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  • 1985-1989  (2)
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Material
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Cloning and DNA homology of 3-isopropylmalate dehydrogenase genes from thermophilic bacilli (1986)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 36 (1986), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The genes coding for 3-isopropylmalate dehydrogenase from the moderate thermophile Bacillus caldotenax and the extreme thermophile Bacillus caldolyticus were cloned in Escherichia coli using pBR322 as a vector. The restriction maps of the cloned fragments were determined and the small fragments carrying the enzyme gene were subcloned. DNA-DNA hybridization of DNA fragments containing the gene from mesophiles (Bacillus subtilis, E. coli and Saccharomyces cerevisiae) and thermophiles (Bacillus coagulans, B. caldotenax, B. caldolyticus and Thermus thermophilus) were examined. The DNA fragments from the thermophiles hybridized with each other, but not with the mesophile DNA fragments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1986.tb01663.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Dependence of the growth of pH-sensitive mutants of a facultatively alkalophilic Bacillus on the regulation of cytoplasmic pH (1986)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 34 (1986), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A facultatively alkalophilic Bacillus strain, YN-2000, is able to grow over a wide pH range, from 7.0–10.5, and was suggested to regulate its cytoplasmic pH by alkalinization and acidification systems at neutral and alkaline pH, respectively [9]. Two kinds of pH-sensitive mutants were isolated after treatment with N-methyl-N′-nitro-N-nitrosoguanidine. One mutant was neutral-sensitive and unable to grow below pH 8. The other was alkaline-sensitive and unable to grow above pH 9. The neutral- and alkaline-sensitive mutants were suggested to be unable to regulate cytoplasmic pH at neutral and alkaline pH, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1986.tb01403.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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