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Raman spectroscopic study of Streptomyces subtilisin inhibitor (1982)

Harada, I. ; Takeuchi, H. ; Tasumi, M. ; [et al.]

Chichester [u.a.] : Wiley-Blackwell

Journal of Raman Spectroscopy 13 (1982), S. 276-279

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Details

ISSN: 0377-0486

Keywords: Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Physics

Notes: Raman spectra of Streptomyces subtilisin inhibitor (SSI) in H2O and D2O solutions were observed for the first time. Information obtained regarding the conformations of polypeptide backbone, disulphide bridge and methionyl side chain was largely consistent with the results derived from other methods. As for the microenvironments of the three tyrosyl residues, the Raman spectrum suggested that none of these residues were involved in a specific interresidue interaction as proposed by nuclear magnetic resonance study for Tyr-93. The Raman spectrum was shown to be useful for studying the binding of the sulphate ion to SSI.

Additional Material: 1 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/jrs.1250130313

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