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  • 1
    Electronic Resource
    Electronic Resource
    Monospecific Polyclonal Anti-Anti-Idiotypic Antibodies to the Carboxyterminal Undecapeptide of the SV40 Large Tumour Antigen (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 41 (1995), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The murine monoclonal antibody PAb1605 defines an epitope, peptide Lys(698)-Thr(708) (KT), on the carboxyterminus of the tumour(T)antigen of SV40-transformed cells. In vivo and in vitro experiments had shown that this sequence represents an epitope for both humoral and cellular immune responses. When injected into rabbits PAb1605 induces anti-idiotypic antibodies (Ab-2). Ab-2β (internal image type) was purified by adsorption chromatography and characterized by the ability of KT to compete with the binding of ab-2 with ab-1. Murine anti-anti-idiotypic antibodies (ab-3) were obtained by immunization of mice with ab-2β. Both ab-1 and ab-3 JgG showed affinities to immunoprecipitated SV40 T antigen by immunoblot analysis and to nuclear SV40 T antigen by the immunofluorescence assay. The binding of ab-3 to SV40 T antigen was completely inhibited by competition with KT. We conclude that the polyclonal ab-3 is of the ab-3 subtype and specific for only one epitope which is represented by KT and defined by ab-1. The results demonstrate that the specificity for a defined peptide epitope of an antibody was conserved even after two consecutive steps of anti-idiotypic-antibody formation in two host species. Since this postulate of network theory could be verified for a sequence of a tumour-associated antigen which represents a B- and T cell epitope, this model is of great interest for further tumour immunological studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1995.tb03561.x
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  • 2
    Electronic Resource
    Electronic Resource
    Über die Präparation nephrotoxischer Antikörper in der Gamma-Globulinfraktion mittels der Cellulose-Ionen-Austausch-Chromatographie (1965)
    Springer
    Virchows Archiv 339 (1965), S. 308-315 
    Details
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Cellulose-ion-exchange chromatography is a technically simple procedure suited to elute rabbit gamma-globulin of a specific antibody character directed against rat renal tissue. With this procedure, the globulins preserve their specific antibody character. On i. v. injection into healthy rats these globulins are as capable of inducing a glomerulitis as i. v. administered socalled nephrotoxic whole serum.
    Notes: Zusammenfassung Die Cellulose-Ionen-Austausch-Chromatographie ist als ein technisch einfaches Verfahren geeignet, Gamma-Globuline von Kaninchen mit spezifischem Antikörpercharakter gegen Rattennierengewebe zu eluieren. Dabei scheinen diese Globuline ihren spezifischen Antikörpercharakter zu bewahren. Sie sind nämlich imstande, bei i.v. Injektion in gesunden Ratten ebenso eine Glomerulitis zu erzeugen wie i.v. einverleibtes sog. nephrotoxisches Vollserum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00959691
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  • 3
    Electronic Resource
    Electronic Resource
    Über Anwendungsmöglichkeiten der postmortalen Serumanalyse in der Obduktionsdiagnostik besonders bei Paraproteinosen (1965)
    Springer
    Virchows Archiv 339 (1965), S. 349-357 
    Details
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The reaction-constellation types 3, 5, 6, and 7 (afterWuhrmann andMärki) could be determined in the serum-proteins of cadavers with paper electrophoresis if the deceased patient had been afflicted with a carcinoma, a cirrhosis of the liver, an uncomplicated nephrosis, or with a paraproteinosis. Extensive métastases in a severely damaged liver (cirrhosis) did not lead to an increase ofα 2-globulin. Paraproteins could be detected by antigen analytical methods independent of the hemolytic condition of the cadaver serum. This was especially important when a paraprotein had not been revealed in the clinic.
    Notes: Zusammenfassung Aus Leichenseren ließen sich papierelektrophoretisch die Reaktions-Konstellationstypen 3, 5, 6 und 7 des Serumeiweißbildes nachWuhrmann undMärki bestimmen, wenn die Verstorbenen entsprechend an einem Carcinom, einer Lebercirrhose, einer unkomplizierten Nephrose oder einer Paraproteinose gelitten hatten. Ausgedehnte Metastasierung in eine stark vorgeschädigte Leber (Lebercirrhose) führte nicht zu einer α2-Globulinerhöhung. Paraproteine konnten unabhängig vom Zustand des Leichenserums hinsichtlich des Ausmaßes der Hämolyse antigenanalytisch erfaßt werden. Das war besonders dann bedeutsam, wenn in der Klinik ein Paraprotein nicht nachgewiesen worden war.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00959696
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  • 4
    Electronic Resource
    Electronic Resource
    Untersuchungen zum Verhalten der Eiweißkörper aus Leichenseren „in der Norm“ und bei Fällen mit akuter Entzündung und Nekrose (1965)
    Springer
    Virchows Archiv 339 (1965), S. 337-348 
    Details
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The behavior of the serum proteins of “normal” cadavers having the reaction constellation 1 does not exceed the range of variation of normal serum (from normal patients), if the serum is not hemolyzed, or if it is only slightly hemolyzed. With increasing hemolysis the hemoglobin becomes visible, especially with paper electrophoresis, as an extra gradient in the α2-β-globulin fraction. The hemoglobin adulterates the relative protein values of the individual fractions. By antigen analytic methods, we were not able to demonstrate changes of the serum proteins in cadaver serum which was obtainable up to 36 hours after death. One may regard the study of cadaver serum within the limits mentioned as an essential aid in pathologic-anatomic diagnoses.
    Notes: Zusammenfassung Das Verhalten der Serumeiweißkörper in Leichenseren „in der Norm“ und mit der Reaktions-Konstellation 1 überschreitet nicht den Variationsbereich von Normal- bzw. Patientenseren, wenn das zu untersuchende Serum nicht hämolytisch oder nur sehr leicht hämolytisch ist. Mit zunehmender Hämolyse macht sich vor allem in der Papierelektrophorese das Hämoglobin in Form eines Extragradienten imα 2-β-Globulinbereich als Störfaktor bemerkbar und führt zu einer Verfälschung der relativen Eiweißwerte der einzelnen Fraktionen. Antigenanalytisch war es uns nicht möglich, am Leichenserum, das bis 36 Std nach dem Tode gewonnen werden konnte, Veränderungen an den Serumeiweißkörpern nachzuweisen. Man kann die Untersuchung des Leichenserums innerhalb der abgesteckten Grenzen als eine wesentliche Bereicherung der pathologisch-anatomischen Diagnostik ansehen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00959695
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  • 5
    Electronic Resource
    Electronic Resource
    Fluorescence interference-contrast microscopy of cell adhesion on oxidized silicon (1997)
    Springer
    Applied physics 65 (1997), S. 341-348 
    Details
    ISSN: 1432-0630
    Keywords: PACS: 33.50.D; 68.35.G; 78.66; 87.22
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003390050589
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  • 6
    Electronic Resource
    Electronic Resource
    Augmentation of impaired tumoricidal function in alveolar macrophages from lung cancer patients by cocultivation with allogeneic, but not autologous lymphocytes (1997)
    Springer
    Cancer immunology immunotherapy 45 (1997), S. 29-36 
    Details
    ISSN: 1432-0851
    Keywords: Key words Alveolar macrophages ; Tumoricidal activity ; PBL ; Lung cancer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  It has been reported that the in vitro development of tumoricidal function in alveolar macrophages from lung cancer patients is reduced significantly when compared to that in peripheral blood monocytes from the same patients or alveolar macrophages from control patients. In the present investigation, a method for potentiating the development of tumoricidal function in alveolar macrophages from lung cancer patients is described. This method, which relies on priming the macrophages with purified, allogeneic peripheral blood lymphocytes from normal donors, could not be demonstrated when autologous lymphocytes from lung cancer patients were used in the priming coculture. The augmentation of tumoricidal function appears to be mediated by one or more soluble factors, since supernatants from cocultures of alveolar macrophages and allogeneic peripheral blood lymphocytes could enhance the cytotoxic function of freshly obtained alveolar macrophages. Furthermore, it appears that NK cells are necessary for this effect, since depletion of CD56+/CD57+ cells from allogeneic lymphocytes eliminated their capacity to enhance alveolar macrophage cytotoxic function. The augmentation of cytotoxic function elicited in alveolar macrophages by this method was not associated with changes in the secretion of tumor necrosis factor α, or interleukin 1β.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002620050397
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  • 7
    Electronic Resource
    Electronic Resource
    Modulation of tumoricidal function in alveolar macrophages from lung cancer patients by interleukin-6 (1997)
    Springer
    Cancer immunology immunotherapy 45 (1997), S. 37-44 
    Details
    ISSN: 1432-0851
    Keywords: Key words Alveolar macrophages ; Tumoricidal activity ; PBL ; Interleukin-6 ; Lung cancer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Previous studies have demonstrated that alveolar macrophages from lung cancer patients are impaired in their ability to develop tumoricidal function when stimulated by activators such as interferon γ + lipopolysaccharide. However, these same macrophages have been shown to develop significant tumoricidal function when precultured with macrophage-depleted allogeneic peripheral blood lymphocytes from normal donors, an effect that was lost by the elimination of natural killer cells from the allogeneic lymphocyte population. In the present study, the effect of each activation condition on the expression of mRNA for interleukin-1α (IL-1α), IL-1β, tumor necrosis factor α (TNFα) and IL-6 was determined using reverse transcription/polymerase chain reaction. The results show that the non-permissive activation condition is associated with the expression of mRNA for IL-6 while the permissive activation condition is not. Antibodies against IL-6 were subsequently shown to permit the development of tumoricidal function in alveolar macrophages stimulated with interferon γ + lipopolysaccharide while IL-6 protein was shown to inhibit the stimulatory action of allogeneic lymphocytes on the development of tumoricidal function in the same alveolar macrophages. Neither the permissive (i.e. allogeneic lymphocyte stimulation) nor the non-permissive (i.e. interferon γ + lipopolysaccharide) activation condition had any effect on the capacity of alveolar macrophages from lung cancer patients to express mRNA for IL-1α, IL-1β or TNFα. These results show that IL-6 can regulate the ability of alveolar macrophages from lung cancer patients to be stimulated by interferon γ + lipopolysaccharide to develop significant tumoricidal function. They also show that allogeneic lymphocytes have the capacity to down-regulate IL-6 mRNA synthesis by alveolar macrophages thereby permitting the development and/or expression of macrophage tumoricidal function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002620050398
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  • 8
    Electronic Resource
    Electronic Resource
    Analysis of early treatment of accidents at work and on the way to or from work with special consideration of traumatic-hemorrhagic shock (1999)
    Springer
    International archives of occupational and environmental health 72 (1999), S. M062 
    Details
    ISSN: 1432-1246
    Keywords: Key words Occupational accidents ; Traumatic-hemorrhagic shock ; Emergency treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The study investigates the quality of the management of occupational accidents and of accidents on the way to or from work. Special consideration is given to the prevention and effective early treatment of traumatic-hemorrhagic shock, which in serious accidents is often the pathophysiological cause of reversible or irreversible damage of organ systems, disability or death. In a series of 725 consecutive occupational accidents, accidents on the way to or from work and road accidents over a period of 2 years, and in 31 additional individual cases, the emergency treatment and further care of patients with incipient or advanced shock were examined. Assessment was based on protocols of initial emergency treatment and medical records of further hospital care. Specific attention was given to the development of shock-induced organ damage. When the emergency physician arrived at the site of the accident, of the 725 cases, 82 patients showed early signs of shock, 37 were in advanced shock, and 24 were dead. In accidents with early intervention of experienced medical personnel, securing of the airways, control of bleeding and sufficient substitution of volume and oxygen were usually done professionally, but analysis of the management of other cases showed delays and various shortcomings. In 25% of those who died, protracted circulatory shock due to insufficient volume substitution was the cause of death. Occupational rehabilitation failed in two thirds of the patients with advanced shock, but only in one third of the patients with incipient shock. The study demonstrates that immediate intervention by non-professionals in first aid, rapid arrival of the emergency care team, sufficient volume and oxygen substitution along with other emergency measures, and rapid transport to an appropriate trauma care facility, all improved the prognosis of the accident victims. Specific areas for improvement in the management of accident victims were found, especially in co-operation and co-ordination among non-professional first aid providers, occupational health and auxiliary personnel, and emergency medicine professionals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00014212
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  • 9
    Electronic Resource
    Electronic Resource
    Über Darstellung und Eigenschaften einiger cyclischer und polymerer Trithiokohlensäureester (1965)
    Springer
    Monatshefte für Chemie 96 (1965), S. 631-641 
    Details
    ISSN: 1434-4475
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Abstract In a direct one-step reaction of carbon disulfide with aliphatic or araliphatic dithiols in the presence of tertiary amines and lead(II)-ions cyclic or polymeric esters of trithiocarbonic acid, depending on the number of carbon atoms between the mercapto groups, can be obtained. The properties and spectra of the esters are reported. The poly-trithiocarbonates prepared are compared with the polymers made from thiophosgene and dithiols.
    Notes: Zusammenfassung Durch direkte Umsetzung von Schwefelkohlenstoff mit aliphatischen oder araliphatischen Dithiolen in Gegenwart von tertiären Aminen und Blei(II)-Ionen lassen sich in einem Reaktionsschritt je nach der Zahl der Kohlenstoffatome zwischen den Mercaptogruppen cyclische oder polymere Ester der Trithiokohlensäure erhalten. Es wird über deren Eigenschaften und Spektren berichtet. Die dargestellten Poly-trithiocarbonate werden mit den aus Thiophosgen und Dithiolen entstehenden Polymeren verglichen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00909481
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  • 10
    Electronic Resource
    Electronic Resource
    Versuche zur Darstellung von 4- Vinyltriarylmethylradikalen (1969)
    Springer
    Monatshefte für Chemie 100 (1969), S. 968-975 
    Details
    ISSN: 1434-4475
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Abstract The attempted preparation of 4-vinyltriarylmethyl radicals by interaction of 4-vinyltriarylmethylchlorides with metals yielded unstable radical intermediates immediately undergoing further reaction to give polymers of a quinodimethane structuretype. 1,4-α,α-Diphenylquinodimethane, obtained from 4-methyltriphenylmethyl chloride and pyridine in solution, was used as a model compound. It cannot be isolated and readily polymerizes to give poly-1,4-α,α-diphenylxylylene identified by IR and NMR spectra.
    Notes: Zusammenfassung Beim Versuch zur Darstellung von 4-Vinyltriarylmethylradikalen durch Umsetzung von 4-Vinyltriarylmethylchloriden mit Metallen entstehen intermediär Radikale, die jedoch sofort in einer mesomeren Form weiterreagieren, wobei Polymere mit Chinodimethan-Strukturen entstehen. Als Modell hierfür dient das gelbe 1,4-α,α-Diphenylchinodimethan, das aus 4-Methyltriphenylmethylchlorid und Pyridin in Lösung erhalten werden kann; es ist nicht in fester Form isolierbar und polymerisiert sich leicht zu Poly-1,4-α,α-diphenylxylylen, das durch IR- und NMR-Spektren identifiziert wird.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00900584
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