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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Experiments in fluids 15 (1993), S. 10-16 
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract This paper describes and evaluates a thermal transient anemometer, a novel fluid flow (mass flux) measuring device having a thermocouple probe which is utilized to measure the change in temperature over a period of time to provide a measure of fluid flow velocity. The thermocouple probe (sensor) is periodically heated by the application of an electrical pulse and its rate of cooling between pulses is related to the local mean flow velocity. The theory of operation, device description, comparison with other similar fluid flow devices, and laboratory tests including measurements under different turbulence levels and different fluid temperatures are presented and discussed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Bioprocess and biosystems engineering 6 (1990), S. 49-61 
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The effect of NH4Cl addition on intracellular pH (pH i ) was determined by flow cytometric measurements of the fluorescence of a pH-sensitive dye. The effects of NH4Cl on growth were determined for batch growth of cells in flasks in an incubator. The addition of NH4Cl caused a cytoplasmic acidification. A new lower steady-state value of pH i was attained within 20–40 min of NH4Cl addition. A correlation was found between the effects of NH4Cl on growth and on pH i : whereas 3 mM NH4Cl had little effect on growth and on pH i , 10 mM NH4Cl caused a substantial growth inhibition and a pH i decrease of 0.2–0.3 units. The effects of NH4Cl on growth and on pH i were found to be independent of the external pH value (pH e over the range 6.8 to 7.6, except that 10 mM NH4Cl was more toxic at pH e 7.6. The addition of NH4Cl caused an increase in the average cell volume at pH e 7.6, but had no effect on the average cell volume at pH e 's 6.8 and 7.2. For comparison, the effects of pH e alone on growth and on pH i were determined. There was little difference in cell growth at pH e 's 6.8, 7.2 and 7.6. At pH e 6.6, there was a substantial growth inhibition. Some measurements of the effects of pH e on pH i were made, although the steady-state value of pH i as a function of pH e was not determined due to limitations in the pH i -measuring technique. These measurements showed that pH i remained constant from pH e 7.6 to 6.8, but fell by 0.2 units at pH e 6.6, in agreement with the growth results.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The effects of dissolved oxygen concentration on hybridoma cell growth, metabolism, and antibody production were studied. A mouse hybridoma cell line producing an IgG1 directed at a consensus α-interferon was grown in batch cultures in a 5 dm3 stirred bioreactor at dissolved oxygen (DO) concentrations of 5, 30, 90 and 95% or air saturation. High oxygen tension (95% of air saturation) reduced specific growth rate without affecting cell viability. At lower dissolved oxygen levels, specific growth rates were approximately independent of DO, although changes in mitochondrial function and antibody production were observed. Flow cytometry assays of a fluorescent mitochondria-specific marker (Rhodamine 123) show significant single-cell heterogeneities during late exponential growth and greater average fluorescence in cultures grown at 95% DO. The quantity of cell-surface immunoglobulin, measured by an immunofluorescent flow cytometric technique, was the same at high (95%) and low (5%) dissolved oxygen concentrations. Myeloma cells of the type used in constructing the above hybridoma line were much less sensitive to dissolved oxygen level. Specific respiration rate, pyruvate utilization rate, cytochrome oxidase activity, and succinate-cytochrome c oxidoreductase activity were significantly greater (62–116%) for the hybridoma cells than for the myeloma cells in T-flask cultures.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Solid state phenomena Vol. 32-33 (Dec. 1993), p. 141-142 
    ISSN: 1662-9779
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 42 (1977), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Broiler chicken carcasses were inoculated with Salmonella typhimurium (180–360,000 cells per carcass), then, in a simulated commercial chilling process, were prechilled at 18°C in water containing glutaraldehyde. On broilers that had been prechilled for 30 min in 0.5% glutaraldehyde (pH 8.6), then chilled in slush ice for 20 min, salmonellae were eliminated when the inoculum level per carcass was 250 cells, but not when the level was 360,000 cells. Transfer of salmonellae from inoculated to uninoculated carcasses (cross contamination) was prevented by glutaraldehyde at 0.01% or more in the prechill water, when the inoculum level was 200–300 cells per carcass. It was not prevented when the level was 360.000 cells per carcass. A lo-min urechill in 0.5% alutaraldehvde (pH 8.6) extended- carcass shelf-life at 2°C about 6 days beyond that of controls.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 43 (1978), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Broiler carcasses were sampled by excision of the neck skin with the aid of a special clamp, and by swabbing of the breast skin. Samples from both methods were compared for bacteriological evaluation of carcasses by standard plate counts and by two commercial miniature systems (Millipore and Easicult-TTC). In experiment 1, the excised neck skin method showed higher counts than the breast swab method on carcasses stored at 1.5°C for 7–18 days, but not on day of processing; counts did not differ significantly as a function of culturing and counting system (only standard plating and Millipore were compared) on carcasses stored 7–18 days, but were higher by standard plating than by Millipore on day of processing. In experiment 2, counts did not differ significantly as a function of the two sampling methods or three culturing methods, or between counts on 0 and 7 days of storage of carcasses at 1.5°C. The neck skin excise method combined with a miniaturized culturing system was the fastest means to measure the general bacteriological condition of fresh or stored broiler carcasses.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 589 (1990), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Polar research 8 (1990), S. 0 
    ISSN: 1751-8369
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geography , Geosciences
    Notes: New aeromagnetic data, K-Ar age determinations of dredged marine igneous rocks, as well as other geophysical evidence have shed light on the chronology, nature and evolution of the northern Iceland Plateau. Correspondence between seismic refraction profiles taken on the Jan Mayen Ridge and westward through Jan Mayen Island, suppressed aeromagnetic anomalies, earthquake surface wave studies, and ages of dredged igneous rocks suggest these strata may form an extended region of thickened crust, possibly of Caledonian age, extending westward toward the Kolbeinsey Ridge and northwest to the south wall of the Jan Mayen Fracture Zone.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Medicine 29 (1978), S. 417-426 
    ISSN: 0066-4219
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 42 (1993), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The infection of pea (Pisum sativum) by Colletotrichum truncatum was studied by light and electron microscopy. These investigations were facilitated by use of an Argenteum pea mutant, which has a readily detachable epidermis. Infection pegs emerging from appressoria penetrated epidermal cells directly. Large intracellular primary hyphae formed a dense stromatic mycelium confined within a single epidermal cell. Primary mycelia gave rise to thinner secondary hyphae which radiated into surrounding cells and caused extensive wall dissolution. Melanized sclerotia developed in the centre of chlorotic water-soaked lesions. Acervuli were not observed. Epidermal cells survived initial penetration by primary hyphae, as shown by their ability to plasmolyse and accumulate Neutral red, but all infected cells were dead when the secondary hyphae had formed. Six cultivars of pea were susceptible, but seven other legumes were resistant. A single isoform of polygalacturonase with a pI of 8·3 and apparent Mr of 40000 was purified from culture filtrates and the TV-terminal amino acid sequence determined. The relevance of the results to the taxonomy of C. truncatum and the relationships between infection process and host range are discussed.
    Type of Medium: Electronic Resource
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