Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 1975-1979  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Nucleic acid metabolism in yeast (1975)
    Springer
    Molecular genetics and genomics 143 (1975), S. 113-118 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The three haploid yeast strains T2tmp1-3, T2tmp1-1, and T6tmp1-51 auxotrophic for 5′-dTMP differ in their requirement for thymidylate: 72, 16, and 3 μg 5′-dTMP/ml will restore optimal growth, respectively. Thymidylate low requirement in strain T2tmp1-1 and T6tmp1-51 is termed tlrA and tlrC, respectively. When the growth medium is made 5x10-4 M for 5′-dTMP only strain T6tmp1-51 is severely inhibited in RNA and DNA synthesis. This inhibition is reversible after removal of excessive 5′-dTMP. The inhibitory characteristic is in marked contrast to “thymineless death” due to the lack of 5′-dTMP in strain T6tmp1-51 where only DNA synthesis stops while RNA synthesis continues. The inhibitory effect of 5x10-4 M 5′-dTMP is not due to the 5′-dTMP auxotrophy but to the thymidylate low requiring character (tlrC) in strain T6tmp1-51. The arrest of RNA and DNA synthesis by high concentrations of exogenous 5′-dTMP suggests a regulatory role of either the monoor triphosphate on nucleoside or nucleotide biosynthesis in yeast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269428
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Formation and fate of cross-links induced by polyfunctional anticancer drugs in yeast (1979)
    Springer
    Molecular genetics and genomics 176 (1979), S. 41-52 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method to detect low levels of interstrand cross-links in DNA of Saccharomyces cerevisiae is described. Isopycnic ultracentrifugation of alkali-treated, unpurified Eaton press homogenates allows the detection of less than one cross-link per yeast chromosome. Efficient separation of single-and double-stranded DNA requires low cell density and addition of glycerol during homogenization. Using a yeast strain defective in excision repair, a dose dependent formation of interstrand cross-links after treatment of cells with biological doses of nitrogen mustard. Triaziquone and Chloramubil could be demonstrated. The most powerful of these alkylating agents is Triaziquone: half of the DNA molecules are shown to be cross-linked after a 12 min exposure to 9×10-9 g/ml of the drug. The cross-linking reaction continues after excessive alkylating agent is removed. After having reached a maximum the fraction continues after excessive alkylating agent is removed. After having reached a maximum the fraction of renaturable DNA decreases upon further incubation. The speed of this “after-reaction” depends on temperature: 48 h after the end of treatment renaturability of DNA has almost completely disappeared when cells are kept at 36° C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334294
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    A simple method for the isolation and characterization of thymidylate uptaking mutants in Saccharomyces cerevisiae (1976)
    Springer
    Molecular genetics and genomics 147 (1976), S. 209-215 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mutant tmp1−10 ts which confers thermosensitive auxotrophy for thymidylate is employed for the selection of 5′-dTMP uptaking mutants. At the nonpermissive temperature yeast cells phenotypically wild type for thymidylate uptake can grow for only 3 to 4 generations in the presence of 10−2 M 5′-dTMP. Thymidylate utilizing mutants (tum mutants) were isolated which can grow in the presence of 12 to 24 μg 5′-dTMP/ml. Genetical analysis revealed one of these mutant strains to be a double mutant, tuml tum2. For normal growth haploid thymidylate auxotrophic strains require approximately 360 μg 5′-dTMP/ml when tum1 and 24 μg 5′-dTMP when tum2 is present, respectively. Cells prototrophic for thymidylate (TMP) harbouring tum1 tum2 will also take up 5′-dTMP and incorporate it specifically into their DNA. Thymidylate utilization in such strains is independent of functional mitochondria, as similar incorporation of labelled 5′-dTMP is found in isogenic strains with rho +, rho − and rho 0 status. Optimal stimulation of the 5′-dTMP uptaking principle in haploid TMP strains is found at 4 μg 5′-dTMP/ml when tum1 and tum2 are present.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267573
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...