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Digitale Medien

Assays of urea and arginase with a biocatalytic membrane electrode operating on conductimetric principle (1989)

Macholán, L. ; Filípek, J. ; Andrýs, C.

Berlin : Wiley-Blackwell

Acta Biotechnologica 9 (1989), S. 369-377

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ISSN: 0138-4988

Schlagwort(e): Life Sciences ; Life Sciences (general)

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: A bioelectrode consisting of two parallel noble metal nets and a thin layer of gel-entrapped urease between them permits to determine urea conductimetrically with a response time 3-5 min and reproducibility 5-6% above 60 micromolar concentration of urea. Differential measurement eliminates the interference due to conductive components of the real sample. Evidence has been obtained for extended application of the method by using immobilized microbial cells or tissue slice as biocatalyst in place of the immobilized purified enzyme. It is also suitable for simple kinetical assaying arginase activity in the tissue extracts.

Zusätzliches Material: 7 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/abio.370090416

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Digitale Medien

Recent progress in developing enzyme and tissue-based membrane electrodes (1987)

Macholán, L.

Berlin : Wiley-Blackwell

Acta Biotechnologica 7 (1987), S. 547-553

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Details

ISSN: 0138-4988

Schlagwort(e): Life Sciences ; Life Sciences (general)

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: The article summarizes the work of this laboratory on the design of eleven types of amperometricpenzyme electrodes mainly based on the CLARK oxygen sensor in connection with cross-linked oxidases. Special attention is paid to the sensors coupled with plant tissue slice for determining L-ascorbic acid and phenols. A “second generation” bienzyme electrode is reported which enables tow analytes forming a metabolic couple to be determined simultaneously. A principle of the first useful model of conductimetric enzyme electrode for assaying urea and arginase is also presented.

Zusätzliches Material: 4 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/abio.370070615

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