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  • 1
    Electronic Resource
    Electronic Resource
    Physiology and Molecular Biology of Phenylpropanoid Metabolism (1989)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Plant Physiology and Plant Molecular Biology 40 (1989), S. 347-369 
    Details
    ISSN: 1040-2519
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.pp.40.060189.002023
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cellular localization of nonhost resistance reactions of parsley (Petroselinum crispum) to fungal infection (1988)
    Springer
    Planta 173 (1988), S. 197-204 
    Details
    ISSN: 1432-2048
    Keywords: Callose ; Cell wall (phenolics) ; Furanocoumarin ; Petroselinum ; Phenylalanine ammonia-lyase ; Phytophthora ; S-adenosyl-L-methionine: bergaptol O-methyltransferase ; Phytoalexin (localization)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The response of parsley seedlings (Petroselinum crispum) inoculated with zoospores of the soybean-pathogenic fungus, Phytophthora megasperma f. sp. glycinea, ranged from “immunity” to “physiological susceptibility” depending on the post-inoculation environmental conditions. Typical nonhost resistance reactions, hypersensitive cell death and the formation of small local lesions, occurred under high relative humidity and 16 h illumination per day. Localized biochemical reactions were monitored using fluorescence microscopy combined with histochemical and immunohistochemical methods. The rapid accumulation of furanocoumarin phytoalexins, wall-bound phenolics and callose was detected around infection sites. Indirect antibody staining of frozen tissue sections demonstrated the local accumulation of phenylalanine ammonia-lyase, a key enzyme of general phenylpropanoid metabolism, and S-adenosyl-L-methionine: bergaptol O-methyltransferase, a specific enzyme of the furanocoumarin pathway. The results indicate that phenylpropanoid derivatives are synthesized de novo at infection sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403011
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A stable blue-light-derived signal modulates ultraviolet-light-induced activation of the chalcone-synthase gene in cultured parsley cells (1989)
    Springer
    Planta 177 (1989), S. 228-236 
    Details
    ISSN: 1432-2048
    Keywords: Blue light ; Cell culture (gene regulation) ; Chalcone synthase ; mRNA transcription ; Petroselinum (cell culture) ; Photoreceptor ; Signal transduction chain ; Transcription (regulation)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Run-off transcription assays were used to demonstrate that both the ultraviolet (UV)-B and blue-light receptors control transcription rates for chalcone-synthase mRNA in the course of light-induced flavonoid synthesis in parsley (Petroselinum crispum Miller (A.W. Hill)) cell-suspension cultures. Blue and red light alone, presumably acting via a blue-light receptor and active phytochrome (far-red absorbing form) respectively, can induce accumulation of chalcone-synthase mRNA. The extent of the response is however considerably smaller than that obtained when these wavebands are applied in combination with UV light. A preirradiation with blue light strongly increases the response to a subsequent UV pulse and this modulating effect of blue light is stable for at least 20 h. The modulating effect is abolished by a UV induction but can be reestablished by a second irradiation with blue light.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392811
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Fluence dependence of the ultraviolet-light-induced accumulation of chalcone synthase mRNA and effects of blue and far-red light in cultured parsley cells (1986)
    Springer
    Planta 169 (1986), S. 393-398 
    Details
    ISSN: 1432-2048
    Keywords: Chalcone synthase ; Fluence-response relation ; Petroselinum ; Phytochrome ; Receptor UV-B blue-light
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The fluence dependence of the time course of accumulation of chalcone synthase mRNA in ultraviolet (UV)-light-irradiated cell suspension cultures of parsley (Petroselinum crispum) and the additional effects of blue and far-red light have been investigated. Variations of the UV fluence had no detectable influence on the initial rate of increase in mRNA amount or translational activity, nor on the preceding lag period of approximately 3 h, but strongly influenced the duration of the transient increase. The effects were the same whether the fluence rate or the time of irradiation was varied to obtain a given fluence. Blue-light pretreatment of the cells resulted in increased amounts of mRNA and abolished the apparent lag period. This effect remained cryptic without the subsequent UV-light treatment. Irradiation with long-wavelength far-red light following UV-light pulses shortened the duration of the mRNA accumulation period. This effect was not altered by a preceding blue-light treatment. Thus, three photoreceptors, a UV-B receptor, a blue-light receptor and phytochrome, participate in the regulation of chalcone synthase mRNA accumulation in this system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392136
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  • 5
    Electronic Resource
    Electronic Resource
    Synthesis of characteristic proteins in nutrient-depleted cell suspension cultures of parsley (1985)
    Springer
    Planta 166 (1985), S. 194-200 
    Details
    ISSN: 1432-2048
    Keywords: Heat-shock proteins ; Petroselinum ; Starvation related proteins ; Translational control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell suspension cultures of parsley (Petroselinum crispum) exhibited an altered pattern of protein synthesis after transfer from complete growth medium to water or medium containing no macronutrients. Similar changes occurred when cultures were grown in the original medium until the nutrients were depleted. The effect was reversible upon transfer to fresh medium and was not observed during regular subculturing of the cells. While total protein synthesis decreased sharply after nutrient depletion, the synthesis of a few characteristic proteins (starvation-related proteins, STPs) increased strongly. The protein labeled at highest rates with [35S]methionine in vivo (STP 62) had an apparent molecular weight of about 62000 and a pI of about 6.3. Although its increased rate of synthesis was therefore easily detected by labeling in vivo, translation of mRNA in vitro did not give comparable results. Thus, regulatory control may be exerted mainly at the level of translation. Synthesis of STP ceased rapidly when heat shock (37° C) was applied under conditions of nutrient depletion, whereas heat-shock proteins were strongly induced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397348
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    Paper (German National Licenses)
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