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Notes: Background We have recently reported that soluble ST2 protein levels are elevated in the sera of patients with asthma, and correlate well with the severity of asthma exacerbation. However, the role, function, and kinetics of soluble ST2 expression in asthma remain unclear.Objective The objective of the present study was to clarify the function and kinetics of soluble murine (m) ST2 expression in a murine asthma model.Methods We analyzed the kinetics of gene and protein expression of mST2 in sera or lung tissue after allergen (ovalbumin; OVA) challenge in a murine model of allergic airway inflammation, the effects of mST2 protein on OVA-induced Th2 cytokine production in vitro from splenocytes of sensitized mice, and the effects of soluble mST2 on Th2-dependent allergic airway inflammation by in vivo gene transfer of mST2.Results Serum mST2 protein levels increased to the maximal level 3 h after the allergen challenge, before serum IL-5 levels peaked. The mRNA expression of mST2 in lung tissue was induced after the allergen challenge, while that in the spleen was constitutively detected. Furthermore, pre-treatment with mST2 protein significantly inhibited the production of IL-4 and IL-5, but not IFN-γ, from OVA-stimulated splenocytes in vitro, and intravenous mST2 gene transfer resulted in a drastic reduction in the number of eosinophils and in the levels of IL-4 and IL-5 in bronchoalveolar lavage fluid, compared with those in response to transfer of non-coding plasmid vector or of lipid alone.Conclusion These results suggest that increases in endogenous mST2 protein after allergen exposure may modulate Th2-mediated airway inflammation, and that in vivo gene transfer of mST2 can be applicable to use in a novel immunotherapy for allergic diseases.

Notes: Summary 1. A preparation has been developed for the study of central auditory neurophysiology in cetaceans. Under N2O and Fluothane anesthesia, supplemented by succinylcholine chloride, twenty-nine specimens of four species of dolphins, most commonly Stenella caeruleo-alba, were stimulated with air-borne sound, water-borne sound, and directly by hydrophone pressed against the skin of the head. Evoked potential responses were recorded chiefly from the inferior colliculi but also from medullary auditory centers and the medial geniculate. 2. The recorded potentials were onset responses of complicated waveform, usually having components with latencies of 2–4 msec. The longer latency responses probably represent heterogenous input to the inferior colliculi and, in a few cases, activity of collicular cells or input to the medial geniculate body. Shorter latency responses probably represent output of the cochlear nuclei and activity of medullary auditory nuclei. 3. Although the evoked responses do not reflect increases in stimulus duration beyond a fraction of 1 msec, response threshold and waveform are sensitive to changes in rise time between 0.1 and 1.0 msec, and the small duration necessary to provide the onset response (as little as 0.1 msec) is also sufficient to provide accurate information about the frequency of the stimulus. 4. Evoked potential responses in certain locations and intensity ranges were found to change markedly in amplitude or waveform with intensity changes as small as 1 db. The same response in other intensity ranges might be little altered by intensity changes as large as 10 db. 5. Measurements of sensitivity as a function of frequency varied with electrode location, but averages or determinations of maximum sensitivity at each frequency at any location in a given animal produced audiograms in close agreement with that obtained behaviorally in Tursiops truncatus by Johnson (1966). Sensitivity was maximum at about 60 kc/s, and high between 20 and 70 kc/s; it typically fell about 25 db between 20 and 10 kc/s, and the same amount between 60 and 100 kc/s. The highest frequency eliciting a response was 120–140 kc/s. Data from the four species were not noticeably different. 6. Response waveform and amplitude were often changed much more dramatically than was threshold by small changes in stimulus frequency, emphasizing the heterogeneity of input to a given electrode location, and the sharpness of frequency discrimination. Changes in threshold of up to 3 db/percent change in frequency were seen. Actual frequency discrimination is undoubtedly much sharper than these evoked potential measurements would indicate. 7. The masking effect of background tones was shown to be confined to a relatively narrow band of frequencies surrounding that of the stimulus. Changes in masking effectiveness reached 30 db/10 kc/s change in background tone frequency. This too provides a conservative estimate of response “tuning”, since the evoked potentials represent the summed activity of such a large population of different units. 8. Frequency modulated (FM) pulses of 2–5 msec duration and gradual rise and fall times were found sometimes to produce unexpectedly large responses compared with the response to any constant frequency (CF) pulse within the swept range. The response waveforms differed sharply with changes in starting frequency or range swept, even when the maximum response amplitude remained unchanged. In certain cases an FM pulse was found to be up to 10 db more effective if swept upward than if swept downward, and vice versa. It seems probable that significant populations of cells exist that are specifically responsive to sounds having certain FM characteristics, or certain combinations of frequencies in a given temporal order. 9. Temporal resolution of successive sounds by cetaceans was found to be extremely rapid; virtually complete recovery of responsiveness in the fastest case took 0.8–1.0 msec between clicks; some initial recovery was often visible at approximately 0.5 msec. Following of a train of click stimuli was seen at repetition rates up to 2,000/sec. However, more typical recordings showed complete recovery of the collicular evoked potential required 3–5 msec. There was no evidence of facilitation of response to the second of two identical stimuli, as has been observed in echolocating bats. 10. Distortion of the sound field by holding a sheet of paper between the source and the lower jaw or melon can in some recording sites cause dramatic changes in response waveform without significantly affecting the maximum response amplitude. 11. The primary pathway of sound to the cochlea is via the lower jaw. When stimuli were applied either with a loudspeaker in air, a hydrophone pressed against various points on the head, or a hydrophone underwater, sensitivity was consistently greatest over a limited portion of the side of the contralateral lower jaw, nearly as high over the ipsilateral side of the melon, markedly lower over the side of the head including the external ear orifice, and almost nil at the rostrum. This map was confirmed by using a distant sound source and local acoustical shields of 10×12 cm pieces of foam rubber or paper; held over the sensitive part of the lower jaw or melon this caused marked attenuation of response. At low frequencies (30 kc/s) the same pattern was found, but sensitivity was somewhat more uniform, relatively greater over the side of the head including the external auditory meatus and reduced on the melon, compared with the high-frequency pattern (60 kc/s). 12. Gentle stroking of the side of the jaw or splashing of water on the jaw masked response to sounds. The effective input is acoustic. The effective area is circumscribed and identical to that for sound. Electrical stimulation of the skin of the jaw elicited no response in auditory centers and did not mask responses to sounds. 13. A cone of best reception is directed forward and downward, some 5-30° from the midline in the horizontal plane (the higher the frequency the closer to the midline), and 5–20° below the horizontal in the optimum vertical plane. Sensitivity fell sharply (up to 1.5 db/degree) at more lateral angles. Sound coming from above the horizontal is probably much reduced in effectiveness. 14. None of the response parameters studied was found to be affected by the anesthetics used, or by anesthetic doses of Nembutal. 15. The evoked response characteristics are evaluated from the standpoint of adaptations for echolocation, and compared with analagous responses in bats and non-echolocating mammals. Possible mechanisms of distance measurement are considered. Target localization is discussed in light of the patterns of angular sensitivity resulting from use of the lower jaws as primary pathways of sound to the ears. The question is raised: In view of the extreme sensitivity of the head surface to mechanical disturbance, especially around the lower jaw, is echolocation usable during natural activity, e.g. rapid swimming, pursuit of prey, or food mastication ? If so, what adaptations have occurred to help overcome the potential masking noise ?

Notes: Abstract We present an overview of the gene content and organization of the mitochondrial genome of Dictyostelium discoideum. The mitochondria genome consists of 55,564 bp with an A + T content of 72.6%. The identified genes include those for two ribosomal RNAs (rnl and rns), 18 tRNAs, ten subunits of the NADH dehydrogenase complex (nad1, 2, 3, 4, 4L, 5, 6, 7, 9 and 11), apocytochrome b (cytb), three subunits of the cytochrome oxidase (cox1/2 and 3), four subunits of the ATP synthase complex (atp1, 6, 8 and 9), 15 ribosomal proteins, and five other ORFs, excluding intronic ORFs. Notable features of D. discoideum mtDNA include the following. (1) All genes are encoded on the same strand of the DNA and a universal genetic code is used. (2) The cox1 gene has no termination codon and is fused to the downstream cox2 gene. The 13 genes for ribosomal proteins and four ORF genes form a cluster 15.4 kb long with several gene overlaps. (3) The number of tRNAs encoded in the genome is not sufficient to support the synthesis of mitochondrial protein. (4) In total, five group I introns reside in rnl and cox1/2, and three of those in cox1/2 contain four free-standing ORFs. We compare the genome to other sequenced mitochondrial genomes, particularly that of Acanthamoeba castellanii.

Notes: Abstract  Hydrothermal hot-pressing (HHP) technique was used to solidify calcium silicate hydrate powders at a relatively low temperature as 200°C and low pressure (20 MPa) with short reaction time (30 min). Diatomaceous earth, α-quartz, fly ash and silicic acid were used as the Si sources. Waste rice husk was used for fiber reinforcement to strength the hydrothermal hot pressed calcium silicate compacts. The tensile strength of the solidified bodies with and without rice husk was tested. The solidified bodies with reinforced by 5% rice husk exhibit high tensile strength (∼9 MPa), which is almost three times lager than their non-additives compacts. There is no effect of additives for the density of solidified bodies, which remains almost constant at ∼2gcm–3 similar to their non-additive compacts. The developed low temperature solidification method is expected to be useful in the development of an environmentally friendly processing route for making artificial wood.

Notes: Abstract Tracer experiments on the chemical transformation of technetium in paddy soil and the transfer to rice seedlings have been carried out using 95mTc as a tracer. Two common Japanese soils, Andosol and Gray lowland soil were used in the soil incubation experiments. The chemical form of soluble Tc in soil water was a mixture of Tc-organic matter complex, Tc-iron complex and pertechnetate. An uptake experiment with rice seedlings using nutrient solution showed that the Tc-organic matter complex was less available than pertechnetate or the Tc-iron complex. These chemical forms of Tc were also observed in the root bleeding sap of rice seedlings when introduced to the nutrient solution containing soluble Tc. These results suggested that the transfer of technetium from soil to rice would depend on the chemical form of Tc and they would transport from the root to the leaf without chemical transformation.

Notes: Abstract The stability of the lead scandium niobate (PSN) and itssolid solution with lead titanate (PSNT), both with the perovskitestructure, was investigated in a platinum capsule under hydrothermalconditions. The perovskite solid solution of PSNT decomposes in waterat 640°C, which results in the transformation to thetetragonal structure after the treatment. In KOH solutions, the PSNTpowder was partially dissolved, it results in the precipitation ofPb2ScNbO6 powders with the perovskite structure, and thegrowth of both lead oxide (massicot) and K4Ti3O8needle crystals at the top of the capsule. In contrast, singlecrystals of both PSNT or PSN were grown in KF solutions at the top ofthe capsule by hydrothermal treatments at 600°C with atemperature gradient of 40°C. In both cases a deficiency ofthe scandium content achieves the stabilization of the pyrochlorestructure of the grown crystals.