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  • 1
    Electronic Resource
    Electronic Resource
    Expression of the human α-1-antitrypsin gene in transgenic rats (1995)
    Springer
    Bulletin of experimental biology and medicine 120 (1995), S. 727-729 
    Details
    ISSN: 1573-8221
    Keywords: α-1-antitrypsin ; transgenic animals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A genetic-engineering construction is developed to provide expression of the full-size cDNA of human α-1-antitrypsin controlled by the promoter elements of the murine metallothionein-1 gene. Transgenic animals were obtained by microinjection of pAT-MT plasmid into fertilized rat oocytes. The genome of such animals contains the sequences of human α-1-antitrypsin-cDNA revealed by blot- and dot-hybridization. Integrated human α-1-antitrypsin-cDNA copies are found with a high frequency (9 out of 22 animals) in the second-generation offspring obtained after crossings of transgenic animals. A protein with antigenic specificity of human a-1-antitrypsin is found in the serum of transgenic rats by the immunoblotting method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02444673
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of the human α-1-antitrypsin gene in heterologous mammalian cells (1994)
    Springer
    Bulletin of experimental biology and medicine 117 (1994), S. 166-167 
    Details
    ISSN: 1573-8221
    Keywords: α-1-antitrypsin ; cell cultures ; gene transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A genetic-engineering construction is developed containing the full-size cDNA of human α-1-antitrypsin, controlled by the promotor and enhancer elements from cytomegalovirus. It is shown that, after transfection with this recombinant DNA, it is properly expressed in heterologous animal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02444134
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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