ZIB

Digitale Medien

Role of low ecdysteroid titre in acquisition of competence for pupal transformation in Bombyx mori

Nagata, H. ; Ohtaki, T. ; Sakurai, S.

Amsterdam : Elsevier

Journal of Insect Physiology 33 (1987), S. 657-662

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ISSN: 0022-1910

Schlagwort(e): 20-hydroxyecdysone ; Bombyx mori ; ecdysteroid titre ; gut purge ; penultimate instar ; pupation

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Biologie

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1016/0022-1910(87)90135-1

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Digitale Medien

Differential timing of pupal commitment in various tissues of the silkworm, Bombyx mori

Ohtaki, T. ; Yamanaka, F. ; Sakurai, S.

Amsterdam : Elsevier

Journal of Insect Physiology 32 (1986), S. 635-637+639-642

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ISSN: 0022-1910

Schlagwort(e): 20-hydroxyecdysone ; Bombyx mori ; Pupal commitment ; imaginal disc ; juvenile hormone

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Biologie

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1016/0022-1910(86)90093-4

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Digitale Medien

Establishment of strongly neutralizing monoclonal antibody to human interleukin-6 and its epitope analysis

Ida, N. ; Sakurai, S. ; Hosaka, T. ; [weitere]

Amsterdam : Elsevier

Biochemical and Biophysical Research Communications 165 (1989), S. 728-734

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ISSN: 0006-291X

Schlagwort(e): [abr] ABTS; 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid ; [abr] BSA; bovine serum albumin ; [abr] ELISA; enzyme-linked immunosorbent assay ; [abr] HPLC; high performance liquid chromatography ; [abr] HRP; horseradish peroxidase ; [abr] IL-6; interleukin-6 ; [abr] MTT; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ; [abr] PBS; phosphate buffered saline ; [abr] hrIL-6; human recombinant interleukin-6

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Biologie , Chemie und Pharmazie , Physik

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1016/S0006-291X(89)80027-0

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Digitale Medien

Establishment of strongly neutralizing monoclonal antibody to human interleukin-6 and its epitope analysis

Ida, N. ; Sakurai, S. ; Hosaka, T. ; [weitere]

Amsterdam : Elsevier

Biochemical and Biophysical Research Communications 165 (1989), S. 728-734

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ISSN: 0006-291X

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Biologie , Chemie und Pharmazie , Physik

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1016/S0006-291X(89)80027-0

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Digitale Medien

A novel member of the bombyxin gene family: structure and expression of bombyxin G1 gene, an insulin-related peptide gene of the silkmoth Bombyx mori (1998)

Yoshida, I. ; Moto, K. ; Sakurai, S. ; [weitere]

Springer

Development genes and evolution 208 (1998), S. 407-410

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ISSN: 1432-041X

Schlagwort(e): Key words Bombyxin ; Insulin ; Bombyx mori ; Multigene family ; Brain neurosecretory cell

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract Bombyxin G1 gene, a novel insulin-related peptide gene of the silkmoth Bombyx mori, has been identified. The G1 gene encodes a precursor peptide which shows 41–56% and 28% sequence identities with preprobombyxins previously characterized and human preproinsulin, respectively. The G1 gene forms a pair with bombyxin C2 gene with opposite transcriptional orientation in a bombyxin gene cluster. The bombyxin G1 mRNA in Bombyx brain was shown to locate in four pairs of medial neurosecretory cells.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s004270050197

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Gene transfer into insect brain and cell-specific expression of bombyxin gene (1999)

Moto, Ken’ichi ; Abdel Salam, Salah Eldin ; Sakurai, S. ; [weitere]

Springer

Development genes and evolution 209 (1999), S. 447-450

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ISSN: 1432-041X

Schlagwort(e): Key words Bombyxin ; Insulin ; Bombyx mori ; Electroporation ; Cell-specific expression

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract A transgene reporter consisting of the bombyxin gene promoter and the green fluorescent protein coding region was introduced into intact brains of the silkworm Bombyx mori by in vitro electroporation. After in vitro culture of the brains, the fluorescence derived from the introduced reporter gene was observed in all cases in eight neurosecretory cells that had previously been identified as bombyxin-producing cells (BPCs). Although the fluorescence was not always observed in all cells, it was specific to BPCs, indicating that the reporter was under the control of the bombyxin gene promoter in a BPC-specific manner. Electroporatical introduction of a reporter gene was therefore found to be a suitable method for analyzing cell-specific expression in intact tissues and to be substitute for germ-line transmission of reporters in the transgenic system. Application of this technique enables us to analyze the cell-specific expression of transgene reporters within a few days and treat more than several dozens of the reporters within 1 month, which is difficult to do with the transgenic system.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s004270050277

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