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  • 1
    Electronic Resource
    Electronic Resource
    Aplysianin-A, an antibacterial and antineoplastic glycoprotein in the albumen gland of a sea hare,Aplysia kurodai (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 1065-1067 
    Details
    ISSN: 1420-9071
    Keywords: Antibacterial factor ; antineoplastic factor ; Aplysia ; opisthobranch ; albumen gland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Aplysianin-A, an antibacterial and antineoplastic factor in the albumen gland of the sea hareAplysia kurodai, was isolated. It had a molecular weight of approximately 320 kD and consisted of subunits with a molecular weight of 85 kD. It contained 9.8% neutral sugar. Aplysianin A showed 50% inhibition ofBacillus subtilis growth at a concentration of 4 μg protein/ml and 50% lysis of murine MM46 tumor cells at 14 ng protein/ml. A partial identity of antigenic specificity of the purified specimen with an antineoplastic factor fromAplysia eggs was observed in immunodiffusion tests.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01940736
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  • 2
    Electronic Resource
    Electronic Resource
    Separation and determination of purpurea glycosides in Digitalis purpurea leaves by micro-HPLC (1987)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 10 (1987), S. 137-140 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography, Micro-HPLC ; Purpurea glycosides ; Digitalis purpurea ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A micro high-performance liquid chromatographic (micro-HPLC) method was developed for the determination of purpurea glycoside A, purpurea glycoside B, and glucogitaloxin in Digitalis purpurea leaves. The extract of dry leaf powder was applied to a Sep-Pak silica cartridge followed by preparative thin-layer chromatography prior to micro-HPLC analysis. The analysis was performed on an ODS micro column, using acetonitrile-methanol-water (21:20:45) and ultraviolet detection (220 nm). The amounts of purpurea glycoside A, purpurea glycoside B, and glucogitaloxin per 100 mg of dry leaf powder were estimated to be 52.6, 50.7, and 108.6 μg, respectively.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240100306
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    Paper (German National Licenses)
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