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  • 1
    Electronic Resource
    Electronic Resource
    Immunohistochemical demonstration of the localization of corticotropin releasing factor-containing neurons in the hypothalamus of mammals including primates (1982)
    Springer
    Anatomy and embryology 165 (1982), S. 303-313 
    Details
    ISSN: 1432-0568
    Keywords: CRF neurons ; Hypothalamus ; Immunohistochemistry ; PAP ; Mammals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The presence of the CRF-containing neurons in the hypothalamus was investigated in four different species (cats, dogs, pigs, and monkeys) by the peroxidase-antiperoxidase technique using specific anti-serum to CRF. In all animals examined, CRF-containing perikarya were found mainly in the paraventricular and supraoptic nuclei, and a small number of the immunoreactive cells were observed in the accessory supraoptic nucleus and the lateral hypothalamic area. The size of the CRF-containing perikarya ranged from 20–35 μm in diameter. These findings suggest that the magnocellular paraventricular and supraoptic nuclei are the center not only of the classical neurosecretory system for the production of the posterior lobe hormones, but also that of the CRF neuronal system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305569
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  • 2
    Electronic Resource
    Electronic Resource
    Immunohistochemical demonstration of serotonin nerve fibers in the neocortex of the monkey (Macaca fuscata) (1983)
    Springer
    Anatomy and embryology 166 (1983), S. 155-168 
    Details
    ISSN: 1432-0568
    Keywords: Serotonin ; Neocortex ; Visual cortex ; Monkey ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using a peroxidase-antiperoxidase immunohistochemical method, the distribution of serotonin nerve fibers was studied in the neocortex of the monkey (Macaca fuscata). All layers of the neocortex showed evidence of serotonin fibers, both fine and thick. Unlike the distribution in rodents, different patterns of distribution were noted in each neocortex of the primate. Among the cortical areas — area 4, 3-1-2, 17, 18, 41 and 42 — the primary visual cortex (area 17) contained the highest density of immunoreactive fibers, while the primary motor cortex (area 4) possessed the lowest concentration. The most outstanding finding was a dense and laminar distribution of serotonin fibers in area 17, particularly within the upper portion of layer IVc. In area 3-1-2, 18, 41 and 42, a fairly uniform density of immunoreactive fibers was observed across the six cortical layers, apart from a relatively dense plexus of fine serotonin fibers in layer IV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305080
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  • 3
    Electronic Resource
    Electronic Resource
    Serotonin distribution in the circumventricular organs of the rat (1983)
    Springer
    Anatomy and embryology 167 (1983), S. 311-319 
    Details
    ISSN: 1432-0568
    Keywords: Serotonin ; Circumventricular organs ; Rat ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Serotonin-immunoreactive structures in the circumventricular organs (organum vasculosum laminae terminalis, subfornical organ, subcommissural organ and area postrema) of the rat were demonstrated using a modified peroxidase-antiperoxidase immunohistochemical method. Various densities of serotonin fibers were demonstrated in all four circumventricular organs; however, serotonin-positive cells were evident in the area postrema only after nialamide treatment. Serotonergic supraepedymal fibers were observed on the surface of the organum vasculosum laminae terminalis and that of the subfornical organ, but not on the subcommissural organ and area postrema. The serotonergic plexus of the basal portion of the subcommisural organ was considered to be continuous with the supraependymal plexus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315669
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  • 4
    Electronic Resource
    Electronic Resource
    Serotonergic innervation on the motoneurons in the mammalian brainstem (1983)
    Springer
    Anatomy and embryology 167 (1983), S. 321-333 
    Details
    ISSN: 1432-0568
    Keywords: Serotonin ; Immunohistochemistry ; Brainstem ; Motoneuron ; Monkey
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A comparative study of serotonergic innervation on motoneurons in the brainstem of various mammals (mouse, rat, guinea pig, dog, cat and monkey) was carried out using a sensitive immunohistochemical method. Except for the extraocular muscle nuclei, the motor nuclei of the cranial nerves received rich inputs from serotonin neurons, in all species examined — rodent, carnivore and primate. The motoneurons of the monkey were innervated by varicose serotonin fibers, in a manner different from that of other species, i.e. their cell bodies and proximal dendrites were tightly encircled by a large number of serotonin-containing varicose fibers. At the ultrastructural level, a predominant population of axosomatic contacts was confirmed in the cranial motor nuclei of the monkey, particularly in the nucleus ambiguus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315670
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  • 5
    Electronic Resource
    Electronic Resource
    Sexual differences in the topographical distribution of serotonergic fibers in the anterior column of rat lumbar spinal cord (1984)
    Springer
    Anatomy and embryology 170 (1984), S. 117-121 
    Details
    ISSN: 1432-0568
    Keywords: Sexual dimorphism ; Serotonin fibers ; Lumbar cord ; Cremaster ; Immunohistochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Sexual dimorphism in the topographical organization of immunoreactive serotonergic fibers has been shown for the first time in the anterior column of the rat lumbar cord. A characteristic preferential arrangement of serotonergic fibers on the small cell column composed of anterior horn motoneurons, which have been proved to send their axons to the cremaster muscle, was demonstrated at the lumbar segments (L1–L2) of male rats, using the immunoperoxidase technique with antiserum against serotonin. A similar finding was also observed in female rats, but was less prominent than in males. Comparative analysis of the cell numbers and the size of neuronal somata of the aforementioned nucleus, done using retrograde transport of fluorescent dye (DAPI) via axonal fibers coursing down the genitofemoral nerve to the cremaster muscle, gave significantly larger values in males. The sexual difference in the serotonergic innervation pattern was, in consequence, surmised to be caused by the cytoarchitectonic contrast ascertained in the lumbar anterior column. Furthermore, there may be a striated muscle endowed with some active functions homologous to those of the male cremaster muscle in the female rat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318995
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  • 6
    Electronic Resource
    Electronic Resource
    Improved Kakioka automatic standard magnetometer (KASMMER) (1984)
    Springer
    Surveys in geophysics 6 (1984), S. 357-365 
    Details
    ISSN: 1573-0956
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The KASMMER (Kakioka Automatic Standard Magnetometer) has been constructed in 1972 for the standard magnetic observation at Kakioka (Yanagiharaet al., 1973). Then KASMMER system has been improved successively for the high level standard magnetic observation. The purpose of the present paper is to introduce an outline of the improved KASMMER system. The KASMMER system consists of four parts which are four optical pumping magnetometers, a fluxgate magnetometer (Supporting system to the optical pumping magnetometers), a calibration system and a data acquisition system (computer system). The calibration system consists of a proton magnetometer and a magnetic theodolite (Di-72). Details for each part are described and the ability of the improved system is shown to be higher than that of classical magnetic observatory systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01465551
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  • 7
    Electronic Resource
    Electronic Resource
    Fluorescence histochemical study on the noradrenergic control to the anterior column of the spinal lumbosacral segments of the rat and dog, with special reference to motoneurons innervating the perineal striated muscles (Onuf's nucleus) (1984)
    Springer
    Histochemistry and cell biology 81 (1984), S. 237-241 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The organization of noradrenergic fibers in the lumbosacral anterior column of rats and dogs was examined in detail using a modification of a highly sensitive glyoxylic acid fluorescence histochemical method. In both rat and dog, there were greater concentrations of fluorescing noradrenergic fibers around the motoneurons innervating the perineal striated muscles (Onuf's nucleus) than around other motoneuronal groups. The preferential accumulation of noradrenergic fibers in Onuf's nucleus may indicate that the noradrenergic neuron system in the spinal cord of rodents and carnivores is closely related to the functional peculiarities of the perineal striated muscles, including the external anal and urethral sphincter muscles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495633
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  • 8
    Electronic Resource
    Electronic Resource
    Immunohistochemical demonstration of serotonin-containing CSF-contacting neurons in the submammalian paraventricular organ (1983)
    Springer
    Histochemistry and cell biology 77 (1983), S. 423-430 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution and morphological aspects of the serotonin-containing neurons in the paraventricular organ of the carp, frog, turtle and chicken were studied by means of an immunoperoxidase technique using serotonin antiserum. In all species the serotonin-containing neurons were seen to have the appearance of the CSF-contacting neurons and to be distributed in the pars ependymalis and the pars hypendymalis of the organ. Particularly, in the frog, the serotonin-containing CSF-contacting neurons, mostly bipolar in shape, were also observed in the pars distalis. Their proximal processes protruded into the ventricular lumen through the ependymal layer with a globular- and triangular-shape. The distal processes projected ependymofugally to the pars distalis and formed a fine plexus in the neuropil of this part. The density of the serotonin fibers in the pars distalis was greater in the carp than in the other species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495798
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  • 9
    Electronic Resource
    Electronic Resource
    Identification of EC cells in the human intestine: A comparative study between immunohistochemical and silver impregnation techniques (1983)
    Springer
    Histochemistry and cell biology 79 (1983), S. 9-16 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Selective immunohistochemical identification of enterochromaffin (EC) cells in the human duodenum and colon has been carried out, using a highly specific rabbit antiserotonin serum and the unlabeled peroxidase-antiperoxidase complex (PAP) technique. The sensitivity of the reaction, and morphology of the cells, were compared with those of the argentaffin or argyrophil reactions on the same sections at the light microscopic level. All argentaffin cells were argyrophil and PAP positive, but a small number of PAP-positive cells were neither argentaffin nor argyrophil. In addition, a few EC cells stained by the PAP method were shown to be argyrophil but not argentaffin. The PAP method permitted the clear morphological identification of the entire cell, particularly its cytoplasmic processes, whereas both the argentaffin and argyrophil reactions were basically seen in the basal regions of the cells and often failed to demonstrate the cytoplasmic processes. These findings indicate that the immunohistochemical method is more sensitive and advantageous than silver impregnations for the selective demonstration of EC cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00494337
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  • 10
    Electronic Resource
    Electronic Resource
    Serotonin immunohistochemistry reveals immature EC cells (1984)
    Springer
    Histochemistry and cell biology 80 (1984), S. 517-518 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Radioautographic studies of rat duodenal mucosa were carried out after single injections of 3H-thymidine, and the labelling indices of enterochromaffin (EC) cells identified by serotonin immunohistochemistry and by the diazo reaction were compared. The labelling index of EC cells by serotonin immunohistochemistry was 2.3% (mean percentage of 2 rats), while no labelled cells were found in diazo-positive EC cells. This finding indicates that serotonin immunohistochemistry reveals a less mature population of EC cells than conventional techniques such as the diazo reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495444
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