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  • 1
    ISSN: 1432-2242
    Keywords: Electrophoresis ; Endosperm ; Proteins ; Emmer ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Triticum turgidum L. var. dicoccoides (wild emmer) is an important genetic resource for increasing the protein content of common wheat (Triticum aestivum L.). Many studies have shown that the presence or absence of bands in sodium dodecyl sulfate polyacrylamide (SDS-PAGE) electrophoregrams of wheat storage proteins to be of a purely genetic character. A total protein extraction and SDS-PAGE technique was used to estimate the storage protein genetic variability among 841 accessions of wild emmer collected from various ecological regions in the Middle East. In addition, a computer data bank was developed, recording the onedimension electrophoregram bands for each accession by molecular weight (MW) and relative Coomassie Blue staining intensity as determined from densitometer scans. Analyses of this information are being used to identify specific accessions for further study by two dimension electrofocusing-electrophoresis and breeding and genetic analyses. The computer-assisted analyses indicated that the greatest genetic variability occurs for proteins in the high MW region (above 70,000 MW) followed by those in the medium range (70,000 to 33,300 MW). Comparatively little variability was revealed for protein subunits of below 33,300 MW.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 33 (1984), S. 273-280 
    ISSN: 1573-5060
    Keywords: Erysiphe graminis tritici ; powdery mildew ; Triticum dicoccoides ; wild emmer ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wild emmer from 73 collection sites, including 107 accessions from Israel, two from Lebanon and one from Turkey, were evaluated for resistance to powdery mildew in field nurseries in Israel and the Netherlands. The wild emmer entries displayed a diversity of responses to powdery mildew infection, ranging from high resistance to complete susceptibility. Most entries were resistant in at least one of the nurseries; several entries proved to be resistant in all the tests. Comparing the reactions of 47 wild emmer accessions tested in six nurseries, 11 markedly different patterns were discerned, indicating the probable presence of several different resistance genes. Genes for resistance to powdery mildew appear to be very common in wild emmer indigenous to Israel. Resistance was found in accessions from most collection sites, in all the geographic regions represented in the collection. The common occurrence of resistance and the apparent diversity of genotypes makes wild emmer a rich gene-pool for resistance to powdery mildew. Since genes for resistance to wheat pathogens can be quite readily transferred to cultivated wheat, wild emmer may be utilized as a valuable source of powdery mildew resistance in wheat breeding.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-5060
    Keywords: Triticum dicoccoides ; wild emmer wheat ; Puccinia striiformis ; yellow rust ; stripe rust of wheat ; minor genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Seven single-plant selections of wild emmer, with temperature-sensitive minor-effect genes for stripe rust resistance, were intercrossed in eight combinations. The resulting progenies were studied for a possible additive gene action. The transgressive segregation towards resistance in F2 observed in all the combinations indicates that additive gene action for resistance indeed occurs in wild emmer. The common occurrence of this phenomenon in random combinations suggests further that several minor-effect genes are involved. Following selection of the most resistant plants in F2, a marked shift towards resistance was noted in F3, which demonstrates a positive response to selection. In some instances, additive resistance selected for (in F2) at the high temperature-profile was expressed (in F3) also at the low temperature-profile. This kind of resistance, when utilized in breeding programmes, promises therefore to be effective over a range of temperatures.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5060
    Keywords: Triticum dicoccoides ; wild emmer wheat ; Puccinia striiformis ; yellow rust of wheat ; stripe rust of wheat ; temperature-sensitive genes ; minor genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Twenty-six selections of wild emmer collected at different sites in Israel and three entries obtained from Turkey were studied for the possible presence of temperature-sensitive genes controlling reaction to Puccinia striiformis. In tests carried out in Israel with a local P. striiformis isolate at two temperature regimes, 16 selections showed a change in infection rating toward resistance at the higher temperature regime. In comparable tests performed in the United States with a Montana P. striiformis isolate of different virulence pattern-including 14 of the same selection—ten entries displayed a temperature-sensitive reaction. Although slightly different temperature regimes were used in Israel and the United States, the results obtained were in general agreement. The shift toward resistance observed in the seedling tests at the higher temperature-profile was also evident in the field in the mature plant stage with increasing spring temperatures.
    Type of Medium: Electronic Resource
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