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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of virology 32 (1970), S. 147-156 
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Using the indirect immuno-ferritin technique virus specific antigen can be detected in L cells infected with Mengo-L virus 8–16 hours after infection. Most of the viral protein is localized in the cytoplasm. Aggregates of ferritin particles showing a diameter of about 40 mμ indicate single virus particles. The surface of both, numerous vesicles and the nucleus membrane is heavily tagged with viral antigen. Vesicles coated with viral protein are identified as typical fluorescent granules as revealed by the immunofluorescent technique. Crystalline arrays of virus particles are absent. Some individual ferritin particles are seen within the nucleus.
    Notes: Zusammenfassung Mit Hilfe der indirekten Immunferritintechnik kann in Mengovirus-infizierten L-Zellen 8 bis 16 Stundenp.i. virusspezifisches Protein nachgewiesen werden. Die überwiegende Menge des Virusantigens befindet sich im Zytoplasma. Aggregate von Ferritinpartikeln mit einem Durchmesser von etwa 40 mμ zeigen einzelne Virusteilchen an. Die Oberfläche der zahlreichen Vesikeln sowie der Kernmembran ist von virusspezifischem Protein besetzt. Die von Virusprotein umgebenen Vesikeln sind mit den fluoreszenzserologisch darstellbaren Granula identisch. Viruskristalle wurden nicht gefunden. Der Kern enthält einzelne Ferritinpartikel.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of virology 33 (1971), S. 49-60 
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Actidione (0.15 μg/ml) significantly inhibits the multiplication of Mengo virus in L cells when present in the period from 1 1/2 to at least 5 hours after t0. The number of cells synthesizing structural proteins is reduced by about 95% when the antibiotic is present from about 1 1/2 to 4 hours after t0 as revealed by immunofluorescent studies. Viral antigen is produced only in a few cells and the fluorescent material mostly is distributed homogeneously. The typical fluorescent granules appear rarely. From the results it can be concluded that actidione inhibits synthesis of virus protein by interfering with the synthesis of both early and structural proteins. It is assumed that the structural proteins synthesized in the presence of actidione are impaired in virus assembly.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    International archives of occupational and environmental health 27 (1971), S. 338-348 
    ISSN: 1432-1246
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Von 88 Kranken mit einer chronischen Bronchitis atmeten 25 fÜr 15 min und 63 fÜr 30 AtemzÜge ein NO2-Luft-Gemisch mit NO2-Konzentrationen zwischen 0,5 und 5,0 ppm. Vor und nach der Inhalation wurden folgende Grö\en untersucht: 1. Atemfrequenz (f), Atemminutenvolumen ( $$\dot V_E $$ ), Atemvolumen (VT) und Sauerstoffaufnahme ( $$\dot V_{O_2 } $$ ). 2. Arterieller Sauerstoff- und Kohlensäurepartialdruck ( $$Pa_{O_2 } $$ und $$Pa_{CO_2 } $$ ) und die Wasserstoffionenkonzentration im arteriellen Blut (pHa). 3. Die endexspiratorischen Gasdrucke von Sauerstoff und Kohlensäure ( $$P_{A_{O_2 } } $$ und $$P_{A_{CO_2 } } $$ ) und die endexspiratorisch-arterielle Sauerstoff- und Kohlensäuredruckdifferenz (AaD-O2 und aAD-CO2). 4. Die Strömungswiderstände in den Atemwegen (Rt). Als wesentliches Ergebnis stellte sich heraus, da\ 1. bei unverändertem $$P_{A_{O_2 } } $$ nach Inhalation von 5 und 4 ppm NO2 $$Pa_{O_2 } $$ signifikant abnahm und die AaD-O2 entsprechend zunahm. Nach Inhalation von 2 ppm NO2 lie\ sich ein Abfall des $$Pa_{O_2 } $$ nicht mehr nachweisen. 2. Die Strömungswiderstände in den Atemwegen nahmen nach Inhalation von NO2-Konzentrationen zwischen 1,5 und 2,0 ppm noch signifikant zu.
    Notes: Summary 88 patients with chronic bronchitis breathed an NO2-air mixture containing 0,5 to 5.0 ppm NO2 for 15 minutes or a total of 30 breaths. Before and after the inhalation the following measurements were performed: 1. tidal volume (VT), minute-volume ( $$\dot V_E $$ ), frequency (f) and O2-uptake ( $$\dot V_{O_2 } $$ ). 2. arterial gas pressures ( $$Pa_{O_2 } $$ and $$Pa_{CO_2 } $$ ) and pHa. 3. the endexpiratory gas pressures ( $$P_{A_{O_2 } } $$ and $$P_{A_{CO_2 } } $$ ) and the endexpiratory-arterial pressure differences (AaD-O2 and aAD-CO2). 4. airway-resistance (Rt). The most important findings were: 1. while $$P_{A_{O_2 } } $$ remained nearly constant during inhalation of 5 and 4 ppm NO2, a significant decrease of the $$Pa_{O_2 } $$ and accordingly an increase of AaD-O2 occured. After inhalation of 2 ppm NO2 there was no decrease of $$Pa_{O_2 } $$ . 2. After inhalation of NO2-concentrations down to 1.5 ppm, the airway resistance still increased significantly. Lower concentrations had no significant effect.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    International archives of occupational and environmental health 31 (1973), S. 61-72 
    ISSN: 1432-1246
    Keywords: NO2 effects ; Diffusion ; Distribution of perfusion ; Resistance ; Scintigraphy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The acute influence of NO2 in MAC value concentrations upon the pulmonary function was studied in 55 healthy male subjects (18–65 years old) and 84 male patients suffering from chronic bronchitis (30–72 years old). The latter were admitted to the hospital because of an exacerbation of their disease. Additionally, the distribution of lung perfusion before and after inhalation of NO2 concentrations between 30 and 50 ppm was determined by scintigraphy in rabbits. These studies resulted in the following: 1. An inhalation of 5 ppm NO2 over 15 min led to a significant decrease of the CO diffusing capacity. In 16 healthy subjects, the mean decrease of D LCOwas by 3.8 ml · min−1 · torr−1 (p〈0.01). 2. A prolongation of the exposure period in chronic bronchitics from 15 to 60 min at a NO2 concentration of 5 ppm did not result in a disturbance of the respiratory gas exchange for oxygen beyond the extent of the disturbance observed after exposure to 5 ppm NO2 over 15 min (p〉0.1). 3. For the concentration range up to 5 ppm, a relationship between the inhaled concentration of NO2 and the increase of airway resistance was obvious; no effect seemed to be in concentrations 〈1.5 ppm. 4. No correlation was found between the increase of R tafter the inhalation of NO2 as dependent upon the initial value of airway resistance. 5. In the lungs of the rabbit, scintigraphy revealed that an inhalation of 30 ppm NO2 over 15 min resulted in a re-distribution of perfusion showing a reduced storage of activity in the peripheral areas of the lungs. These findings are discussed with a view to a causative association between air pollution, inhalation of tobacco smoke, chronic bronchitis, and pulmonary emphysema. Taking into account experimental studies in animals, the authors feel that the impairment of pulmonary functions observed after acute inhalation of low concentrations of NO2 if frequently repeated over a number of years may have, together with other causative factors, an influence upon the development and maintenance of chronic bronchitis including pulmonary emphysema.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Archives of virology 42 (1973), S. 144-153 
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Sindbis virus has been adapted to L cells in the course of serial passages until titre of infectivity and degree of cytopathic effect remained constant. The growth curve shows a latent period of 1 to 2 hours followed by an exponential rise of virus multiplication. Peak titre is reached 7 to 8 hours after infection. By means of immunofluorescence technique localization of the antigens of Sindbis virus in the infected cell was studied at various times after infection. Viral antigens are detectable exclusively in the cytoplasm. They appear as early as 1 hour after infection in a few cells, at first diffusely distributed, and beginning with the 4th hour, increasing in the form of fluorescent granules. These granules are supposed to represent accumulations of viral nucleocapsids at the surface of cytoplasmic vacuoles as well as mature virus within the vesicles as revealed by electron microscopic studies with several group A arboviruses (loc. cit.). Percentage of cells containing viral antigens reaches its maximum at 5 to 6 hours. At later stages of infection cells contain only a few big fluorescent aggregates.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Archives of virology 33 (1971), S. 41-48 
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of β-2-DL-thienylalanine on the antigen synthesis of Mengo virus in L cells was studied by microphotometric determination of immunofluorescence intensity by using single-cell and field measurement. It was found by both methods that intensity of fluorescence decreases significantly in the presence of the amino acid analogue (4.38 mM). However, it was shown that exact interpretations are possible only by using single-cell measurement.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 268 (1974), S. 245-249 
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A straightforward application of theU-matrix formalism is utilized to derive canonical exponential transformations. It turns out that an extremely compact form for these transformations can be written down. In view of the fact that the transformation used so far, mostly have been found by guessing, a simple calculation prescription for them seems to be of great value. As an application the derivation of the already wellknown transformations of theE-e andT-t Jahn Teller problems as well as that of the Fröhlich Hamiltonian is presented. In the Jahn-Teller examples it is further demonstrated, how for each single problem the validity region of the transformation may be examined.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 251 (1972), S. 300-313 
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The approach is based on the adiabatic principle and on a fundamental hierarchical postulate. The latter assumes that in the electronic ground-state the conduction-band contributions are small of first order for the chosen basic set of one-electron functions. From this linearized equations of motion beyond the Hartree-Fock approximation are established. Utilizing unitary transformations and Green's function techniques, the adiabatic potential for the lattice dynamics is calculated. It depends on the nuclear positions and on interband “shell-degrees of freedom”N αβ=〈a α + a β〉which are fixed by self-consistent subsidiary conditions. Transcription of the chosen quasi-Bloch-Basis to atomic quantities yields that for each transition α → β at the single ionmμ and for each “charge-transfer” excitation α1 → β2 between two ionsm 1μ1 andm 2μ2 there is one “shell degree of freedom”. By projection onto spherical harmonics and summation, integral shell modes of breathing, polarization and quadrupole-detormation may be introduced. It is realized that breathing and quadrupole deformation can be expected to have effects of comparable magnitude.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 256 (1972), S. 291-308 
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract In microscopic many-body physics the coupling between the motion of fast particles (electrons) and slow particles (nuclei) is universal. The standard Born-Oppenheimer decoupling procedure breaks down, if the energy separation in the “fast” system is of the same order as the elementary excitation in the “slow” system. In this case “dynamical resonance” effects are to be expected. In the present investigation a model system of a coupling between a doubly degenerate high energy excitation and doubly degenerate low energy oscillator is handled by a non-linear canonical transformation which is shown to be quasi-exact in the sense that it diagonalizes the Hamiltonian in both extremal coupling cases. The transformation has some flexibility, so that the diagonalization regions can be enlarged. It is employed to calculate the “zero-phonon” optical response, which indeed displays aresonance effect. Likewise, another nonlinear transformation is devised, which only in the strong coupling limit yields diagonalization. This latter transformation in a natural way leads to the conventional semi-classical approaches to the dynamical Jahn-Teller problem. The results gotten with it are identical with those from our transformation in the strong coupling limit. On the basis of our results some remarks are made concerning the possible impact of the breakdown of the adiabatic approximation in other regions.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 256 (1972), S. 22-42 
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract On the basis of quantum mechanical ab initio theories practical shell-models are established. These models include a polarization as well as a radial and axial deformation of the lattice ions, and are built up in such a way that measured macroscopic quantities can be incorporated. The predicted dynamics is tested by means of vibronic spectra of Sm2+ in SrF2 and BaF2. It turns out that both the structural form and the positions and intensities of the predominant vibronic maxima are very sensible to the high frequency dielectric constant ɛ∞. An excellent agreement with the experimental result is found if ɛ∞ fictitiously is chosen lower than the measured value, whereas the agreement would be considerably worse, if the experimental value itself of ɛ∞ is choosen. Possible sources of these discrepancies are discussed.
    Type of Medium: Electronic Resource
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