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  • 1975-1979  (7)
Source
Material
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Removal and Refixation of Surface Ig from Isolated Rat Mast Cells (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 6 (1977), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This study showed that under well-defined conditions all immunoglobulins could be removed from the rat mast cell surface. Surface immunoglobulins were examined by immunofluorescence technique, and cell function by the allergic reaction to antigen as judged by histamine release. Refixation of eluted surface Ig to the mast cell was easily accomplished. Furthermore, fixation of specific surface Ig to already sensitized cells resulted in inreased cell sensitivity to antigen, whereas fixation of nonspecific surface Ig resulted in decreased sensitivity. The results indicate that removed immunoglobulins are intact, have affinity for mast cells, and are able to compete with cell-bound Ig.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1977.tb00337.x
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    3H-Histamine Release from Human Leukocytes (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 34 (1979), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A rapid, simple, and inexpensive method for large scale screening of patients suspected of type I allergy has been developed. The method is based on in vitro incorporation of 3H-histamine in the leukocytes of the patient, whereafter release of labelled histamine is measured after provocation of the cells with the suspected allergen. The new method was compared with the conventional basophil histamine release technique by in vitro provocation of six asthmatic patients under suspicion of type 1 allergy against animal dander, house dust, and mite, and an almost identical it-lease of histamine was observed in both assays.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1979.tb01707.x
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    A SIMPLIFIED METHOD FOR MEASURING BASOPHIL HISTAMINE RELEASE AND BLOCKING ANTIBODIES IN HAY FEVER PATIENTS. BASOPHIL HISTAMINE CONTENT AND CELL PRESERVATION (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 32 (1977), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1977.tb01347.x
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Binding of Antigen to Surface Ig during in vitro Hyposensitization of Isolated Rat Mast Cells (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 34 (1979), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The mechanism of in vitro hyposensitization was examined in isolated rat mast cells. Surface distribution of immunoglobulin was examined by immunofluorescence technique. Hyposensitization could be ascribed neither to changes in the distribution of surface immunoglobulins nor to capping or shedding phenomena. The distribution of cell-bound antigen and the number of antigen-positive cells (patch-bearing cells) was not changed by hyposensitization. The binding of antigen to surface Ig was quantitated by I125-labelled antigen. In rats sensitized to two different antigens, hyposensitization of the mast cells with one of these inhibited the subsequent histamine release induced by either of the two antigens, whereas no changes were observed in the antigen binding capacity.The present investigation indicates that the mechanism of in vitro hyposensitization is the result of events secondary to the initial binding of antigen to the mast cell surface immunoglobulin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1979.tb01999.x
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    CYCLIC AMP AND ALLERGIC HISTAMINE RELEASE. INFLUENCE OF METHYLXANTHINES ON RAT MAST CELLS (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 32 (1977), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1977.tb01348.x
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Quantitative and Qualitative Estimations of IgE Bound to Basophil Leukocytes from Hay Fever Patients (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 6 (1977), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: IgE was removed from human basophils of 4 nonatopic persons and 10 hay fever patients allergic to timothy grass pollen by treating the cells with buffer to adjusted pH 4. IgE could be removed and refixed to the same cells. Refixation was demonstrated by immunofluorescence and by the ability of basophils to release histamine on exposure to timothy pollen. Removed total IgE and specific IgE directed against timothy pollen were estimated, and a linear correlation to the level of total IgE and specific IgE in serum was found. The total number of IgE molecules per basophil was calculated to be in the range of 30,000 to 300,000, and timothy-specific IgE constituted 4%-15% of the total IgE molecules on the cells. It was furthermore established that specific cell-bound IgE was linearly correlated to the pollen concentration releasing 20% of the histamine contents of the basophils. Separated IgE from sensitized and nonsensitized basophils could be bound to basophils from other patients, resulting in a change in cell sensitivity. This could be ascribed to additional binding to free cell receptors as well as to a partial replacement of bound IgE. Basophils from nonatopic persons could not be sensitized by incubation with surface IgE from atopic persons. The results indicate that acid treatment is a simple method suitable for removing IgE from basophils. This IgE is intact and can be quantitated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1977.tb00338.x
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Allergic reactions, cyclic AMP and histamine release (1977)
    Springer
    Cellular and molecular life sciences 33 (1977), S. 965-966 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Both isobutylmethylxantine and theophylline increased the level of cyclic AMP in the mast cell. Theophylline reduced the allergic histamine release, whereas isobutylmethylxynthine caused a pronounced potentiation of the histamine release. This indicates that the hypothesis of an inverse relationship between the level of cyclic AMP in mast cells and histamine release is too simple.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01951308
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    Paper (German National Licenses)
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