocene times. This probably represents the most extreme case of chromosomal conservatism among mammals.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 26 (1991), S. 434-440 
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The fracture behaviour and toughness of modified acrylic adhesives, based on mixtures of various acrylic monomers and rubbers polymerized by a radical mechanism have been investigated. The influence of the matrix and rubber composition on bulk morphology and mechanical properties is analysed by means of transmission and scanning electron microscopy, and dynamic-mechanical measurements. Both microtexture of the material and resin-rubber compatibility have been found to significantly influence the fracture behaviour in tension-impact tests.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-6857
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The DNA composition and the in situ hybridization of satellite fractions were analysed in the New World camelids llama, alpaca, guanaco and vicuña. In the four camelid forms, it was possible to identify a similar main band DNA and five satellite fractions (I–V) with G+C base contents ranging from 32% to 66%. Satellites II–V from llama were in situ reannealed on chromosomes from the four camelid forms. The results obtained were: (a) the four satellites hybridized with regions of C-banding (centromeric regions of all chromosomes and short arms of some autosomes); (b) in general, homologous hybridizations (llama DNA versus llama chromosomes) were more efficient than heterologous reassociations; there were however three exceptions to this rule (vicuña and alpaca satellite fraction II, chromosome group B; vicuña fraction V, chromosome groups A and B); (c) X chromosomes from the four camelids had satellites III–V but lacked satellite II, (d) no satellite fraction was detected on chromosome Y. The analysis of the in situ hybridization patterns allowed to conclude that most or all C-banded chromosome regions comprise several satellite DNA fractions. It is, moreover, proposed that there is an ample interspecies variation in the number of chromosomes that cross-react with a given satellite fraction. Our data give further support to the close genomic kinship of New World camelids.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-6857
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The karyotype of moose (2n=68) is characterized by very large C-bands close to the centromeres of most chromosomes. The C-banded material represents 40% of the genome. For further characterization of the heterochromatin chromosome spreads were treated with restriction endonucleases and the restriction enzyme (Re) banding pattern was analysed. HaeIII, AluI, MboI, RsaI and HinfI produced informative Re-bands. DdeI induced an even digestion with no banding. Staining with chromomycin A3 produced bright fluorescence in regions corresponding to C-bands. Labeling with BrdUrd during late S phase differentiates four regions in the C banded area. The sequence of these regions from centromere to telomere are: late, early, late and early replicating. The authors propose the existence of five satellite DNA families with distinctive characteristics of G-C and A-T richness and different replication timing, and point out the different clusters for the endonucleases detailed above and their varying location in the chromosomes examined.
    Type of Medium: Electronic Resource
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