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  • 1960-1964  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Submerged Citric Acid Fermentation of Sugar Beet Molasses. Effect of Ferrocyanide Control (1962)
    s.l. : American Chemical Society
    Industrial and engineering chemistry 1 (1962), S. 59-62 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/i360001a013
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Submerged citric acid fermentation of ferrocyanide-treated cane molassesContribution from the Division of Applied Biology, National Research Council, Ottawa 2, Canada. Issued as N.R.C. No. 6722 (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 17-21 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Ferrocyanide-treated cane molasses was fermented in 2.5-l. Pyrex tower fermenters using pellets of Aspergillus niger, grown in beet molasses, as an inoculum. The success of the process depended on accurate control of the ferrocyanide concentration in the fermentation mash. Highest yields (up to 80% conversion of available sugar in 8 days) were obtained when the ferrocyanide concentration was 20 p.p.m. at the start of fermentation and then increased to 80-150 p.p.m. at the 24-hr. period. Addition of phosphate, nitrate, or methanol to fermentation mash did not increase rate of acid production or final yield.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040104
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Aeration in tower-type fermentors: Effect of tower diameter and number of aeration disksContribution No. 6866, from the Division of Applied Biology, National Research Council, Ottawa, Canada (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 241-245 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The rate of oxygen absorption by aqueous sodium sulphite solutions in tower-type fermentors up to 6 in. in diameter was related both to tower diameter and to the number of sintered Pyrex disks used for dispersing air entering the towers. Maximal rates of absorption were obtained in towers 2.5 in. in diameter. Under comparable conditions, rates of oxygen transfer in towers containing one aeration disk were approximately 30% higher than those in similar towers containing two or more disks. In towers greater than 6 in. in diameter, the absorption rate was independent of vessel diameter and number of aeration disks when more than one disk was used. Bubble coalescence was the main cause of the difference in the rate of oxygen absorption.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040214
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Uniform inoculation of nutrient surfacesContribution from the Division of Applied Biology, National Research Council, Ottawa, Canada. Issued as N.R.C. No. 7650. (1963)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 123-129 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method of inoculating nutrient surfaces uniformly with bacterial cells has been developed. The method is based on controlled exposure of samples in a humidified chamber to a spray produced from a bacterial suspension. Factors affecting uniformity of inoculation (ionic strength of suspension, force of spray, distance between spray nozzle and sample, and exposure time) were studied in detail. Under optimum condition using agar medium in Petri plates, differences between total counts for six plates inoculated simultaneously, or between counts for individual 2 cm.2 areas taken at any location on any of the plates, were not statistically significant at the 5% level. The coefficient of variability for counts on 2 cm.2 areas renged from 8 to 12% between tests of 216 counts each.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260050208
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Submerged citric acid fermentation of beet molasses in tank-type fermentersIssued as N.R.C. No. 7672. (1963)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 193-199 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Ferrocyanide-treated beet molasses was fermented in fully baffled mechanically agitated, 2.5-liter, tank-type fermenters using pellets of Aspergillus niger grown in shake flasks as an inoculum. The object of the study was to adapt the fermentation method previously developed in an unstirred tower-type fermenter to a conventional tank-type fermenter. Under selected conditions of stirring (agitator speed 400-700 rpm) and sparging (medium porosity sintered disk), high yields of citric acid (8.6% citric acid, 70% conversion of available sugar) were obtained in 140 hr. of fermentation. There was little relation between citric acid yield and aeration conditions in the fermenter as judged by mash viscosity measurements and sulfite oxidation studies.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260050304
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    Paper (German National Licenses)
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