ISSN:
1432-2307
Keywords:
Human thyroid
;
TSH receptor
;
125I-TSH
;
Autoradiography
;
TSH binding sites
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary We have developed a morphological method to portray TSH binding sites in intact tissue specimens. Frozen sections were incubated with125I-labelled TSH so as to localise binding sites by autoradiography. The proof of specificity was substantiated by: the competitive inhibition of125I-TSH-labelling with cold TSH, the lack of binding in non-target tissues and a lack of binding in TSH target tissues after incubation with125I-hCG or free125I. In applying this method to a total of 22 surgical specimens of thyroid, striking differences came to light in respect of the degree to which125I-TSH binding occurred in the various thyroid disorders. When compared with histologically normal tissue, labelling was generally decreased in toxic adenomas, non-functioning adenomas (cold nodules), and thyroids affected by Graves' disease, whereas non-toxic colloid goitre cases clearly exhibited denser binding. Medullary and anaplastic carcinomas exhibited no specific labelling whilst binding varied in the differentiated carcinomas between no effective binding or a level resembling that found in normal thyroid tissue.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00713424
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