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Ultrastructural changes of the early visceral yolk sac layer of mouse embryos after maternal injection of trypan blue (1978)

Schlüter, G.

Springer

Anatomy and embryology 153 (1978), S. 287-293

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ISSN: 1432-0568

Keywords: Mouse embryos ; Visceral yolk sac ; Trypan blue ; Electron microscopy

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary The ultrastructural features of the early visceral yolk sac epithelium of normal mouse embryos on day 9 were compared to those whose mothers had received a single subcutaneous injection of 100 mg/kg trypan blue on day 8. The following results were obtained: In normal embryos the visceral yolk sac cells are predominantly characterized by numerous membrane bounded inclusions localized in the supranuclear cytoplasm. In embryos of animals treated with trypan blue, at about 12h after injection large single and only partly membrane bounded vacuoles are observed occupying most of the apical cytoplasm. Up to 24h after injection large cytoplasmic areas are seen which are in a stage of autodigestion possibly due to leakage of the vacuolar content. These alterations are exclusively limited to the visceral yolk sac epithelium whereas in the cells of the embryonic part, e.g. head process, no changes could be found. The observations are discussed in relation to the mechanism of the teratogenic activity of trypan blue.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00315931

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Mikroverfahren für Refraktometrie in Capillarküvetten (1975)

Sernetz, M. ; Schlüter, G. ; Ostwald, M.

Springer

Fresenius' Zeitschrift für analytische Chemie 273 (1975), S. 97-100

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ISSN: 1618-2650

Keywords: Refraktometrie ; Mikroverfahren in Capillarküvetten

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Description / Table of Contents: Zusammenfassung Das beschriebene Mikroverfahren gestattet es, in Capillaren interferometrisch Refraktometrie an Flüssigkeiten mit Probevolumina von 10−10 1 durchzuführen. Die Eignung der Capillaren als optische Küvetten auch für andere mikroanalytische photometrische Verfahren wird diskutiert.

Notes: Abstract The micromethod described allows refractometry of fluids in sample volumes as low as 10−10 1 by means of interferometry in capillaries. The suitability of the capillaries as optical cuvettes for other microphotometric analyses is discussed.