ISSN:
1573-5028
Keywords:
barley
;
α-amylase mRNA
;
gibberellic acid
;
abscisic acid
;
aminoethyl cysteine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The changes in the levels of α-amylase mRNA is barley aleurone layers in response to addition of plant growth regulators have been studied using a cloned α-amylase cDNA as the hybridization probe. An increase in gibberellic acid (GA) concentration in the incubation medium from 10−9 M to 10−6 M results in a progressive increase in α-amylase mRNA concentration in the aleurone cells. Detectable levels of α-amylase mRNA appear in the aleurone cells as early as 1 h after addition of GA. The concentration of this mRNA increases for several hours and then declines rapidly. Abscisic acid (ABA) and the amino acid analog S-2-aminoethyl-L-cysteine (AEC) suppress the GA-mediated induction of α-amylase. These compounds appear to affect the level of α-amylase mRNA in aleurone cells as measured byin vitro translation assays and by analysis of RNA blots with α-amylase cDNA probes. It is concluded that the regulation of α-amylase gene expression by ABA is at the level of transcription. Further, a protein factor appears to be required in addition to GA for transcription of α-amylase genes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01578643
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