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  • 1
    Electronic Resource
    Electronic Resource
    Immunohistochemical demonstration of a new thiamine diphosphate-binding protein in the rat digestive tract (1992)
    Springer
    Histochemistry and cell biology 97 (1992), S. 121-124 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We purified a new thiamine diphosphate-binding protein (ThDP-BP), produced an antiserum against it, and examined its immunohistochemical distribution in the rat gastrointestinal tract using the avidinbiotin complex method. Positive staining for ThDP-BP was found in the epithelial glands of the stomach, small intestine and large intestine, and in the nuclei of hepatocytes. Measurement of the content of thiamine and its phosphate esters in extracts from the gastric and intestinal mucosa also indicated the presence of ThDP-BP in the stomach and intestinal mucosa. ThDP-BP may be useful for investigating the absorption and metabolism of the thiamine in metabolic disorders.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267301
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Control of Co1E2 plasmid replication: negative regulation of the expression of the plasmid-specified initiator protein, Rep, at a posttranscriptional step (1994)
    Springer
    Molecular genetics and genomics 244 (1994), S. 41-48 
    Details
    ISSN: 1617-4623
    Keywords: Co1E2 ; replication control ; Rep protein ; Posttranscriptional regulation ; Antisense RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The incA gene of CoIE2 is involved in the copy number control and incompatibility. Two promoters were identified around the incA gene. Transcription of the mRNA for the essential plasmid-coded initiator protein (Rep) mainly starts at a site about 140 by upstream of the initiation codon of the Rep protein. The second transcript (RNA 1) of about 115 nucleotides with two stem-and-loop structures is entirely complementary to the 5′ untranslated region of the Rep mRNA. By using translational and transcriptional fusions of the rep gene of CoIE2 and the lacZ gene of Escherichia coli, the incA gene product was shown to regulate expression of the rep gene at a posttranscriptional step. The results also suggest that the target of the incA gene product is the 5′ untranslated region of the Rep mRNA. Deletion analyses reported here show that a region(s) about 17 to 70 by upstream of the initiation codon of the Rep protein and another region inside the coding frame are important for efficient production of the Rep protein. This suggests that some additional sequence elements other than the initiation codon and the Shine-Dalgarno region and/or a secondary structure of the Rep mRNA are required for efficient production of the Rep protein. These results show that RNA I is an antisense RNA for the Rep mRNA and imply that it might regulate expression of the rep gene at the initiation step of translation by sequestering such additional sequence elements and/or by disrupting RNA secondary structure. We propose that RNA I represents the incA gene product.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00280185
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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