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  • 1
    Electronic Resource
    Electronic Resource
    Diploid construction by protoplast fusion in Fulvia fulva (syn. Cladosporium fulvum): genetic analysis of an imperfect fungal plant pathogen (1988)
    Springer
    Current genetics 14 (1988), S. 567-572 
    Details
    ISSN: 1432-0983
    Keywords: Fulvia fulva ; Tomato leaf mould ; Protoplast fusion ; Complementation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Auxotrophic and drug resistant mutants have been isolated in five of the seven races of Fulvia fulva (syn. Cladosporium fulvum) following UV mutagenesis. Drug resistant mutants have also been isolated by genetic transformation using vectors conferring resistance to hygromycin B and phleomycin. Protoplasts of complementing auxotrophs or mutants with different drug resistances have been fused to form diploids as demonstrated by growth characteristics and microfluorimetry. Some of the diploid fusion products remained stable but most went on to haploidise spontaneously. Using this technique it is now possible to perform both complementation and linkage analysis in an imperfect fungal plant pathogen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00434082
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi (1989)
    Springer
    Current genetics 15 (1989), S. 177-180 
    Details
    ISSN: 1432-0983
    Keywords: Reporter gene ; Filamentous fungi ; Pathogenicity ; β-glucuronidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A chimaeric β-glucuronidase (GUS) gene has been created by ligating the Aspergillus nidulans glyceraldehyde 3-phosphate dehydrogenase promoter to the coding sequence of the E. coli uidA gene. Cotransformation of this vector into A. nidulans, A. niger and the tomato pathogen Fulvia fulva (syn. Cladosporium fulvum (Cooke)) resulted in the expression of β-glucuronidase. GUS activity was detected by growth on agar media containing X-gluc and by enzyme assays of mycelial extracts. Expression of the gene in F. fulva transformants was also easily detectable during growth in plants and did not affect pathogenicity. These results form the basis for a versatile and sensitive reporter gene system for industrial and phytopathogenic filamentous fungi.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00435503
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Transformation of Fulvia fulva, a fungal pathogen of tomato, to hygromycin B resistance (1987)
    Springer
    Current genetics 12 (1987), S. 231-233 
    Details
    ISSN: 1432-0983
    Keywords: Tomato leaf mould ; Cladosporium fulvum ; Deuteromycete
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A transformation system for the tomato pathogen Fulvia fulva has been developed. Hygromycin B resistant colonies were obtained after treatment of protoplasts with a plasmid containing an E. coli hygromycin B phosphotransferase gene fused to an Aspergillus nidulans promoter. The DNA was stably integrated into the genome. The number and sites of integrations varied among transformants. The demonstration of transformation opens the way for the molecular genetic analysis of the interaction of Fulvia with tomato.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00436885
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The transformation of protoplasts of Leptosphaeria maculans to hygromycin B resistance (1988)
    Springer
    Current genetics 13 (1988), S. 327-330 
    Details
    ISSN: 1432-0983
    Keywords: Brassica ; Oil-seed rape ; Phoma lingam ; Stem canker ; Ascomycetes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Conditions are described for the efficient isolation and regeneration of protoplasts of a fungal pathogen of brassicas, Leptosphaeria maculans. Treatment of the protoplasts with DNA of the plasmid pAN7-1 (containing an E. coli hygromycin phosphotransferase gene with Aspergillus nidulans expression signals) and plating under selective conditions resulted in the formation of hygromycin 13-resistant colonies. Southern blot analysis of resistant colonies indicated that single copies of the plasmid had integrated into different sites in the genome. In twelve of the transformants analysed so far, the integration is stable through mitosis. The demonstration of efficient transformation is an essential first step in the molecular analysis of pathogenicity of this commercially important pathogen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00424427
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    Paper (German National Licenses)
    Fulltext
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