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  • 1
    Electronic Resource
    Electronic Resource
    Structure solution and molecular dynamics refinement of the yeast Cu,Zn enzyme superoxide dismutase (1991)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 47 (1991), S. 918-927 
    Details
    ISSN: 1600-5740
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0108768191004949
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  • 2
    Electronic Resource
    Electronic Resource
    High resolution detection of uncoated metaphase chromosomes by means of field emission scanning electron microscopy (1994)
    Springer
    Chromosoma 103 (1994), S. 393-400 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract HeLa metaphase chromosomes were exammed by means of “in lens” field emission scanning electron microscopy, which permits high resolution detection of uncoated biological samples. By using uncoated chromosomes as a model for comparison we report evidence of how traditional scanning electron microscopy techniques such as metal coating and conductive methods can generate errors in chromosome structure evaluation, since both give rise to morphological artifacts. By comparing the morphology of uncoated chromosomes obtained by two different isolation procedures, such as that utilized in standard cytogenetics and the polyamine method, we have drawn the following conclusions: (a) the standard cytogenetic method gives rise to a chromosome structure consisting of a flattened network of 10 nm fibers, in which higher order chromatin organization is absent. (b) Chromosomes obtained by the polyamine method show both three-dimensional profile and higher level folding of chromatin fibers, supporting the loop chromosome organization previously suggested by scanning electron microscopy observation of hexylene glycol isolated chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00362283
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    High resolution detection of uncoated metaphase chromosomes by means of field emission scanning electron microscopy (1994)
    Springer
    Chromosoma 103 (1994), S. 393-400 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. HeLa metaphase chromosomes were examined by means of “in lens” field emission scanning electron microscopy, which permits high resolution detection of uncoated biological samples. By using uncoated chromosomes as a model for comparison we report evidence of how traditional scanning electron microscopy techniques such as metal coating and conductive methods can generate errors in chromosome structure evaluation, since both give rise to morphological artifacts. By comparing the morphology of uncoated chromosomes obtained by two different isolation procedures, such as that utilized in standard cytogenetics and the polyamine method, we have drawn the following conclusions: (a) the standard cytogenetic method gives rise to a chromosome structure consisting of a flattened network of 10 nm fibers, in which higher order chromatin organization is absent. (b) Chromosomes obtained by the polyamine method show both three-dimensional profile and higher level folding of chromatin fibers, supporting the loop chromosome organization previously suggested by scanning electron microscopy observation of hexylene glycol isolated chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00362283
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    DNA synthesis progression in 3T3 synchronized fibroblasts: a high resolution approach (1992)
    Springer
    Histochemistry and cell biology 97 (1992), S. 181-187 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In order to investigate at ultrastructural level the mechanism of DNA synthesis progression during the different moments of S-phase a bromodeoxyuridine-anti bromodeoxyuridine (BrdU-anti BrdU) method has been applied to synchronized 3T3 fibroblasts. After 30 min BrdU incorporation, five different labelling patterns can be identified and should be related to early, middle and late S-phase. These patterns are represented mainly by diffuse labelling localized in different nuclear domains and by quite rare cases in which the labelling is limited to isolated clusters of gold particles. After a 5-min pulse with BrdU it is possible to observe isolated clusters of gold particles at each moment of S-phase, which, however, exhibit the same distribution of the five principal labelling patterns observed after 30 min incorporation. In both cases labelling can be detected in the interchromatin regions during early S-phase, at the boundary between interchromatin and heterochromatin during middle S-phase and in the heterochromatin domains during late S-phase. Considering their size, the isolated spots of labelling could be interpreted as single replication units which are subsequently activated throughout the different moments of the S-phase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267309
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  • 5
    Electronic Resource
    Electronic Resource
    In vivo transcriptional pattern in theinfC operon ofBacillus stearotbermophilus (1991)
    Springer
    Molecular genetics and genomics 227 (1991), S. 60-64 
    Details
    ISSN: 1617-4623
    Keywords: Translational initiation ; AUU initiation triplet ; Gene regulation ; Transcriptional signals ; Expression of thermophilic proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary By Northern blot and primer extension analyses it was shown that inBacillus stearothermophilus the genesinfC, rpmI andrplT constitute a single transcriptional unit; the promoter and the transcriptional start-point used in vivo were identified and the half-life of the transcript (1.2 min) was determined. No indication of multiple initiation sites nor of differential stability of different regions of the transcript was found. The results suggest thatEscherichia coli andB. stearothermophilus have a different pattern of transcription around theinfC gene cluster and that differential translational efficiency within theinfC-rpmI-rplT transcriptional unit accounts for the different levels of 1173, L35 and L20 expression. The rare AUU initiation codon is the only strictly conserved element of the several peculiar transcriptional and translational features found inE. coli infC. Upon changing this codon to AUG, we found a ca. 30-fold increased expression ofB. stearothermophilus infC, which is similar to that previously found withE. coli infC (i.e. 40-fold), and provided evidence that regulation ofinfC expression through its rare AUU initiation codon might be a general phenomenon in bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260707
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  • 6
    Electronic Resource
    Electronic Resource
    III. Yeast sequencing reports. Nucleotide sequence of 9·2 kb left of CRY1 on yeast chromosome III from strain ab972: Evidence for a ty insertion and functional analysis of open reading frame YCR28 (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 8 (1992), S. 805-812 
    Details
    ISSN: 0749-503X
    Keywords: Chromosome III ; Ty insertion ; gene disruption ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We report the 9210 bp sequence from a segment of yeat chromosome III cloned from strain AB972 in λPM3270. Analysis of this sequence and its comparison with the one derived from the corresponding segment of strain XJ24-24A revealed that the AB972 region contains a duplication of about 2 kb and a Ty element, which are not found in XJ24-24A and cause a quite significant rearrangement of the whole region. We performed analysis of YCR28, the largest open reading frame we found in both AB972 and XJ24-24A. YCR28 encodes a putative protein of 512 amino acids with some similarities to yeast allontoate permease. Its disruption does not cause any detectable phenotype on rich medium or on allantoate medium, while we observed a strain-dependent effect on senstivity to amino acid balance and to 3-aminotriazole, when cells were grown in synthetic medium.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320080915
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