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  • 1
    Electronic Resource
    Electronic Resource
    High-accuracy oxygen polarograph for photosynthetic systems (1988)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 59 (1988), S. 486-491 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A new system is presented here, whose design is based on the electrochemical phenomena occurring on the platinum electrode that we examined before [P. Meunier and R. Popovic (unpublished)]. The architecture of the system is articulated around an IBM-PC computer which controls the polarization of the electrodes, the triggering of the flash lamp, and the acquisition of data. The potentiostat circuit and new electrode design improve the response of the electrode to a rise time of 4 ms and a decay time constant of 21 ms, without the signal being distorted by filters and without using the first derivative of the signal. This polarograph shows a 50-dB signal-to-noise ratio (0.3% error) during experiments with the motile algae Dunaliella tertiolecta. The determination of amplitudes of oxygen production is performed with third-order cubic spline interpolations, after subtraction of the current base line. The resulting accuracy and reproducibility will enable researchers to attain a higher degree of refinement in the quantification of properties of the oxygen evolving complex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1139867
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Automatic integrating fluorometer using an Apple-II and Scope-85 (1988)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 59 (1988), S. 934-936 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: The system presented here measures the fluorescence of plant samples in in vivo and in vitro automatically, calculating the principal photosynthetic parameters of the sample (evaluation of the F0 level of fluorescence, normalization of the fluorescence curve, rate of light trapped at the PSII complex, maximum quantum yield of photochemistry, average fluorescence induction time, and evaluation of the number of PSII reaction centers) as well as measuring heat dissipation kinetics. Because this system offers higher speed, more versatility, and greater accuracy (3%) than manual methods, it is a unique and valuable tool for laboratories involved in fluorescence research in the photosynthesis field. To demonstrate the use of this system and its superior performance, this article describes classic experiments carried out with the alga Dunaliella tertiolecta.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1139753
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Growth of GaInAs-InP multiquantum wells on garnet (GGG=Gd3Ga5O12) substrate by metalorganic chemical vapor deposition (1986)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 59 (1986), S. 2261-2263 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Ga0.47In0.53As-InP multiquantum wells grown by low-pressure metalorganic chemical vapor deposition on garnet (GGG=Gd3Ga5O12 with a=12.383 A(ring)) substrates are presented for the first time. The x-ray diffraction pattern shows that the orientation of the epitaxial layer is (111) while the underlying substrate orientation is (100). The photoluminescence at 77 K is due to the GaInAs layers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.336374
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Sixth Eular Workshop on Rheumatology Research (1986)
    Springer
    Clinical rheumatology 5 (1986), S. 126-161 
    Details
    ISSN: 1434-9949
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02030982
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Immunocytochemical evidence for endogenous calcitonin and parathyroid hormone in osteoblasts from the calvaria of neonatal mice (1985)
    Springer
    Cell & tissue research 240 (1985), S. 89-93 
    Details
    ISSN: 1432-0878
    Keywords: Calcitonin ; Parathyroid hormone ; Estradiol ; Estradiol receptors ; Osteoblasts ; Immunocytochemistry ; Cryo-ultramicrotomy ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunoreactivities to endogenous calcitonin, endogenous parathyroid hormone, endogenous estradiol and estradiol receptors were studied in osteoblasts from the calvaria of neonatal mice by immunocytochemistry with the use of ultrathin sections obtained by cryo-ultramicrotomy. Tissues were fixed in glutaraldehyde, postfixed in osmium tetroxide and frozen in liquid nitrogen. Estradiol and estradiol receptors could not be detected in osteoblasts, whereas calcitonin- and parathyroid hormone-like immunoreactivities were observed in this cell type. Calcitonin and parathyroid hormone had similar subcellular localizations: immunoreactivities were observed at the plasma-membrane level, in the cytoplasmic matrix, and in the nucleus. These results provide immunocytological evidence for: 1) the internalization of calcitonin and parathyroid hormone in osteoblasts; 2) a direct participation of calcitonin and parathyroid hormone in the regulation of osteoblasts; 3) the absence of estradiol receptors and estradiol in osteoblasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217561
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    Paper (German National Licenses)
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