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  • 1
    Electronic Resource
    Electronic Resource
    The effect of chloroquine on the intralysosomal degradation of cell-coat glycoproteins in the absorptive cells of cultured human small-intestinal tissue as shown by silver proteinate staining (1981)
    Springer
    Histochemistry and cell biology 73 (1981), S. 429-438 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of chloroquine on the intralysosomal degradation of cell-coat glycoproteins in cultured intestinal absorptive cells was investigated by silver proteinate staining. The results of this staining method, which is specific for carbohydrate containing macromolecules such as glycoproteins and mucopolysaccharides, showed that in the presence of the drug considerable amounts of silver proteinate-positive material accumulated in one type of lysosome-like body: the dense bodies. The staining pattern of other cell organelles was not affected by chloroquine. The presence of the drug in the culture medium also resulted in the occurrence of numerous small vesicular structures in the matrix of the dense bodies. These showed a similar size and structure to those present in the other type of lysosome-like body: the multivesicular bodies. This observation, together with earlier autoradiographical data, suggests that cell-coat material is transferred from multivesicular to dense bodies by fusion between these organelles. This study thus provides further evidence for a regulatory mechanism of cell-coat glycoprotein transport by the lysosome-like bodies in human intestinal absorptive cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495657
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  • 2
    Electronic Resource
    Electronic Resource
    Heterogeneity in 5′-nucleotidase activity of mouse peritoneal macrophages (1983)
    Springer
    Histochemistry and cell biology 79 (1983), S. 295-309 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary After stimulation of the mouse peritoneal cavity with newborn calf serum (NBCS), four types of monocyte and macrophage were distinguished on the basis of peroxidase (PO) patterns. Cytochemically, these cells showed strong heterogeneity in 5′-nucleotidase (5′N) activity. Monocytes and monocyte-derived macrophages with PO activity in granules lacked 5′N activity. Resident macrophages (with PO activity in RER and nuclear envelope) generally had significant 5′N activity on the plasma membrane, the pattern showing close correlation with the biochemical findings. The group of PO-negative macrophages comprised both 5′N-negative and 5′N-positive cells. These findings suggest two possibilities, i.e., that monocytes (5′N-)transform via PO-negative cells (5′N-/+) into resident macrophages (5′N+), or that the monocytes and monocyte-derived macrophages and the resident macrophages represent separate lineages. The fourth type of macrophage, the exudate-resident cell (with PO activity both in granules and in the RER and nuclear envelope), occurred only in low numbers and very late after NBCS stimulation, and is therefore considered not to be a transitional cell between monocytes and resident macrophages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00491767
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  • 3
    Electronic Resource
    Electronic Resource
    A cytochemical method for the demonstration of 5′-nucleotidase in mouse peritoneal macrophages, with cerium ions used as trapping agent (1982)
    Springer
    Histochemistry and cell biology 75 (1982), S. 437-443 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A method was developed for the demonstration of 5′-nucleotidase in murine peritoneal resident macrophages. The cells are incubated cytochemically without agitation and cerium chloride is used as a trapping agent. Under these conditions, the great majority of the macrophages in the unstimulated peritoneal cavity show enzyme activity in the plasma membrane. In the presence of AMP-S (an AMP analogue inhibiting 5′-nucleotidase, as shown biochemically) there was a decrease in both the number of positive macrophages and the amount of reaction product on the plasma membranes. This indicates that the enzyme activity detected by our cytochemical procedure is attributable to 5′-nucleotidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00640596
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  • 4
    Electronic Resource
    Electronic Resource
    Binding of cationized ferritin to the cell-coat glycoproteins of human and rat small-intestinal absorptive cells (1980)
    Springer
    Histochemistry and cell biology 69 (1980), S. 131-135 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The binding of cationized ferritin (CF) to the cell-coat (glycocalyx) glycoproteins of human and rat intestinal absorptive cells was investigated in relation to the amount of sialic acid in these macromolecules. The cell coat of human absorptive cells exhibited poor binding of CF and contained a small amount of sialic acid. The cell coat of rat absorptive cells had about ten times more sialic acid than that of human cells and showed a strong affinity for the marker. The removal of sialic acid from the cell-coat glycoproteins of rat intestinal cells by neuraminidase treatment abolished CF binding. These results suggest that sialic acid is necessary for CF binding and that human and rat intestinal absorptive cells show a species-specific difference in the sugar composition of the cell coat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00533129
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  • 5
    Electronic Resource
    Electronic Resource
    The hyperfine structure and nuclear moments of some neutron-deficient as isotopes (1980)
    Springer
    The European physical journal 294 (1980), S. 1-5 
    Details
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The hyperfine structure of the4 S 3/2-electronic ground state of the neutron-deficient isotopes69–72As has been measured in an atomic beam magnetic resonance experiment. Nuclear moments have been derived using experimental values of hyperfine interaction constants and nuclear moments of the stable isotope75As. The moments are compared with results from nuclear model calculations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01473115
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  • 6
    Electronic Resource
    Electronic Resource
    Gas-Liquid mass transfer in fixed-bed reactors with cocurrent downflow operating in the pulsing flow regime (1984)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 30 (1984), S. 393-401 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Liquid-side mass transfer coefficients were measured for cocurrent two-phase downflow in 5 and 10 cm diameter columns pakced with 2.5 and 4 mm Raschig rings. Experiments were specifically carried out in the pulsing flow regime. The mass transfer coefficients were determined via absorption of CO2 into buffer solutions with the advantage of a high absorbing capacity. Thus columns of 1 m length could be used. Relations are proposed based on the hydrodynamic phenomena observed in pulsing flow. From these relations a correlation for kL is found in terms of flow rates and packing characteristics that satisfies the experimental data.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690300307
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  • 7
    Electronic Resource
    Electronic Resource
    Endocytosis in absorptive cells of cultured human small-intestinal tissue: Horseradish peroxidase, lactoperoxidase, and ferritin as markers (1981)
    Springer
    Cell & tissue research 216 (1981), S. 1-13 
    Details
    ISSN: 1432-0878
    Keywords: Endocytosis ; Intestinal absorptive cell (human) ; Horseradish peroxidase ; Lactoperoxidase ; Ferritin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The occurrence of endocytotic mechanisms in human small intestinal absorptive cells was investigated by culturing biopsy specimens in the presence of horseradish peroxidase (HRP), lactoperoxidase (LPO), and ferritin. The results indicate that both HRP and LPO entered the cells by apical endocytosis, after which they were transported via apical vesicles and tubules to the lysosome-like bodies. Ferritin, which showed a distinct affinity for the cell-coat glycoproteins, was not interiorized by the absorptive cells. These findings suggest that although human absorptive cells have an endocytotic mechanism, possibly fluid-phase endocytosis, cell-coat glycoproteins are not taken up by the cells, as indicated by the absence of ferritin in the apical vesicles and tubules, as well as the lysosome-like bodies. These findings provide indirect support for our hypothesis that the lysosome-like bodies have a function in the regulation of cell-coat glycoprotein transport via a crinophagic mechanism (fusion of apical vesicles and tubules with lysosome-like bodies) rather than via an exocytotic-endocytotic mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234540
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  • 8
    Electronic Resource
    Electronic Resource
    Endocytosis in absorptive cells of cultured human small-intestinal tissue: Effect of cytochalasin B and D (1982)
    Springer
    Cell & tissue research 222 (1982), S. 113-126 
    Details
    ISSN: 1432-0878
    Keywords: Cytochalasin B and D ; Endocytosis ; Intestine (human) ; Glycoprotein transport ; Lysosomal degradation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of cytochalasin B (CB) and cytochalasin D (CD) on the endocytotic uptake of horseradish peroxidase (HRP) by intestinal absorptive cells was investigated by morphometric methods. The results showed that CD inhibited endocytosis considerably, and without any detrimental side-effects. CB had hardly any effect on the endocytosis of HRP, but caused a significant decrease in the number of apical vesicles and tubules involved in the transport of cell-coat glycoproteins from the Golgi apparatus to the brush border. Electron-microscopic autoradiographic analysis of the effect of CD showed that although endocytosis is inhibited significantly by the drug, the amount of radiolabelled cell-coat material entering the lysosome-like bodies was unaltered compared with control cultures. These observations support our hypothesis that the cell-coat glycoproteins of the absorptive cells enter the lysosome-like bodies by a crinophagic rather than by an exocytotic-endocytotic mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218292
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  • 9
    Electronic Resource
    Electronic Resource
    The effect of chloroquine on lysosomal function and cell-coat glycoprotein transport in the absorptive cells of cultured human small-intestinal tissue (1981)
    Springer
    Cell & tissue research 218 (1981), S. 227-251 
    Details
    ISSN: 1432-0878
    Keywords: Chloroquine ; Human enterocyte ; Glycoprotein transport ; Lysosomal degradation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of chloroquine, an inhibitor of intralysosomal catabolism, on the synthesis, transport, and degradation of cell-coat glycoproteins in absorptive cells of cultured human small-intestinal tissue was investigated by morphometrical, autoradiographical, and biochemical methods. Neither synthesis nor transport of cell-coat material was affected by the drug, but culturing of the absorptive cells in the presence of chloroquine led to a dose- and time-dependent enlargement of the dense bodies; other cell structures showed no alterations. 3H-fucose-labelled material accumulated in the dense bodies of the absorptive cells of these cultures. Since no increase of β-glucuronidase and acid phosphatase activity (both lysosomal enzymes of glycoprotein nature) was found, this accumulation of radiolabelled material can be explained as a chloroquine-mediated inhibition of the degradation of cell-coat glycoproteins. These macromolecules probably enter the lysosome-like bodies by a crinophagic mechanism, i.e., fusion of these organelles with the apical vesicles and tubules involved in intracellular transport. These findings suggest that the lysosome-like bodies have a function in the regulation of cell-coat glycoprotein transport in human intestinal absorptive cells, i.e., the degradation of excess cell-coat material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210340
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  • 10
    Electronic Resource
    Electronic Resource
    The effect of colchicine on the intracellular transport of 3H-fucose-labelled glycoproteins in the absorptive cells of cultured human small-intestinal tissue (1981)
    Springer
    Cell & tissue research 215 (1981), S. 1-12 
    Details
    ISSN: 1432-0878
    Keywords: Colchicine ; Human enterocyte ; Glycoprotein transport ; Autoradiography ; Biochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of colchicine on the intracellular transport of 3H-fucose-labelled glycoproteins in the absorptive cells of cultured biopsy specimens of the human intestine was investigated by light- and electron-microscopical autoradiography and by biochemical methods. The results showed a decrease in the radioactivity of the cell coat on the microvilli and an increase in the Golgi apparatus and in the apical vesicles and tubules. This divergence is attributed to a colchicine-induced impairment of the normal transport of cell-coat glycoproteins from the Golgi apparatus, via the apical vesicles and tubules, to the apex of the cell. The radioactivity of the lysosome-like bodies in the absorptive cells cultured with colchicine also increased. This finding supports a crinophagic function of these organelles in the degradation of excess cell-coat material. The investigations were supported in part by the Foundation for Medical Research (FUNGO), which is subsidized by the Netherlands Organisation for the Advancement of Pure Research
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236244
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