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  • 1
    Electronic Resource
    Electronic Resource
    Chronic Exposure of NG 108-15 Cells to Opiate Agonists Does Not Alter the Amount of the Guanine Nucleotide-Binding Proteins Gi and GO (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 53 (1989), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: We have characterized the pertussis toxin substrate in NG 108–15 cell membranes using site-specific antisera and ADP-ribosylation. Cell membranes contain two pertussis toxin-sensitive guanine nucleotide-binding protein α-subunits (Gα) whose Rf values in gel electrophoresis coincide with those of Gαo and Gαi2. The total quantity of Gi and Go im-munoreactivity amounted to 24.3 ± 2.8 pmol/mg, whereas only 1.5 ± 0.2 pmol/mg are capable of undergoing ADP-ribosylation catalyzed by pertussis toxin. Pretreatment of cells with the agonist [D-Ala2,D-Leu2]-enkephalin (DADLE) for 24 h and DADLE or morphine for 72 h did not alter the incorporation of ADP-ribose or the immunoreactive amount of Gi and Go subunits. However, pretreatment for 72 h with naloxone increased the incorporation of ADP-ribose without an apparent change in affinity or in the immunochemically determined protein levels of Gi and Go. This indicates that the process of down-regulation and desensitization of the γ-opioid receptor neither requires quantitative alterations in the levels of Gi and Go nor changes in the degree of coupling among their subunits. In contrast, chronic exposure to antagonists seems to alter the degree of precoupling between α-and β-subunits of Gi and/or Go.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1989.tb08544.x
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  • 2
    Electronic Resource
    Electronic Resource
    Serotoninergic System in Scrapie-Infected Hamsters (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 44 (1985), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Hamsters inoculated with scrapie virus show a dramatic hypersensitivity to serotoninergic drugs, developing a behavioral syndrome not unlike that obtained with pharmacologically induced lesions of the raphe nuclei. In an attempt to explain the state of hypersensitivity and to determine whether or not serotoninergic neurons were targets of the scrapie virus, pre- and postsynaptic serotoninergic sites were studied in the cerebral cortices of scrapie-infected and sham-inoculated hamsters, [3H]Imipramine binding and the uptake of endogenous 5-hydroxytryptamine (5-HT, serotonin) in synaptosomes prepared from scrapie-inoculated animals were not different from those of controls. This suggests integrity of the serotoninergic neurons in scrapie-infected hamsters. In contrast, affinity for the 5-HT1 receptor (which modulates inhibitory response) was diminished whereas that for the 5-HT2 receptor (which modulates excitatory response) was increased. This “imbalance” between the two receptors which is amplified in in vivo responses may account for the 5-HT hypersensitivity. The alteration in the affinity of the two postsynaptic 5-HT receptors supports the observation that scrapie virus alters cell plasma membranes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1985.tb12895.x
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  • 3
    Electronic Resource
    Electronic Resource
    Superparamagnetic component in the Orgueil meteorite and Mössbauer spectroscopy studies in applied magnetic fields (1986)
    [s.l.] : Nature Publishing Group
    Nature 321 (1986), S. 501-503 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The sample of the carbonaceous chondrite Orgueil was ground into a powder and a Mossbauer absorber was prepared in a plexiglass absorber holder. Mossbauer spectra were obtained at various applied magnetic fields and temperatures. Figure 1 shows a series of Mossbauer spectra of the meteorite ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/321501a0
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  • 4
    Electronic Resource
    Electronic Resource
    Direct modulation of voltage-dependent calcium channels by muscarinic activation of a pertussis toxin-sensitive G-protein in hippocampal neurons (1989)
    Springer
    Pflügers Archiv 415 (1989), S. 255-261 
    Details
    ISSN: 1432-2013
    Keywords: Ca channels ; G-proteins ; Hippocampus ; Acetylcholine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Acetylcholine (Ach) reversibly reduces the high voltage-activated (HVA) calcium (Ca) current in hippocampal neurons. Pretreatment of the cells with pertussis toxin (PTX) abolishes the Ach effect, suggesting that PTX-sensitive GTP-binding regulatory proteins (G-proteins) are involved in the signal transduction mechanism that links Ach receptor activation to inhibition of Ca channel activity. This effect is mimicked by intracellular application of the nonhydrolyzable GTP analog GTPγS. Intracellular application of purified G-proteins restored the response to Ach in PTX-treated cells. Furthermore, Ach inhibits the Ca current independently of the presence of cyclic AMP and of the protein kinase C inhibitor H-7 and neither does the Ach effect on the Ca current seem to be correlated to a transient increase in intracellular Ca. Our results suggest that activation of the α-subunit of the PTX-sensitive G-protein could directly modulate the HVA Ca channel without involving second messenger systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00370874
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  • 5
    Electronic Resource
    Electronic Resource
    Kluver-bucy syndrome in systemic lupus erythematosus (1989)
    Springer
    Journal of neurology 236 (1989), S. 55-56 
    Details
    ISSN: 1432-1459
    Keywords: Kluver-Bucy syndrome ; Systemic lupus erythematosus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The Kluver-Bucy syndrome has not been previously reported as a complication of systemic lupus erythematosus (SLE). A 48-year-old female is described who sustained several cerebral infarcts, some of which were bitemporal, due to SLE vasculopathy. She developed a complex behavioural picture consisting of global aphasia, left-side neglect, hyperorality, hypermetamorphosis and hypersexuality. She displayed appropriate emotional reaction to visually presented objects, indicating that her Kluver-Bucy syndrome could not be explained by lack of visual recognition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00314220
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  • 6
    Electronic Resource
    Electronic Resource
    Radiologic findings in the proteus syndrome (1987)
    Springer
    Pediatric radiology 17 (1987), S. 481-485 
    Details
    ISSN: 1432-1998
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The radiological findings in two patients with the Proteus syndrome are described. Features in our two cases not previously mentioned or stressed include vertebral dysplasia and enlargement (megaspondylodysplasia), bilateral genu valgum, recurrent after surgery and intraabdominal and mesenteric lipomatosis. Emergency laparotomy was performed on the first patient who had a twisted necrotic portion of mesenteric fat. Macrodactyly, skeletal muscle atrophy and subcutaneous fat accumulation in the abdominal wall were present in both. In addition the second patient was mentally retarded and had frontal bony prominence of skull. Computed tomography was used for the specific diagnosis of the lipomatous tissues in both patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02388283
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  • 7
    Electronic Resource
    Electronic Resource
    Interaction of dimeric and monomeric enkephalins with NG108-15 hybrid cells (1986)
    Springer
    Neurochemical research 11 (1986), S. 839-850 
    Details
    ISSN: 1573-6903
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The binding of the enkephalin dimer [d-Ala2, Leu5-NH-CH2-]2 (DPE2) is characterized by (1) its high affinity for receptors on NG108-15 hybrid cells, the affinity constantK=4.7×109 M−1 is up to 8-fold that of monomers (0.6 to 1.0×109 M−1), and (2) a maximal binding capacity equal to one half that of the monomers. Kinetic studies showed that DPE2 binds with a 2-fold higher rate, k1=6.3×107 M−1min−1, than monomers (2.4 to 3.8×107 M−1min−1), and dissociates at a slower rate than monomers. Dissociation of DPE2 was consistently bi- or multiphasic but increased about 12% only after 3 hr of dissociation in the presence of a large excess of unlabeled enkephalin. The dissociation kinetics of monomers varied with enkephalin and experimental conditions used. Consistent with the value for the maximal binding capacity, the kinetic studies are interpreted in support of the hypothesis that DPE2 binds by cross-linking two subunits of one receptor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00965208
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  • 8
    Electronic Resource
    Electronic Resource
    Superparamagnetism in primitive meteorites (1988)
    Springer
    Hyperfine interactions 41 (1988), S. 827-830 
    Details
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The meteorites called carbonaceous chondrites are the least altered samples we have of the material which formed our solar system. Using57Fe Mössbauer spectroscopy we show that the carbonaceous chondrite Murchison contains a compound exhibiting superparamagnetic relaxation below 80 K.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02400518
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