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  • Articles: DFG German National Licenses  (5)
  • 1970-1974  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Intracellular study of frog's vestibular neurons in relation to the labyrinth and spinal cord (1974)
    Springer
    Experimental brain research 19 (1974), S. 377-393 
    Details
    ISSN: 1432-1106
    Keywords: Frog vestibular neurons ; Dendritic spikes ; Vestibular efferents ; Electrotonic coupling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Field and intracellular potentials were recorded in the vestibular nuclei of the frog following stimulation of the anterior branch of the ipsilateral vestibular nerve and the spinal cord. The field potential induced by stimulation of the vestibular nerve consisted of an early positive-negative wave followed by a slow negativity and that recorded during spinal cord stimulation was composed of an antidromic potential followed by a slow negative wave. These potentials were most prominent in the ventral region of the stato-acoustic complex. Mono- and polysynaptic EPSPs were recorded from vestibular neurons following vestibular nerve stimulation. Short latency depolarizations of small amplitude preceded the monosynaptic EPSPs in some neurons. Spike-like partial responses were commonly superimposed on the EPSPs. These all-or-none depolarizations probably originated in the dendrites. In a group of vestibular neurons stimulation of the vestibular nerve evoked full action potentials with latencies ranging from 0.2 to 1.1 msec. They are presumably caused by antidromic activation of neurons which send their axons to the labyrinth. The presence of efferent neurons in the vestibular nuclei was confirmed by their successful staining with Procion Yellow following axonal electrophoresis. After stimulation of the spinal cord, antidromic spike potentials and EPSPs were recorded in vestibular neurons. In addition, short-latency depolarizing potentials (EDPs) were evoked by spinal stimulation, with latencies similar to those of antidromic potentials. The EDPs are suggested to be induced by electrotonic transmission from the neighboring cell and likely to be active spike potentials produced at some distance away from the soma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234462
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Monosynaptic excitatory and inhibitory pathways from medial midbrain nuclei to trochlear motoneurons (1974)
    Springer
    Experimental brain research 20 (1974), S. 223-238 
    Details
    ISSN: 1432-1106
    Keywords: Trochlear nucleus ; Interstitial nucleus of Cajal ; Darkschewitsch nucleus ; Descending oculomotor control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Stimulation of both an ipsi and contralateral medial midbrain area in pentobarbital anesthetized cats evoked an EPSP-IPSP sequence in trochlear motoneurons (TMn). The latencies of both the EPSP and IPSP were in the monosynaptic range. Experiments on chronic medial longitudinal fasciculus sectioned cats showed that the TMn effects were not produced by collaterals of ascending vestibular axons antidromically excited from the medial midbrain. Stimulation of the same area had no effect in abducens motoneurons, but produced EPSPs and spikes in reticular neurons just ventral to the VI nucleus. The effective area for producing TMn PSPs, as delimited by multiple stimulation electrode arrays, extended only over the interstitial nucleus of Cajal-nucleus of Darkschewitsch area (IN). The effective bilateral IN areas appeared homogeneous in their ability to produce both the EPSP and IPSP components in TMns, even at minimum stimulus strength. It was concluded that the IN area is the origin of monosynaptic excitatory and inhibitory pathways to TN, and it was discussed how the IN has the synaptology required of an integration center for reciprocal control of conjugate vertical and rotational eye movements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00238314
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Coupling of Polysaccharides to Erythrocytes by Cyanogen Bromide (1974)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 3 (1974), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A simple procedure for covalent coupling of polysaccharides (PS) to erythrocytes for passive hemagglutination is described. The PS is dissolved in saline and activated by adding cyanogen bromide, keeping the pH at 10.7. After adjusting the pH to 7.8, the activated PS solution is admixed to a 2% erythrocyte suspension for coupling, for 2 hr. Using dextran fractions for coupling, such sensitized cells show a high degree of specific sensitivity, similar to that produced by contact with stearoyl-dextran. Another PS, guaran, was similarly coupled to erythrocytes, conferring on them a high degree of specific sensitivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1974.tb01266.x
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Extended survival of the chick embryo in vitro (1973)
    Springer
    Cellular and molecular life sciences 29 (1973), S. 467-468 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé On décrit une technique pour obtenir la survie prolongée des embryons du poulet in vitro, et son emploie pour cultiver des tissus isolés du système nerveux centrale.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01926786
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Vestibulospinal effects on hindlimb motoneurons of the frog (1974)
    Springer
    Pflügers Archiv 348 (1974), S. 211-223 
    Details
    ISSN: 1432-2013
    Keywords: Frog-Vestibulospinal System ; Spinal Motoneurons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Field and intracellular potentials were recorded in the lumbar spinal cord of the frog following stimulation of the anterior branch of the vestibular nerve and vestibular nucleus. The field potential recorded in the motoneuron pool after VIIIth nerve stimulation consisted of two presynaptic positive-negative potentials (latencies 1.7 and 2.6 msec) followed by a slow negative wave. The latency of the first presynaptic field potential was only 0.6 msec longer than the presynaptic field potential evoked by stimulation of the vestibular nucleus; it is suggested that electrotonic coupling in the vestibular nuclei is responsible for the fast vestibulospinal transmission. Whereas VIIIth nerve stimulation produced EPSPs in both flexor (peroneal) and extensor (tibial) motoneurons, IPSPs were found only in extensor motoneurons. The functional implication of these findings was discussed. Comparison of PSP latencies with the extracellular presynaptic field potentials generated by VIIIth nerve or nucleus stimulation indicated that EPSPs were produced by the excitatory action of vestibulospinal axons on motoneurons. The longer latencies of the vestibular induced IPSPs suggested that they were generated indirectly by inhibitory spinal interneurons. Preliminary experiments on the interaction of segmental and vestibular induced PSPs suggest that the latter are generated close to the soma of motoneurons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00587412
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    Paper (German National Licenses)
    Fulltext
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