ISSN:
0138-4988
Keywords:
Life Sciences
;
Life Sciences (general)
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Lactic acid and short-chain carboxylic acids C1-C5 were analyzed on an Aminex HPX-87H column using UV and RI detection. The HPLC method was applied to complex fermentation media of a microbial lactic acid formation process. The resulting chromatograms of the yeast extract or culture medium showed some UV-absorbing substances that interfered partially with peaks of short-chain carboxylic acids.In contrast to UV monitoring, RI detection gave only a few small peaks of these so-called “background chromatograms”. Also glucose used as substrate could be quantified in the culture medium, because of which RI detection should be preferred.Furthermore, a self-prepared cation-exchange resin (SAC = S-DVB) based on a poly(styrenedivinylbenzene) partially comparable in its properties with Aminex resins was useful for fast (about 4 minutes) and not too expensive determinations of lactic and acetic acid in fermentation media.Applying this separattion system to fermentation solutions detected by RI as well as UV monitoring results in the quantitative analysis of lactic acid kinetics identical with an Aminex HPX-87H column were achieved.Finally, the simplicity of lactic-acid analysis is illustrated by examples of dairy products only centrifugated and diluted in double distilled water before injecting on a cation-exchange column.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/abio.370100516
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