ISSN:
1432-203X
Keywords:
Cryopreservation
;
Vitrification
;
Plant germplasm
;
Asparagus
;
Asparagus officinalis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Cultured cells and somatic embryos derived from the mesophyll tissue of asparagus (Asparagus officinalis L.) were cryopreserved by vitrification. The vitrification solution (PVS) contains (w/v) 22% glycerol, 15% ethylene glycol, 15% propylene glycol and 7% DMSO in Murashige-Skoog medium enriched with 0.5M sorbitol. After initial cryoprotection with sorbitol supplemented MS medium containing 12% ethylene glycol, cells or embryos were exposed stepwise to 85% PVS at 0°C. They were loaded into 0.5 ml transparent straws, and were then plunged directly into liquid nitrogen. After rapid warming, PVS was removed and diluted stepwise. The highest survivals of vitrified cells and embryos were about 65 and 50%, respectively. Surviving embryos developed into plantlets.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00270083
Permalink
