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  • 1985-1989  (2)
  • 1960-1964  (2)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 59 (1988), S. 1866-1868 
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: Radiation losses from the main plasma of the JAERI Tokamak-60 (JT-60) were measured by a 15-channel bolometer array and those from divertor chambers in the ion and electron drift sides were each measured by two bolometers. The bolometric detector consisting of three layers (5-μm-thick gold absorber, 7-μm-thick polyimide foil, and 0.1-μm-thick gold resistor grid) was developed for this measurement. In typical diverted discharges with NB heating, the ratios of power radiated from the main and divertor chambers to the absorbed power were approximately 10% and 20%, respectively. Intense poloidal asymmetries of radiated power localized near the X point outside the torus were observed in diverted discharges. The profiles of the radiated power were reconstructed by an Abel inversion with an asymmetric term. On the other hand, a poloidally asymmetric radiation band localized near the inner wall, "marfe,'' was observed for dense plasmas in limiter discharges with neutral beam heating.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Biochemistry 2 (1963), S. 332-334 
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Each rocket was provided with the spherical Langmuir probe in the nose cone, which was split vertically into two parts at an altitude of about 60 km. to expose the ion probe to space. The probe installed in the rocket for the day-time flight was a meshed spherical cage of molybdenum wire of 0-3 ...
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 92 (1989), S. 195-199 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An in vivo 5′-bromodeoxyuridine (BrdUrd) labeled DNA probe was used for in situ DNA-RNA hybridization. BrdUrd was incorporated into plasmid DNA by inoculating E. coli with Luria-Bertani (LB) culture medium containing 500 mg/L of BrdUrd. After purification of the plasmid DNA, specific probes of the defined DNA fragments, which contained the cloned insert and short stretches of the vector DNA, were generated by restriction endonuclease. The enzymatic digestion pattern of the BrdUrd-labeled plasmid DNA was the same as that of the non-labeled one. BrdUrd was incorporated in 15%–20% of the total DNA, that is, about 80% of the thymidine was replaced by BrdUrd. Picogram amounts of the BrdUrd-labeled DNA probe itself and the target DNA were detectable on nitrocellulose filters in dot-blot spot and hybridization experiments using a peroxidase/diaminobenzidine combination. The BrdUrd-labeled DNA probe was efficiently hybridized with both single stranded DNA on nitrocellulose filters and cellular mRNA in in situ hybridization experiments. Through the reaction with BrdUrd in single stranded tails, hybridized probes were clearly detectable with fluorescent microscopy using a FITC-conjugated monoclonal anti-BrdUrd antibody. The in vivo labeling method did not require nick translation steps or in vitro DNA polymerase reactions. Sensitive, stable and efficient DNA probes were easily obtainable with this method.
    Type of Medium: Electronic Resource
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