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  • 1970-1974  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 6 (1971), S. 0 
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Using strain gauges, the method to be described measures the lateral force required to produce a standard deflection of toothbrush tufts. A deflector bar which remains stationary is attached to a cantilever which is stressed by the pressure of the brush moving across the deflector bar. The resulting strain is measured by using strain gauges which are connected via a Wheatstone bridge to an oscilloscope. Movements of the oscilloscope beam represent changes in the force exerted by the brush and are photographed on moving film.The effects on stiffness of changes in the rate of strain, length, diameter, wetness and temperature of nylon were investigated separately, and a standard test was devised which could assist in more accurate labelling of toothbrushes.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The preparation of an antiserum to a purified suspension of adenovirus type 5 hexons is described. The antiserum reacts by complement fixation tests with type 5 and with many other adenoviruses. The anti-hexon serum can be used for the immunofluorescent detection of the site of hexon synthesis within tissue culture cells infected with many different adenoviruses, and therefore allows diagnostic detection of the adenovirus group antigen. In experimentally infected cultures, “group” identification could be made within 24 hours of infection when many different adenoviruses were tested.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A method is described for the removal of contaminating (endogenous) protein from high titre virus suspension. The method depends upon adsorption of the virus to barium sulphate suspension at 37° C and its subsequent elution at 4° C into sodium citrate solution. The efficiency of protein removal varied from 90 to 98.9%, corresponding to a fall from 1400 to 150 μg/ml, and from 9500 to 110 μg/ml with Coxsackie B 4 and mumps viruses, respectively. Other viruses, for which intermediate figures were obtained included several myxoviruses, vaccinia, herpes simplex and respiratory syncytial virus. Subsequent immunization of guinea-pigs with ‘protein-reduced’ virus suspensions confirmed the retention of viral immunogenicity after treatment in terms of complement-fixing, neutralizing and immunofluorescent antibody titres induced. All the treated viruses except respiratory syncytial virus produced an antiserum in guinea-pigs completely free from non-specific fluorescent when used without dilution in a homologous immunofluorescent test system.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A method is described for the construction of five intersecting pools of guineapig antiserum to be used in the immunofluorescent identification of eight common respiratory viruses. The component antisera are highly purified and produce no non-specific fluorescence at working titre before or after incorporation into pools. The antiserum pools are fully stable at 4°C for up to 2 weeks, and for at least 3 months when stored at −20° C. Due to their intersecting nature, a direct “read-out” of the type of virus present is possible. The pool system also offers the advantage of built-in negative controls for each specimen examined.
    Type of Medium: Electronic Resource
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