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  • 1980-1984  (7)
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 39 (1984), S. 0 
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Small plots of permanent pasture containing 50%Agrostis capillaris-Festuca rubra were defoliated frequently or infrequently during the growing seasons in 1978–80 and fertilized with 133, 125 and 125 kg N, P2O5 and K2O respectively ha−1 a −1 or not fertilized during 1978–81. Yields of herbage were assessed during 1979–80 and botanical composition assessed on three occasions in 1978, once in April 1979 and once in 1982.Fertilizer application increased annual dry matter (DM) harvested from frequently cut plots by 40%, 53% and 65% in 1978, 1979 and 1980 respectively, and on infrequently cut plots it doubled hay DM harvested in 1978 and increased total DM harvested by 126% and 186% in 1979 and 1980 respectively. Infrequent cutting and fertilizer both decreased the proportion of A. capillaris but only fertilizer decreased the proportion of F. rubra. Fertilizer greatly increased the proportion of Holcus lanatus especially where plots had been cut frequently and of Alopecurus pratensis where they had been cut infrequently. It decreased the proportion of Luzula campestris. Infrequent cutting, especially with fertilizer, discouraged Cerastium fontanum ssp. glabrescens and Trifolium repens but encouraged Rumex acetosa.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 39 (1984), S. 0 
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The establishment and growth of white clover (cv. Grasslands Huia) was investigated in two experiments with seeds sown in slots cut in old permanent pasture in early May 1982. In the first experiment, fully protected from pests and diseases, either 50 (low), 100 (medium) or 200 (high) seeds were sown evenly per metre length of slot; 100 seeds were also sown in a clumped pattern unevenly. In the second experiment, the effects of various combinations of molluscicide, insecticide and fungicide were investigated.Nine weeks after sowing there were 27, 37, 34 and 62 seedlings m−1 on the low, medium even, medium uneven and high seed-rate treatments, representing percentage establishments of 54, 37, 34 and 31 respectively. By September, however, there were no significant differences between the dry weights m−l of any of the treatments. In the second experiment, damage by slugs occurred early in treatments without molluscicide and caused a mean reduction of 70% in seedling establishment. As a result significantly smaller dry weights m−1 of clover were recorded in September in these than in other treatments. Failure to protect against insects resulted in smaller seedlings.The results indicated that variations in seed rate within the range investigated were not critical but that failure to protect against pests decreased percentage establishment and subsequent growth.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1434-0879
    Keywords: Neutron activation analysis ; stone formers ; bendrofluazide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Whole body elemental compostion was measured by in vivo neutron activation analysis before and after treatment of urinary stoneformers with bendrofluazide for 6 months. There was no significant change in whole body calcium, sodium, nitrogen or body weight. There were significant reductions in whole body potassium (6%), chlorine (7.3%) and phosphorus (1.5%).
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rat thyroid follicular cells desensitized to the mitogenic action of TSH by prolonged stimulation retain the capacity to respond to the mitogenic stimulus of wounding. The implications for thyroid growth control and neoplasia are discussed.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular histology 14 (1982), S. 609-620 
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This report describes the preparation and use of an artificial tissue containing differing amounts of an antigen covalently bound to gelatine (step wedge). The use of this step wedge enables the variables in the immunoperoxidase technique to be studied separately. The step wedge may be used as a standard in immunocytochemical quantification. By performing all the steps of the immunoperoxidase procedure on the step-wedge section and the tissue section simultaneously, valid comparisons can be made regarding changes in the amount of antigen in tissues. Absolute measurements of amounts of antigen in tissue cannot be made with this technique at present because of the loss of antigenicity involved in the preparation of the step wedge, differences of penetration by reagents between step wedge and tissue, and non-uniform distribution of antigens in this tissue. Use of the step wedge has shown that, under some circumstances, an increase in amount of antigen may be associated with a reduction in the amount of reaction product, demonstrating the impossibility of making any deductions about variations in the amount of antigen without control of the technique employed by a method such as the one presented here.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Studies of X-linked enzymes provide an approach to the study of tumour and normal cellular development. We have assessed the technique for the histochemical demonstration of one such enzyme, ornithine carbamoyltransferase (EC 2.1.3.3). Various stages in the Mizutani technique for ornithine carbamoyltransferase were re-examined, and the resulting improved technique applied to normal mice and to mice of the sparse fur strain (Spf) known to have an abnormal form of ornithine carbamoyltransferase inherited as an X-linked characteristic. Positive enzyme activity was present in all hepatocytes from normal mice, the strongest reaction being present in the periportal area with a gradual reduction of activity towards the centrilobular region. No activity was demonstrable in hepatocytes from hemizygous male Spf mice. In heterozygous female Spf mice, there was a clear-cut separation of ornithine carbamoyltransferase-positive and-negative cells. These were present in very variable proportions in different liver lobes and different animals. Preliminary studies were also carried out using a high pH reaction mixture to detect the abnormal enzyme. These studies demonstrate conclusively the X-linkage of ornithine carbamoyltransferase in mice, showing the mosaic pattern of distribution predicted by the Lyon hypothesis. They show that the Spf strain of mice can be used for studies of both development and tumorigenesis in the liver, and that histochemical study of an animal strain with an X-linked enzyme abnormalit provides a powerful investigative tool.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular histology 15 (1983), S. 543-555 
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A simple and versatile technique for the preparation of ultra-thin sections, which can be stained immunohistochemically directly on electron microscope grids, is presented. An anti-hapten immunoperoxidase procedure has been adapted for use on tissue fixed in a purified monomeric glutaraldehyde-picric acid mixture, and embedded in ‘L R White’, a recently formulated plastic resin. This plastic tolerates the use of partial dehydration of tissue, resulting in higher antigenic yields. In addition, no etching of ultra-thin sections is necessary, and the whole immunostaining procedure can be completed in less than 2 h. A comparison of commonly used fixatives is discussed. High-resolution micrographs showing general staining (uranyl acetate-lead citrate) of rat pancreas, and immunostaining of insulin and TSH in storage granules in perfusion-fixed rat tissue and of lambda-chain immunoreactive cells in immersion-fixed human tonsil are included as examples.
    Type of Medium: Electronic Resource
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