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  • 1
    Electronic Resource
    Electronic Resource
    A new approach to the biochemical pathology of the vascular system, using time governed laminar elution and bioluminescence analyses (1987)
    Springer
    Molecular and cellular biochemistry 73 (1987), S. 55-59 
    Details
    ISSN: 1573-4919
    Keywords: bioluminescence analyses ; diabetes ; angiopathy ; endothelial cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Endothelial cells which cover the inner wall of blood vessels were extracted for bioluminescence analyses of nucleotides and enzymes. The contaminating blood was removed by heparinization and.rinsing with ammonium chloride. The content of the endothelial cells of the rat aorta was reached by time governed laminar elution, using a saponin solution to disrupt the cell membranes. Uniformity of extraction was achieved with a Hamilton programmable pump. All the analyses of the eluted fractions showed a characteristic and reproducible peak. The activities of glucose-6-phosphate dehydrogenase and adenylate kinase were significantly higher in the diabetic animals whereas the amount of nucleotides did not differ between diabetic and control rats. The laminar elution technique combined with bioluminescence assay represents a new approach to studies of biochemical alterations in the endothelial cells. The method is also useful for extraction and analyses of other surface layers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229376
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Sorbitol metabolism in retina studied in vitro (1989)
    Springer
    Graefe's archive for clinical and experimental ophthalmology 227 (1989), S. 180-183 
    Details
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A new method is described which allows study of the retinal sorbitol metabolism in vitro. Bovine retinal tissue was incubated in tissue culture medium for 24 h and after this time showed only small morphological changes. The sorbitol content doubled at a glucose level of 5.5 mM compared with freshly prepared noncultured retina. Increasing glucose concentrations led to a gradual increase of the sorbitol content. Supplementation of the culture medium with fructose likewise enhanced the retinal sorbitol accumulation. An aldose reductase inhibitor (ICI 128436; Statil) significantly decreased the sorbitol content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02169793
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Design of coupled reactions for simplification of bioluminescence analysis (1989)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 4 (1989), S. 446-453 
    Details
    ISSN: 0884-3996
    Keywords: Bioluminescence analysis ; coupled reactions ; enzymatic cycling ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Luminescence analysis may be applied to many substances by arranging a prior reaction producing a species entering the light-emitting reaction. Under favourable conditions the two consecutive reactions are carried out simultaneously as a one-step procedure. In a bioluminescence assay, luciferase stability is frequently a problem, making it desirable to develop analytical schemes where the analytical response becomes largely independent of any impaired luciferase activity. The value of maximal emission or an approached steady-state level is a convenient and usually well-defined analytical parameter. When recording this level it is important to design the participating reactions in a way that compensates for changes in luciferase activity.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bio.1170040159
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    Paper (German National Licenses)
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