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  • 1
    Electronic Resource
    Electronic Resource
    Stimulation of canine lymphocyte subpopulations separated nonlytically by monoclonal anti-T and polyclonal Anti-B cell antibodies (1982)
    Springer
    Annals of hematology 45 (1982), S. 309-316 
    Details
    ISSN: 1432-0584
    Keywords: Canine lymphocytes ; Blastogenesis ; Panning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Canine blood lymphocytes were nonlytically separated on antibody-coated petri dishes into surface immunoglobulin-positive (SIg+) and -negative (SIg−) populations. SIg− cells were further separated into cells reactive or non-reactive with monoclonal antibody DT-2 recognizing canine T lymphocytes. The purity of the three enriched lymphocyte populations exceeded 90% as assessed by immunofluorescence. Mitogen stimulation showed a vigorous response of SIg+ cells to pokeweed mitogen and concanavalin A but only a weak response to phytohemagglutinin. In mixed lymphocyte cultures, SIg+ cells were poor responders but potent stimulators. DT-2− and DT-2+ cells responded to phytohemagglutinin, concanavalin A and pokeweed mitogen, and both populations were good responders in mixed leukocyte culture. Only DT-2− cells were potent stimulators; DT-2+ cells were not. Hence, canine blood T cells can be divided into two subsets, DT-2+ and DT-2−, both of which are responsive to mitogens and alloantigens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00319524
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Canine bronchoalveolar cells: Antigen-presenting macrophages are Ia-positive, lymphocytes are of non-B lineage (1983)
    Springer
    Annals of hematology 47 (1983), S. 263-270 
    Details
    ISSN: 1432-0584
    Keywords: Canine bronchoalveolar cells ; Ia antigens ; Monoclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Subsegmental bronchoalveolar lavages were performed in 18 healthy beagles. The average yield per lavage was 45×106 cells consisting on the average of 24% lymphocytes, 71% macrophages, and 4% granulocytes. Cells were further examined in cytofluorometric studies using monoclonal (anti-Ia, antilymphocyte, anti-T) and polyspecific (anti-Ig) antibodies. Sixty to 90% of lymphocytes were T cells as determined by the T cell antibody DT-2. No surface immunoglobulin-positive cells (B cells) could be detected. All macrophages expressed Ia antigens (p 29/34) whereas lymphocytes did not. In assays of concanavalin A-induced blastogenesis of thymocytes, alveolar macrophages functioned as accessory cells. The anti-Ia antibody 7.2 interfered with this function, indicating that Ia antigens on canine alveolar macrophages are involved in interaction with T cells resulting in T cell proliferation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00319895
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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