ISSN:
1432-8798
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary A method is described for the removal of contaminating (endogenous) protein from high titre virus suspension. The method depends upon adsorption of the virus to barium sulphate suspension at 37° C and its subsequent elution at 4° C into sodium citrate solution. The efficiency of protein removal varied from 90 to 98.9%, corresponding to a fall from 1400 to 150 μg/ml, and from 9500 to 110 μg/ml with Coxsackie B 4 and mumps viruses, respectively. Other viruses, for which intermediate figures were obtained included several myxoviruses, vaccinia, herpes simplex and respiratory syncytial virus. Subsequent immunization of guinea-pigs with ‘protein-reduced’ virus suspensions confirmed the retention of viral immunogenicity after treatment in terms of complement-fixing, neutralizing and immunofluorescent antibody titres induced. All the treated viruses except respiratory syncytial virus produced an antiserum in guinea-pigs completely free from non-specific fluorescent when used without dilution in a homologous immunofluorescent test system.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01242981
