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  • 1
    Electronic Resource
    Electronic Resource
    Detection and characterization of acidic compartments (vacuoles) in Chlorella vulgaris 11h cells by 31P-in vivo NMR spectroscopy and cytochemical techniques (1987)
    Springer
    Archives of microbiology 148 (1987), S. 83-87 
    Details
    ISSN: 1432-072X
    Keywords: Acidic compartments ; Chlorella vulgaris ; Chloroquine ; Compartmentation ; Inorganic phosphate ; Intracellular pH ; in vivo nuclear magnetic resonance ; Neutral red ; Unicellular algae ; Vacuoles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Acidic inorganic phosphate (Pi) pool (pH around 6) was detected besides the cytoplasmic pool in intact cells of Chlorella vulgaris 11h by 31P-in vivo nuclear magnetic resonance (NMR) spectroscopy. It was characterized as acidic compartments (vacuoles) in combination with the cytochemical technique; staining the cells with neutral red and chloroquine which are known as basic reagents specifically accumulated in acidic compartments. Under various conditions, the results obtained with the cytochemical methods were well correlated with those obtained from in vivo NMR spectra; the vacuoles were well developed in the cells at the stationary growth phase where the acidic Pi signal was detected. In contrast, cells at the logarithmic phase in which no acidic Pi signal was detected contained only smaller vesicles that accumulated these basic reagents. No acidic compartment was detected by both cytochemical technique and 31P-NMR spectroscopy when the cells were treated with NH4OH. The vacuolar pH was lowered by the anaerobic treatment of the cells in the presence of glucose, while it was not affected by the external pH during the preincubation ranging from 3 to 10. Possible vacuolar functions in unicellular algae especially with respect to intracellular pH regulation are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425353
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  • 2
    Electronic Resource
    Electronic Resource
    A nomenclature for Λ-doublet levels in rotating linear molecules (1988)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 89 (1988), S. 1749-1753 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: It is proposed that the two Λ-doublet levels of linear molecules with nonzero electronic orbital angular momentum be labeled Λ(A') and Λ(A‘), e.g., Π(A') and Π(A‘) for Π states, etc., according to the following prescription: All series of levels in which the electronic wave function at high J is symmetric with reflection of the spatial coordinates of the electrons in the plane of rotation will be designated Λ(A') for all values of J, and all those for which the electronic wave function is antisymmetric with respect to reflection will be denoted Λ(A‘). It is emphasized that this notation is meant to supplement, and not replace, the accepted spectroscopic e/f labeling and the parity quantum number. The utility of the Λ(A')/Λ(A‘) notation is that it is of most relevance in the mechanistic interpretation of reactive or photodissociative processes involving open-shell molecules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.455121
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  • 3
    Electronic Resource
    Electronic Resource
    Muon spin relaxation for Ar+NO2 systems in transverse and longitudinal magnetic fields (1986)
    Springer
    Hyperfine interactions 32 (1986), S. 807-812 
    Details
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Surface muons produced in UT-MSL were introduced into argon gas of 4.0±0.2 atm with NO2 (0–30 ppm), and muonium signals were detected in the presence of a transverse (1.7–3.4 G) and a longitudinal magnetic field (0–3.5 kG) at 295±1 K. The cross section for the transverse relaxation was (11.0±1.0)×10−16 cm2. The relaxation rates in different longitudinal magnetic fields show that the rate does not follow the conventional equation which assumes that the relaxation occurs mainly by spin-exchange interaction. Similar measurements were performed for the Mu+O2 system. These findings indicate that chemical reactions contribute to these relaxation rates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02394988
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization of the pyrenoid isolated from unicellular green algaChlamydomonas reinhardtii: Particulate form of RuBisCO protein (1988)
    Springer
    Protoplasma 144 (1988), S. 17-24 
    Details
    ISSN: 1615-6102
    Keywords: Chlamydomonas ; Pyrenoid ; Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) ; Stroma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The pyrenoid is a protein complex in the chloroplast stroma of eukaryotic algae. After the treatment with mercury chloride, pyrenoids were isolated by sucrose density gradient centrifugation from cell-wall less mutant cells, CW-15, as well as wild type cells, C-9, of unicellular green algaChlamydomonas reinhardtii. Pyrenoids were characterized as a fraction whose protein/chlorophyll ratio was very high, and also examined by Nomarski differential interference microscopy. Most of the components consisted of 55 kDa and 16 kDa polypeptides (1∶1) which were immunologically identified as the large and small subunit of RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) protein, respectively. Some minor polypeptides were also detected. Substantial amount of RuBisCO protein is present as a particulate form in the pyrenoid in addition to the soluble form in algal chloroplast stroma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01320276
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  • 5
    Electronic Resource
    Electronic Resource
    A mechanism of electron attachment to small clusters (1989)
    Springer
    The European physical journal 12 (1989), S. 291-292 
    Details
    ISSN: 1434-6079
    Keywords: 36.40 ; 34.50.H ; 34.80.D
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Van der Waals clusters of various molecules were collisionally ionized by high-Rydberg rare gas atoms and slow electrons. Negative cluster ions thus produced were detected by mass spectroscopy. The ionization mechanism were investigated by measurements of the size- and the energy-dependences of the electron attachment cross sections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01426959
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