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  • 1985-1989  (3)
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  • 1
    Digitale Medien
    Digitale Medien
    Nasal histamine release following hyperosmolar and allergen challenge (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 44 (1989), S. 0 
    Details
    ISSN: 1398-9995
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Allergic rhinitis is characterised by symptoms of sneezing, itching of the nose with watery secretions, and nasal obstruction. We have previously shown that patients can have the diagnosis of allergic rhinitis confirmed by nasal provocation tests and assessment of nasal inspiratory peak flow (NIPF) after specific allergen or hyperosmolar challenge. We now show that histamine is released into the nasal lavage fluid in response to such challenges. Saline lavage alone results in detectable histamine levels in the order of 5 ng/ml, but in the presence of allergen (HDM) there is a significant increase in histamine release in atopies but not in control subjects. With hyperosmolar challenge, atopies showed a biphasic response in that histamine release was increased with 1.8% and 3.6% saline but returned to baseline with 5.4% and 7.2% saline, then showing a further increase with 9.0% saline. This raises the possibility of two populations of responsive mast cells. Hyperosmolar challenge leads to symptoms of nasal itch and sneezing as well as histamine release in atopics but not in controls. This suggests that hyperosmolar challenge can be used as a simple diagnostic test for allergic rhinitis and may provide a model for nasal hyper-reactivity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1398-9995.1989.tb00441.x
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  • 2
    Digitale Medien
    Digitale Medien
    Basophil sensitivity and reactivity to monoclonal anti-human IgE afterin vitro sensitization with human myeloma IgE (1987)
    Springer
    Inflammation research 20 (1987), S. 210-212 
    Details
    ISSN: 1420-908X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Leucocytes from human peripheral blood were acid eluted and sensitizedin vitro with increasing concentrations of radiolabelled myeloma IgE. This sensitization step was performed with or without 30% IgE depleted serum. After the IgE binding, cells were washed and submitted to a challenge with monoclonal anti-IgE for the determination of the cellular sensitivity and reactivity in a histamine release assay. A sample of each of the sensitized cells was analyzed for its radioactivity and the number of basophils quantified, thus allowing the determination of the mean number of IgE molecules per basophil. Raising the IgE concentrations in the sensitization procedure led to an increase of the IgE on the basophil membrane, and to a concommitant elevation of the cell sensitivity. The presence of serum during the binding of IgE onto the cells lowers slightly the binding of IgE to the basophils but decreases strongly the cellular reactivity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02074671
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  • 3
    Digitale Medien
    Digitale Medien
    Assay for heat-labile Escherichia coli enterotoxin using sandwich erythroimmunoassay (1987)
    Springer
    Medical microbiology and immunology 176 (1987), S. 89-97 
    Details
    ISSN: 1432-1831
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract We investigated the possibility of using a sheep erythrocyte-antibody conjugate as reagent in a sandwich erythroimmunoassay (SERIA) procedure to detect and titrate Escherichia coli heat-labile enterotoxin (LT) with the naked eye. In this assay, which is based on the immunological similarity between Vibrio cholerae toxin (CT) and LT, rabbit anti-CT IgG was used as immunosorbent, and sheep erythrocytes, sensitized with the rabbit anti-CT antibodies, were used as indicator. The sensitivity of the test was demonstrated by a comparative study using an enzyme-linked immunosorbent assay (ELISA). The results obtained by SERIA with 130 samples correlated well with those of a Vero cell assay and a GM1-ELISA. The test is easy, relatively cheap and as sensitive as other standard techniques; it is particularly suited for field laboratories, especially in tropical countries, and a large number of strains may be examined daily.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00200680
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