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  • 1
    ISSN: 1432-1912
    Keywords: Key words 5-Hydroxytryptamine (5-HT ; serotonin) ; 5-HT1D receptors ; 5-HT1Dβ receptors ; Endothelial cells ; Cyclic AMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Pharmacological evidence has suggested the presence of 5-hydroxytryptamine (5-HT, serotonin), 5-HT1D receptors on endothelial cells but these receptors have never been identified unambiguously on this type of cells. We now report that human umbilical vein endothelial cells (HUVEC) express 5-HT1D receptors coupled to inhibition of cyclic AMP formation. 5-HT and the 5-HT1D receptor agonists 5-carboxamidotryptamine (5-CT) and sumatriptan were approximately equipotent at inhibiting forskolin-stimulated cyclic AMP accumulation in HUVEC (mean pEC50 7.6–8.2, maximal effect 30% inhibition). The 5-HT1A receptor agonist, 8-OH-DPAT was clearly less potent (pEC50 6.2) and less efficacious. The selective 5-HT1D receptor antagonist, GR127935 (1 nM) markedly inhibited the effect of 5-HT (apparent pKB 10.8). Reverse transcription-polymerase chain reaction analysis showed the mRNA for 5-HT1Dβ receptors to be expressed in HUVEC. These results demonstrate the presence of functional 5-HT1D receptors and the expression of 5-HT1Dβ receptor mRNA in HUVEC. They support the involvement of 5-HT1Dβ receptors in endothelial-mediated responses to 5-HT.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1912
    Keywords: 5-Hydroxytryptamine (5-HT, serotonin) ; 5-HT1D receptors ; 5-HT1Dβ receptors ; Endothelial cells ; Cyclic AMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Pharmacological evidence has suggested the presence of 5-hydroxytryptamine (5-HT, serotonin), 5-HT1D receptors on endothelial cells but these receptors have never been identified unamiguously on this type of cells. We now report that human umbilical vein endothelial cells (HUVEC) express 5-HT1D receptors coupled to inhibitiion of cyclic AMP formatin. 5-HT and the 5-HT1D receptor agaonists 5-carboxamidotryptamine (5-CT) and sumatriptan were approximately equipotent at inhibiting forskolin-stimulated cyclic AMP accumulation in HUVEC (mean pEC50 7.6–8.2, maximal effect 30% inhibition). The 5-HT1A receptor agonist, 8-OH-DPAT was clearly less potent (pEC50 6.2) and less efficacious. The selective 5-HT1D receptor antagonist, GR127935 (1 nM) markedly inhibited the effct pf 5-HT (apparent pKB 10.8). Reverse transcription-polymerase chain reaction analysis showed the mRNA for 5-HT1Dβ receptors to be expressed in HUVEC. These results demonstrate the presence of functional 5-HT1D receptors and the expression of 5-HT1Dβreceptor mRNA in HUVEC. They support he involvement of 5-HT1Dβ receptors in endothelial-mediated responses to 5-HT.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1434-601X
    Keywords: 36.10.Gv ; 13.75.Cs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The strong ¯p-nucleus spin-orbit interaction was investigated in a measurement of the strong-interaction effects of the 9→8 transition in ¯p 174Yb at the Low-Energy Antiproton Ring (LEAR) at CERN. This measurement was part of an experimental programme where, for the first time, the fine-structure components of the last observable X-ray transition in a ¯p atom, which carries information on the strong ¯p-nucleus interaction, were resolved and studied individually. The observed splitting ΔE exp=2408±26 eV consists of the electromagnetic fine-structure splitting ΔE FS=2350 eV and an additional splitting Δɛ=58±26 eV. In addition, one finds a significant difference in the level widths of Δ=195±59 eV with the larger value⇊=1216±41 eV for the lower fine-structure level. This experiment follows an earlier measurement on ¯p 138Ba, where the transition 8→7 is influenced by the strong interaction. In this case, however, the fine-structure components could not be resolved. The results for174Yb may be attributed to a spin-orbit (LS) term in the complex strong-interaction potential.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1434-601X
    Keywords: 36.10.Gv ; 13.75.Cs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The yields of the atomic 4→3 transitions in antiprotonic14N,16,17,18O,19F, and23Na were measured at the CERN antiproton facility, LEAR. From these, the widths Γup of the 4f levels were determined to be 136±19meV (14N); 603±22 meV (16O); 731±35 meV (17O); 795±23 meV (18O); 2.79±0.16 eV (19F); and 23.8±7.4 eV (23Na).
    Type of Medium: Electronic Resource
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