ISSN:
1432-2013
Schlagwort(e):
[K+]o−[Ca2+]o
;
Electrical stimulation
;
Pentetrazol
;
Epilepsy
;
Sensorimotor cortex
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Abstract Changes in [Ca2+]o and [K+]o were measured in the sensorimotor cortex of cats during repetitive electrical stimulation and during pentetrazol induced epileptiform activity. Repetitive stimulation of the thalamic ventrobasal complex (VB) or of the cortical surface (CS) caused decreases in [Ca2+]o by up to 0.45 mM and increases in [K+]o by up to 7 mM. Maximum reductions of [Ca2+]oΔ[Ca2+]o were found in depths of 100 to 300 μm below cortical surface, while rises in [K+]o were largest in depths of 600 to 1000 μm dependent on stimulation site. At depths below 700–900 μm increases in [K+]o were often accompanied by rises in [Ca2+]o of about 0.2 mM. Pentetrazol (PTZ) when injected at doses of 25 to 40 mg/kg body weight induced spontaneous seizure activity, which was in about 40% preceeded by a slight fall of baseline [Ca+]o. Repetitive stimulation and spontaneous seizures resulted in Δ[Ca2+]o of up to 0.6 mM, whereas rises in [K+]o remained limited to a ‘ceiling level’ of about 10 mM. After PTZ application, peak Δ[Ca2+]o were found at the same recording sites, but, in contrast to normal cortex, decreases in [Ca2+]o were observed in all cortical layers. The enhanced Ca2+-signals after PTZ application and the observed reductions of [Ca2+]o before seizure onset suggest that PTZ utilizes Ca2+-dependent mechanisms to initiate seizure activity.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00657240
Permalink
