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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 117 (1998), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Changes in the composition of mitochondrial DNA (mtDNA) in tobacco during the processes of long-term tissue culture and regeneration were detected by DNA gel blot analyses using several mtDNA fragments as probes. The changes in mtDNA were identified by differential accumulations of mtDNA fragments in cultured cells that were hybridized with probes atp6, cob, and nad3-rpsl2. The changes detected by each probe did not appear to be linked, since they were different with each probe and in different subcultures of calli. Based on the current results and previous detailed analyses of the changes detected by atp6, a possible model that explains the alterations in mtDNA during tissue culture is presented. In this model, the changes are attributed to multiple shifts in the equilibrium that was maintained between different subgenomic mtDNA molecules.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 28 (1997), S. 0 
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The dietary effect of arachidonic acid (20:4n-6, AA) on the composition of the head and the body, and on the pigmentation of Japanese flounder, Paralichthys olivaceus (T. & S.), was studied by means of microbound diets. Increasing levels of this essential fatty acid produced higher growth and pigmentation success in juveniles after 45 days of feeding. The role played by AA in the composition of phosphatidylinositol and eicosanoid synthesis is discussed in relation to the normal function of the neural system and the production of hormones involved in the metamorphosis and pigmentation of flatfish.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: An attempt was made to measure the apparent digestibility (AD) of cholesterol and fatty acids using 5aL-cholestane as a marker (cholestane-method) using tilapia. Oreochromis niloticus, and the freshwater prawn, Macrobrachium rosenbergii, as test animals. The results were compared with those obtained by the Cr2O3-method. Casein-based test diets containing 0.3% levels of cholestane and chromic oxide as markers were prepared for determination of AD of dietary cholesterol and fatty acids. After 2 weeks of acclimation, the test animals, tilapia (average 3.0 g wet weight) and freshwater prawn (average 0.5 g wet weight) were fed the test diets for 1 week, and faecal samples were collected over 6 subsequent days. Lipids in the diets and faeces were extracted with chloroform-methanol and saponified with 10% KOH in methanol. Gas-liquid chromatography (GLC) on 1.5% OV-17 of the unsaponifiable matters afforded the quantities of 5aL-cholestane and cholesterol, whereas that on 5% Shinchrom E-71 of the saponifiable matters provided the quantities of fatty acids. Chromic oxide was determined by a wet-digestion method. In tilapia, the AD of cholesterol determined by cholestane- and Cr2O3-methods were 78.1% and 73.5%, respectively. The AD of individual fatty acids differed with the types of fatty acids, but those of fatty acids determined by the two methods in this study were similar, except for a slightly higher value of several fatty acids such as 16:1 in the cholestane-method than in the Cr2O3-method. This indicates that 5α-cholestane as well as Cr2O3 can be conventionally used as a marker for determining AD of fatty acids and cholesterol. The cholestane-method is advantageous as it permits parallel analysis of cholesterol (or fatty acids) and 5α-cholestane by GLC using small amounts of faecal sample. The cholestane-method was also useful for analysing digestibilities of cholesterol and total lipids in freshwater prawn. However, slight differences between the cholestane- and Cr2O3-methods were observed in the AD of several fatty acids such as 14:0 and 16:1.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture nutrition 2 (1996), S. 0 
    ISSN: 1365-2095
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The valine requirement of juvenile tiger shrimp, Penaeus monodon Fabricius, was determined. Shrimp postlarvae, PL20, with a mean weight of 14 mg, were randomly distributed in 36 oval 40-L capacity fibreglass tanks at 10 shrimp per tank in a flow-through seawater system and reared for 8 weeks. Postlarvae were fed amino acid test diets containing 400 g kg−1 protein with casein and gelatine as intact sources of protein. Crystalline L-amino acids were supplemented to simulate the amino acid profile of the shrimp muscle except valine. Valine was added in graded levels to obtain 7, 10, 13, 16, 19 and 22 g kg−1 of the diet or 18, 25, 33, 40, 48 and 55 g kg−1 of dietary protein. At termination of the feeding experiment, growth and survival were determined and nutritional deficiency signs noted. The relationship between weight gain and dietary valine level was analysed by the broken-line regression method to derive the valine requirement. The dietary valine requirement of Penaeus monodon postlarvae was found to be 13.5 g kg−1 of the diet or 34 g kg−1 of dietary protein. This value was lower than the level found in the shrimp tissue.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture nutrition 1 (1995), S. 0 
    ISSN: 1365-2095
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Emulsions with different content of (n-3) PUFA and vitamin A were used to enrich Artemia and examine the effect of these nutrients on pigmentation success in turbot, Scophthalmus maximus (L.). The best pigmentation rates were obtained using an overdose of vitamin A (500 000 IU L−1), but coincided with a high incidence of skeletal deformations. Higher growth and pigmentation rates were achieved by increasing the quantity of (n-3) PUFA oil in the emulsion. The use of (n-3) PUFA-deficient diet caused the highest occurrence of albinism as well as a cessation of metamorphosis.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture nutrition 3 (1997), S. 0 
    ISSN: 1365-2095
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Two feeding experiments were conducted to assess the quantitative cholesterol requirement of the juvenile prawn, Penaeus japonicus. The prawns were fed casein-based (experiment 1) and casein- or crab protein-based (experiment 2) diets with or without supplemental cholesterol. The daily increases in quantities of body cholesterol (mg kg−1 prawn day−1) at maximum growth and dietary cholesterol intake over a 40-day feeding period were determined. Prawns fed 5 g kg−1 supplemental cholesterol, regardless of protein sources, gave the highest weight gain. Body retention efficiencies of dietary cholesterol (cholesterol retained × 100/cholesterol intake) vary among prawns fed 5 g kg−1 supplemental cholesterol (diets 3, 5 and 7) but dietary cholesterol requirements expressed as mg kg−1 body weight (BW) day−1 were not significantly different (180 to 200 mg kg−1 BW day−1). Based on dietary cholesterol requirement, the optimum dietary cholesterol levels for the juvenile prawns were estimated in relation to feeding levels. When feeding levels were 3%, 5% and 7% of body weight, optimum dietary cholesterol levels were 5.0 to 6.0, 3.6 to 4.0, and 2.6 to 2.9 g kg−1 of dry diet, respectively. The present study showed the advantages of determining daily cholesterol requirement (mg kg−1 BW day−1) at maximum growth through a factorial method in determining optimum dietary cholesterol levels in P. japonicus.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1365-2095
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Diets reduced in methionine (Met) and with oxidized oil were formulated to induce visual deficiencies in Japanese flounder, Paralichthys alivaceus (T. & S.), in an attempt to link vision with pigmentation development. Even though Met-deficient diets have proved to be cataractogenic in salmonids, no cataracts were formed in flounder eye. However, both diets produced an abnormal morphology in the retinal pigment epithelium and the photoreceptor outer segments of the fish retina. The high presence of dead nuclei in the photoreceptor inner segment suggests a loss in the visual capability of fish fed these two diets, which produced lower pigmentation success than the control. A significant correlation was also found between pigmentation success and fatty acid composition (docosahexaenoic acid and arachidonic acid) of the head polar lipid fraction. The results suggest that a deficient intake of amino acids and fatty acids produces a change in retinal structure and composition, leading to reduced visual capability and suppression of the development of normal pigmentary pattern in flatfish.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 33 (1998), S. 0 
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This study was designed to demonstrate, by use of biotin-labeled hyaluronic acid binding protein (HABP) and an avidin–enzyme system, the localization of hyaluronan (HA) in periodontal tissue of beagle dogs during experimentally induced periodontitis. Experimental periodontitis was induced in the dogs by ligation of the gingival sulcus. Experimental tissue was collected at 0, 3, 7 and 21 days after ligation. HA was revealed by strong staining in the intercellular space around epithelial cells and periodontal ligament, and by light staining in the gingival connective tissue. According to the progression of periodontal tissue breakdown, HA was detected in a small number of leukocytes and monocytes, on the surface of osteoclasts, the surface of alveolar bone, thickened endothelium and in epithelial cells related to rete peg formation. Streptomyces hyaluronidase-treated specimens gave negative staining. This study suggests that HA may be associated with the inflammatory reaction in experimental periodontitis tissue.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 33 (1998), S. 0 
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This study was designed to demonstrate, by use of biotin-labeled hyaluronic acid binding protein (HABP) and an avidin-enzyme system, the localization of hyaluronan (HA) in periodontal tissue of beagle dogs during experimentally induced periodontitis. Experimental periodontitis was induced in the dogs by ligation of the gingival sulcus. Experimental tissue was collected at 0, 3, 7 and 21 days after ligation. HA was revealed by strong staining in the intercellular space around epithelial cells and periodontal ligament, and by light staining in the gingival connective tissue. According to the progression of periodontal tissue breakdown, HA was detected in a small number of leukocytes and monocytes, on the surface of osteoclasts, the surface of alveolar bone, thickened endothelium and in epithelial cells related to rete peg formation. Streptomyces hyaluronidase-treated specimens gave negative staining. This study suggests that HA may be associated with the inflammatory reaction in experimental periodontitis tissue.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Der Orthopäde 25 (1996), S. 533-541 
    ISSN: 1433-0431
    Keywords: Schlüsselwörter Zervikale komprimierende Myelopathie ; Dorsale Dekompression ; Laminoplastikverfahren ; Extensive simultane dekomprimierende Laminektomie ; Expansive z-förmige Laminoplastik ; Expansive „Open-door-Laminoplastik“ ; Key words Posterior approach ; Cervical spine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The posterior approach to the cervical spine was the only method of access to the spinal canal until the anterior approach was developed by Robinson and Smith, and Cloward. With the accumulation of experience in posterior decompression for cervical spondylotic myelopathy (CSM), successful laminectomy was guaranteed only when lordotic alignment of the cervical spine, wide and extensive laminectomy for adequate posterior shift of the spinal cord, and stability of the spine were ensured after surgery. The thick scar formation occasionally seen subsequent to postlaminectomy hematoma could lead to an unfavorable outcome. The insertion of surgical instruments, such as a Kerrison rongeur or a curette, into the spinal canal without being aware of how narrow the canal is, or uneven decompression of the spinal cord during resection of the laminae can impinge on or distort the spinal cord and result in deterioration of neurological function. Several authors have pointed out that postoperative loss of neural function is a hazard of surgical intervention. Owing to the poor results of conventional laminectomy for cervical compression myelopathy related to the problems mentioned above, Kirita developed extensive simultaneous decompression laminectomy to avoid distortion of the spinal cord by the edges of the resected laminae. Hattori devised an expansive Z-shaped laminoplasty in which the posterior wall of the spinal canal was preserved by Z-plasty of the prepared laminae. This was an attempt to prevent the invasion of scar tissue, i. e., the so-called laminectomy membrane, which was believed to be a cause of late neurological regression. He also expected that the laminae reconstructed by Z-plasty would provide support for the spine. The introduction of high-speed air-drills allowed successful development of this procedure. In 1977, Hirabayashi introduced an epoch-making laminoplasty, the expansive open-door laminoplasty. He described the advantages of this procedure as: possibility of decompressing multiple levels of the spinal cord simultaneously, better postoperative support of the neck, allowing earlier mobilization of the patients, prevention of postoperative kyphotic deformity of the cervical spine, and reduced mobility of the cervical spine postoperatively, which helps to prevent late neurological deterioration and progression of OPLL. Subsequent to the Hirabayashi laminoplasty, various modifications and supplementary procedures have been devised for further improvement of the safety and efficacy of decompression, and for improved stability of the spine. Aims, advantages and disadvantages of laminoplasty: The aims of the laminoplasty are to expand the spinal canal, to secure spinal stability and to spare the protective function of the spine. Preservation of mobility of the spine is also a goal of this procedure for multiple level involvement. Decompression can be extended along the nerve root by facetectomy. Preservation of the posterior spinal structures permits reinsertion of the nuchal muscles and the spinal ligaments after they have been totally or partially detached. This prevents kyphosis or listhesis of the cervical spine, which often develops after laminectomy, particularly in subjects below 50 years of age. Reconstructive procedures for reattaching muscles and/or ligaments to the spinous processes are added to the laminoplasty, improving the dynamic or ligamentous stability of the spine.
    Notes: Zusammenfassung Trotz zunehmender Erfahrung mit der dorsalen Dekompression bei zervikaler spondylotischer Myelopathie (ZSM) kann eine erfolgreiche Laminektomie nur unter bestimmten Umständen garantiert werden [31, 32]. Weiterhin kann das Einführen chirurgischer Instrumente in einen engen Spinalkanal oder eine ungleichmäßige Dekompression des Rückenmarks zu neurologischen Funktionseinbußen führen [1, 4, 17]. Wegen dieser Probleme hat Kirita die Methode der extensiven simultanen dekomprimierenden Laminektomie entwickelt, um so eine Irritation des Myelons durch die scharfkantigen Ecken der resezierten Laminae zu vermeiden [14, 19]. Hattori entwickelte eine expansive z-förmige Laminoplastik, bei welcher die Hinterwand des Spinalkanals durch eine Z-Plastik der ausgedünnten Laminae erhalten wird [22]. Somit sollte ein Einsprossen von Narbengewebe verhindert und zusätzliche Stabilität für die Wirbelsäule gewährleistet werden. Hirabashi stellte 1977 ein epochemachendes Verfahren, die expansive „Open-door-Laminoplastik“, vor, welche durch später eingebrachte Modifikationen weitere Sicherheit und Effizienz gewonnen hat.
    Type of Medium: Electronic Resource
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