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  • 1
    Electronic Resource
    Electronic Resource
    THE CYTOCHEMICAL BIOASSAY OF CORTICOTROPIN (ACTH) (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 297 (1977), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1977.tb41857.x
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  • 2
    Electronic Resource
    Electronic Resource
    Dose-dependent cytochemical responses of the guinea pig renal cortex to femtogram quantities of bovine parathyroid hormone (1976)
    Springer
    Calcified tissue international 22 (1976), S. 385-385 
    Details
    ISSN: 1432-0827
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02064107
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Pentose-shunt oxidation in the periosteal cells in healing fractures (1977)
    Springer
    Calcified tissue international 23 (1977), S. 77-81 
    Details
    ISSN: 1432-0827
    Keywords: Fracture Healing ; Glucose 6-phosphate ; Dehydrogenase ; Glutathione ; Microdensitometry ; Cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The activity of pentose-shunt dehydrogenases is very low in periosteal cells of normal rat metatarsals, but increases one day post-fracture and rises linearly over the next two days. By four days postfracture, the distribution of this activity along the bone shows two centres of high activity: the first in the region of proliferation to form callus and the second at the site where new bone is first seen, one day later. The high rate of generation of NADPH would be expected to reduce glutathione; reduced glutathione has been shown to inhibit alkaline phosphatase activity in these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02012769
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Changes in alkaline phosphatase activity in periosteal cells in healing fractures (1977)
    Springer
    Calcified tissue international 22 (1977), S. 19-25 
    Details
    ISSN: 1432-0827
    Keywords: Fracture ; Alkaline phosphatase ; Microdensitometry ; Undemineralised sections
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract The quantitative changes in alkaline phosphatase activity in the periosteal cells close to the fracture in rat metatarsal bones has been measured during the first 5 days postfracture. This study has been made possible by two technological advances, firstly the development of cryostat microtomy for cutting unfixed, undemineralised bone, and secondly the use of scanning and integrating microdensitometry for quantifying the activity in each periosteal cell. The results showed a loss of alkaline phosphatase activity close to the fracture site, with activity rising to normal values 0.8–1.0 mm from the site. No alkaline phosphatase activity was found in the cells which proliferated from the periosteum. It is suggested that reduced glutathione could cause such inhibition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02010342
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  • 5
    Electronic Resource
    Electronic Resource
    The direct measurement of cytochrome P450 in unfixed tissue sections (1975)
    Springer
    Histochemistry and cell biology 41 (1975), S. 227-232 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A method for the measurement of cytochrome P450 in unfixed cryostat sections is described. The sections are incubated for 10 minutes at room temperature in a buffered solution containing polyvinyl alcohol and sodium dithionite. Two incubations are performed on serial sections, one in nitrogen and the other in carbon monoxide. Readings are taken on a Vickers M85 microdensitometer fitted with a high sensitivity photomultiplier amplifier system, the measurements being made on corresponding fields in the serial sections. Subtraction of the nitrogen values from the carbon monoxide values, after allowing for an absorption shift, gives the absolute spectrum of cytochrome P450. The subtraction corrects for the tissue content of other haem-containing proteins. The cytochrome P450 spectrum shows a sharp maximum at 450 nm, and two other minor components absorbing at 444 nm and 458 nm. The content of cytochrome P450 in animals fed with phenobarbitone was 2.4 times greater than in control animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00497685
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  • 6
    Electronic Resource
    Electronic Resource
    The effect of thyrotrophin on oxidative activity in thyroid follicle cells (1975)
    Springer
    Histochemistry and cell biology 41 (1975), S. 323-334 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Biochemical evidence, based on supra-physiological concentrations of thyrotrophin (TSH), has indicated that this hormone increases the NADP concentration in thyroid follicle cells. The hormone is believed to act both to enhance the reoxidation of the reduced coenzyme and to generate NADP from NAD. The latter effect, mediated by NAD+-kinase, could be a major control mechanism in thyroid metabolism. In the present study increased sensitivity has been obtained by the use of maintenance culture and of microdensitometry for measuring enzyme activity in the follicle cells. Using low physiological (0.1 μU/ml), and sub-physiological concentrations of the hormone as are used in the cytochemical bioassay systems, it has been shown that the rate of reoxidation of NADPH is enhanced by TSH. Moreover the NAD+-kinase activity is also greatly increased, and shows a direct relationship to the concentration of the hormone acting on the segments of the gland in vitro. It is possible that this phenomenon could be used to assay TSH. The results indicate that NAD+-kinase activity may play a significant part in the control of pentose-shunt oxidation in thyroid follicle cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00490075
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    Paper (German National Licenses)
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