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  • 1980-1984  (4)
  • 1975-1979  (7)
  • 1
    Electronic Resource
    Electronic Resource
    Haemoglobin crystals in the midgut of the tick Ornithodorus moubata Murray (1977)
    [s.l.] : Nature Publishing Group
    Nature 266 (1977), S. 536-538 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] O. moubata porcinus ticks (males and females) were taken from the laboratory strain, fed on guinea-pigs and dissected at intervals after the blood meal (0, 2, 4, 6, 12, 15, 30, 85 and 200 d), in 0.7% NaCl solution (for X-ray diffraction and spectroscopy) or without a dissecting fluid (for ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/266536a0
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Rasterelektronenmikroskopische Befunde an netzhautinnenflächen (1977)
    Springer
    Graefe's archive for clinical and experimental ophthalmology 204 (1977), S. 31-37 
    Details
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The findings by scanning and transmission electron microscopy of multiple glial excrescences from the retina into the cortical vitreous body are described. These formations are located in lines above superficial retinal vessels. The findings are compared with those from light microscopy of similar formations. The pathogenesis and the possible significance as retinovitreal attachments for rhegmatogenous defects of the retina and for posterior vitreous detachments are discussed.
    Notes: Zusammenfassung Es werden die rasterelektronenmikroskopischen Befunde und die elektronenmikroskopischen Schnittbilder von multiplen, über oberflächlichen Netzhautgefäßen gelegenen Gliaauswüchsen aus der Netzhaut in die Glaskörperrinde beschrieben. Diese Befunde werden mit lichtmikroskopischen Bildern ähnlicher Bildungen verglichen, ihre Pathogenese diskutiert und ihre mögliche Bedeutung als vitreoretinale Adhärenzen für rhegmatogene Netzhautdefektbildungen und hintere Glaskörperabhebung erörtert.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02387414
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  • 3
    Electronic Resource
    Electronic Resource
    Rasterelektronenmikroskopische Untersuchungen an fibrösen und fibro-gliösen epiretinalen Fibroplasien (1978)
    Springer
    Graefe's archive for clinical and experimental ophthalmology 207 (1978), S. 229-242 
    Details
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es werden die rasterelektronenmikroskopischen Befunde von 16 epiretinalen Fibroplasien mit Netzhautfältelung nach verschiedenen primären Funduserkrankungen dargestellt. In diesem ersten Teil besprechen die Autoren: 1. Fibröse, zellfreie epiretinale Membranen mit und ohne strangförmige Glaskörperadhärenzen. Die Fältelung der Retina kommt durch Glaskörpertraktion und Faserbrücken zustande. 2. Fibröse Membranen, welchen einzelne Gliazellen ein- und aufgelagert sind, ohne daß Zellmembranen entstehen. Auch hier wird die Fältelung durch Kollagenfaserbrücken verursacht. 3. Fibröse Membranen, denen gebietsweise Gliamembranen aufgelagert sind. Die Fasermembranen aller drei Typen werden als residuale Glaskörperrinde interpretiert. Weil sie über allen Faltenherden vorhanden sind und wegen ihrem Verhalten zu den Limitansfalten wird angenommen, daß ihre Kontraktion Ursache der Netzhautfältelung ist. Morphologische Anhaltspunkte für Kontraktion von Gliamembranen oder Faserbildung durch die Glia wurden nicht gefunden.
    Notes: Summary The scanning electron microscope was used to examine 16 cases of epiretinal membrane formation and retinal puckers which occurred in a variety of primary retinal disorders. In this first section the authors describe three types of epiretinal membranes: (1) Fibrous acellular membranes with and without vitreous adhesions. Retinal puckering was caused by vitreous traction with collagen strands binding the residual folds. (2) Fibrous membranes containing isolated glial cells. (3) Fibrous membranes partly covered by sheets of glial cells. In all three forms the acellular fibrous component is believed to represent vitreous cortex remnants. The invariable presence of these fibrous membranes at sites of retinal pucker and their relationship to wrinkled internal limiting membrane suggests that contraction of these membranes is responsible for retinal puckering. Morphological evidence of glial membrane contraction and collagen production by glial cells was not found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00431162
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  • 4
    Electronic Resource
    Electronic Resource
    Rasterelektronenmikroskopische Untersuchungen an pigmentierten epiretinalen Fibroplasien (1979)
    Springer
    Graefe's archive for clinical and experimental ophthalmology 210 (1979), S. 109-120 
    Details
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es werden die licht- und rasterelektronenmikroskopischen Befunde von drei pigmentierten epiretinalen Fibroplasien eines amotiooperierten Auges beschrieben. Es handelt sich um fibro-gliöse Schrumpffaltenherde, deren Epizentrum von einer Schicht aus retinalem Pigmentepithel überlagert wird. Die Zellen zeigen die Oberflächenstrukturen typischer oder leicht degenerativ veränderter Epithelien. Ausgedehnte Gebiete dieser Pigmentepithelmembranen sind pigmentfrei. Anhaltspunkte für direkte oder indirekte Traktion des Pigmentepithels auf die Netzhautinnenfläche liegen nicht vor. Die Herkunft der Zellen und ihre Bedeutung für die Faltenbildung werden diskutiert.
    Notes: Summary The light- and scanning electron microscopic appearance of 3 areas of pigmented epiretinal fibroplasia (pucker) are described in an eye removed following detachment surgery. The centre of each area of pucker consists of a fibroglial sheet covered by retinal pigment epithelium. The surface morphology of these cells resembles typical or slightly degenerated epithelium. Extensive areas of these pigment epithelial sheets were pigmentfree. There was no evidence that the pigment epithelial sheets exerted either direct or indirect traction on the inner surface of the retina. The origin of these cells and their role in the formation of retinal wrinkling is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409997
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  • 5
    Electronic Resource
    Electronic Resource
    Crystallized hemoglobin inRhodnius prolixus after a blood meal on guinea-pig (1983)
    Springer
    Cellular and molecular life sciences 39 (1983), S. 1335-1338 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Several blood-sucking arthropods, after a blood meal, are able to store the hemoglobin from their hosts in a crystalline state in their digestive system5,15,20. Guinea-pig hemoglobin crystallizes in the stomach of the reduviid bugRhodnius prolixus in two different crystal types. We show them to be crystallographically identical and to contain the same liganded state of hemoglobin, i.e. they represent different habits of the same crystal modification. The hemoglobin crystallizes in oxy-form and ages in the crystalline state, first to aquomethemoglobin and subsequently to hemichrome without crystal cracking. The rate of aging appears to be the same for both types. The hemoglobin crystal modification observed in the digestive system ofRhodnius prolixus is highly host- but not parasite- specific. The same modification is also observed in vitro and inOrnithodorus moubata, an arachnid whose digestive system differs considerably from that of the insectRhodnius. The retainment period of the crystals represents a long term host-record of possible medical interest.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01990090
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  • 6
    Electronic Resource
    Electronic Resource
    High pH-values and secretion of ions on leaf surfaces: A characteristic of the phylloplane of Malvaceae (1984)
    Springer
    Cellular and molecular life sciences 40 (1984), S. 935-937 
    Details
    ISSN: 1420-9071
    Keywords: Ion secretion ; leaf surface pH ; Malvaceae ; phylloplane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary All of 19 different species of Malvaceae have high pH-values, pH 9.5 or higher, on one or both surfaces of mature leaves. This is due to the secretion of magnesium and potassium carbonate and bicarbonate. The unusual pH and ionic composition may be important for parasites and pathogens living in the phylloplane of Malvaceae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01946445
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  • 7
    Electronic Resource
    Electronic Resource
    Surface characteristics of oocytes from juvenile mice as observed in the scanning electron microscope (1980)
    Springer
    Anatomy and embryology 161 (1980), S. 19-27 
    Details
    ISSN: 1432-0568
    Keywords: Surface characteristics ; Oocytes ; Mouse ; Scanning Electron Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The in vivo and in vitro development of the zona pellucida as well as the vitelline membrane surface of oocytes isolated from juvenile mice aged between 8 and 30 days p.p. were investigated by scanning electron microscopy. In vivo a consistent development of the zona pellucida surface can be observed, namely the formation of a fibrous network like structure interspersed with numerous pores. After enzymatic removal of the zona pellucida the surface of the vitelline membrane was studied. In “normal” oocytes with intact germinal vesicles, microvilli were distributed over the entire oocyte surface in all age groups investigated. After resumption of meiosis in vitro a characteristic differentiation on the vitelline membrane occurs. A glabrous polar region appears in primary oocytes and a glabrous polar body in secondary oocytes. The same differentiation in surface organization could be observed in the in vivo precociously matured oocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304666
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  • 8
    Electronic Resource
    Electronic Resource
    Cytogenetic investigations on development and degeneration of oocytes from juvenile mice (1980)
    Springer
    Anatomy and embryology 161 (1980), S. 9-18 
    Details
    ISSN: 1432-0568
    Keywords: Cytogenetics ; Oocytes ; Development ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Oocytes were isolated by mechanical disruption of the ovarian tissue of juvenile mice aged between 8 and 28 days p.p. Different types of oocytes could be distinguished by direct observation using an inverted light microscope. Their relative amount within the oocyte population isolated was determined in relation to age of the animals. The following types were isolated and examined: 1. “Normal” oocytes containing an intact germinal vesicle (that is, a nucleus in dictyate stage). In all age groups 55 to 83% of the oocytes were of this type. Their nuclear developmental stage was determined by studying their capacity to resume meiosis in culture as a function of age. 45% of the oocytes isolated from 15 day old mice were competent to resume meiosis. In mice aged 21 days more than 80% of the oocytes measuring about 80 μm in diameter resumed meiosis in vitro. No further increase in mean diameter or in the amount of competent oocytes could be found in older mice. 2. Degenerating oocytes. Five different types could be distinguished. a) “Atretic” oocytes present in all age groups investigated with pyknotic or disintegrating nuclei; b) “Precociously matured” oocytes first observed in mice aged 15 days containing normal diakinesis-and metaphase II-chromosomes; c) “Vacuolized” oocytes first isolated on day 18 having metaphase II-chromosomes scattered within the cytoplasm; d) “Fragmented” oocytes also first observed 18 days p.p. containing chromatin dissociated into micronuclei of different sizes within the cytoplasmic fragements; e) “Ghost” oocytes present in all age groups, characterized by the disintegration of the cytoplasm so that only the surrounding zona pellucida can be clearly seen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304665
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  • 9
    Electronic Resource
    Electronic Resource
    Long-term cultivation of plasma cell leukemia cells and autologous lymphoblasts (LCL) in vitro: A comparative study (1978)
    Springer
    Annals of hematology 36 (1978), S. 331-338 
    Details
    ISSN: 1432-0584
    Keywords: Plasma cell leukemia ; LCL ; EBV
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two long-term cell lines were established in vitro from the peripheral blood of a patient with plasma cell leukemia: one line with plasma cell proliferation, the other with lymphoblastoid cell proliferation (LCL). The 9-month-old plasma cell line showed the typical morphology of plasmoblasts. The cells neither had B-nor T-lymphocyte characteristics, were EBV negative, and showed aneuploidy with various marker chromosomes, including the 14 q+ marker. The cytogenetic findings indicate a monoclonal proliferation of the plasmacells. No tumor growth in thymusless nude mice could be induced upon intracranial inoculation with these cells. In contrast, the autologous LCL, cultured after addition of exogenous EBV, showed the characteristic markers of lymphoblastoid cells, with the typical morphology of pear and handmirror-shaped lymphoblasts, growing in clumps. They had C3- and Fc-receptors, surface-Ig, E-rosette-negativity, a diploid karyotype, and EBV dependent macromolecule synthesis. The lymphoblastoid cells produced intracranial tumors in nude mice in 8 out of 8 attempts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01000590
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  • 10
    Electronic Resource
    Electronic Resource
    Rasterelektronenmikroskopische Befunde an Netzhautinnenflächen (1976)
    Springer
    Graefe's archive for clinical and experimental ophthalmology 201 (1976), S. 29-38 
    Details
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es werden die rasterelektronenmikroskopischen Befunde an drei Netzhautrundlöchern beschrieben. Man findet eine aus den Foramina auf die Netzhautinnenfläche auswachsende Gliamembran mit mikrovillöser Oberfläche. Diese Zellmembran schiebt sich auf einen Kollagenfaserfilz der umgebenden vitreoretinalen Grenzschicht. Rudimente von Sinneszellrezeptoren stehen an den tiefen Lochrändern. Die Schicht der Pigmentepithelien unter den Foramina ist durch exzessiven Microvillibesatz ausgezeichnet.
    Notes: Summary The findings by scanning electron microscopy on three retinal holes are described. An outgrow of a glial membrane with microvilli from the holes to the retinal surface can be seen. This epiretinal cell membrane grows on a felt of collagen fibres of the vitreoretinal borderlayer. Rudiments of visual receptors project from the deep walls into the holes. The layer of the pigment epithelial cells beneath the holes is remarkable by the abundance of microvilli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410145
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