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  • 1
    Electronic Resource
    Electronic Resource
    Physical properties and function of phallolysin (1983)
    s.l. : American Chemical Society
    Biochemistry 22 (1983), S. 4574-4580 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00288a035
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  • 2
    Electronic Resource
    Electronic Resource
    Cytochemistry and biochemistry of acid phosphatases (1980)
    Springer
    Histochemistry and cell biology 67 (1980), S. 99-111 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Acid phosphatases of the rat ventral prostate were studied cytochemically using different substrates. The results were compared to findings on isoelectric focussing gels stained for acid phosphatase activity. This is a highly specific and reproducible method which allows the distinction between secretory androgen-dependent and lysosomal acid phosphatases. Activity of lysosomal acid phosphatase was increased after castration, while the activity of the secretory enzyme gradually decreased after androgen deprivation. None of the substrates tested was selectively hydrolyzed by either secretory or lysosomal acid phosphatase. Phenylphosphate, creatine phosphate and choline phosphate were found to be inappropriate substrates for histochemical purposes, however, reproducible results were obtained with α-naphthylphosphate, β-glycerophosphate and p-nitrophenylphosphate. The method of isoelectric focussing (pH range 4.0–8.0) of enzymes with subsequent histochemical staining demonstrated lysosomal enzymes at pH 7.9 and 8.2 respectively. Small amounts of identical enzymes were found in liver, kidney, blood or epididymis. Secretory acid phosphatases were focussed at pH 5.5, 5.6, 5.65 and 7.15. Similar enzymes have been identified in epididymis, kidney, liver and pancreas. These results indicate that 1) at present no “specific” substrate for prostatic secretory or lysosomal acid phosphatases is available and 2) that no prostate-specific “prostatic acid phosphatase (PAP)” exists in the rat ventral prostate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00490092
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  • 3
    Electronic Resource
    Electronic Resource
    Intracellular localization of prostatic binding protein (PBP) in rat prostate by light and electron microscopic immunocytochemistry (1982)
    Springer
    Histochemistry and cell biology 76 (1982), S. 497-516 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Extra- and intracellular distribution of Prostatic Binding Protein (PBP) was studied in the different genital organs of the male rat by immunocytochemistry at the light and electron microscopic levels. PBP was extracted from cytosols of rat ventral prostate and used for immunization of rabbits. The specificity of the antiserum raised was tested by “western blotting” and immunoelectrophoresis. From the different fixatives tested for optimal structural and antigenic preservation of the ventral prostate a mixture containing 2.5% paraformaldehyde, 0.5% glutaraldehyde and 0.5% CaCl2 in cacodylate buffer, 0.05 M, pH 7.3 was selected. Using the immunofluorescence technique and the unlabeled antibody enzyme method PBP-immunoreactivity was detected at the light microscopic level in the luminal secretions of the ventral prostate. No reaction was observed with the seminal vesicle, the coagulating gland, the dorsal and lateral prostates, the epididymis and the testis. Intracellular secretory granules reacting with PBP antiserum were exclusively found in the secretory cells of the ventral prostate. Insufficiently fixed cells showed a diffuse generalized reaction of the cytoplasm indicating a leakage of the antigen from the secretory granules. Such artifacts were common in tissue sections processed with the preembedding-staining procedure. At the ultrastructural level therefore mostly the postembedding staining method was performed using both the unlabeled antibody enzyme method and the ferritin-labeled immunoglobulin technique in osmicated, Epon-embedded tissue. Labeling with either method was intense in the secretory granules and the condensing vacuoles, while the labeling density of the rough endoplasmic reticulum and the Golgi cisternae was in the background range. Castration experiments showed that secretory material displaying PBP immunoreactivity was retained within the acinar lumen of the gland for several days after castration, but was absent from most secretory cells already by four days after castration. Immunocytochemistry of PBP therefore is a very sensitive method for analysing the secretory activity and its androgen dependence of the prostate of the rat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00489905
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  • 4
    Electronic Resource
    Electronic Resource
    Arterial $$Pa_{CO_2 }$$ during chronic hyperthermia in sheep (1980)
    Springer
    Pflügers Archiv 384 (1980), S. 143-147 
    Details
    ISSN: 1432-2013
    Keywords: Acid-base balance ; pHa ; Bicarbonate ; Thermal polypnea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study was undertaken to determine if the decrease in $$Pa_{CO_2 }$$ and the concomitant increase in pHa seen during acute (less than 24 h) hyperthermia in all mammals was modified when the hyperthermia was maintained for periods longer than 1 day. Catheters were placed in the descending aortas of anesthetized ewes (pentobarbital, 30 mg/kg, i.v.). After recovery from the surgery, arterial blood samples were drawn daily during a 7 day control period and an 8 day period of continuous hyperthermia. In all animals $$Pa_{CO_2 }$$ decreased and remained low during the entire hyperthermic period. $$Pa_{CO_2 }$$ (torr) andT r (°C) were inversely correlated by the equation: $$Pa_{CO_2 }$$ = −6.08T r+267.8 (r=0.84). There was an initial alkalosis with hyperthermia, however pH tended to decrease after the fifth day of hyperthermia. Calculated bicarbonate decreased during hyperthermia. The evidence suggested that when body temperature was increased in sheep, $$Pa_{CO_2 }$$ was maintained at a lower value. The low $$Pa_{CO_2 }$$ value was maintained independent of changes in pHa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00584430
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  • 5
    Electronic Resource
    Electronic Resource
    p-Chlorophenylalanine-induced proliferation of the seminal vesicle epithelium (1981)
    Springer
    Cell & tissue research 219 (1981), S. 159-172 
    Details
    ISSN: 1432-0878
    Keywords: Seminal vesicles ; Proliferation ; Autoradiography ; Biochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Intraperitoneal injection of p-chlorophenylalanine (pCPA) methylester (100 mg/kg body weight) results in an activation of the lysosomal system of the secretory cells in the rat seminal vesicle and an elevation of the activities of lysosomal enzymes within 15 min following the injection. Large autophagic vacuoles are formed, sequestering rough endoplasmic reticulum and part of the Golgi apparatus within 2 h. Shortly after the activation of the lysosomal system an elevation of both DNA and protein synthesis is measured biochemically. 6 h subsequent to the injection a wave of mitoses of the secretory cells begins, reaching a maximum 6 h later and then declining within 3 h. About 12 h following the injection a second rise in lysosomal activity begins, declining within 24 h. The entire sequence of lysosomal and proliferative activities is inhibited in antiandrogen-pretreated rats. Deduced from these findings the following hypothesis of growth regulation of the accessory sex glands is advanced: enhanced loss of intracellular material during autophagocytosis diminishes the intracellular concentration of a substance curtailing cell division below its effective threshold resulting in division of the secretory cells. The prerequisites of this mechanism are (i) a sufficient distributive capacity of the stroma for hormones (androgens) and metabolic precursors, and (ii) sufficient capacity of the basal cells for transporting the precursors to the secretory cells. Sloughing of the secretory cells separates them from these auxiliary structures (stroma and basal cells) and enables the basal cells to divide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210025
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