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  • 21.60.EV  (2)
  • DNA markers  (2)
  • 23.20.−g  (1)
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  • 1
    Digitale Medien
    Digitale Medien
    Detection of DNA sequence polymorphisms among wheat varieties (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 573-578 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Common wheat ; RAPD ; DGGE ; DNA polymorphism ; DNA markers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A DNA marker detection strategy that allows the rapid, efficient resolution of high levels of polymorphism among closely related lines of common wheat (Triticum aestivum) has been developed to circumvent the apparent lack of restriction fragment length polymorphism in many important self-pollinated crop species. The technique of randomly amplified polymorphic DNA (RAPD) was combined with a denaturing gradient gel electrophoresis system (DGGE) to explore DNA sequence polymorphisms among different genotypes of wheat. Of the 65 primer combinations used for the polymerase chain reaction (PCR) amplifications, over 38% of them produced readily detectable and reproducible DNA polymorphisms between a spring wheat line, SO852, and a winter wheat variety, ‘Clark’. A high level of polymorphism was observed among a number of commercial varieties and breeding lines of wheat. This procedure was also used to detect polymorphisms in a recombinant inbred population to test the feasibility of its application in genome mapping. This DNA polymorphism detection system provides an opportunity for pedigree analysis and fingerprinting of developed wheat lines as well as construction of a high density genetic map of wheat. Without the need for 32P and sophisticated DNA extraction procedures, this approach should make it feasible to utilize marker-based selection in a plant breeding program.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224154
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  • 2
    Digitale Medien
    Digitale Medien
    Association of a DNA marker with Hessian fly resistance gene H9 in wheat (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 964-968 
    Details
    ISSN: 1432-2242
    Schlagwort(e): RAPD ; Wheat ; Hessian fly ; DNA markers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The Hessian fly [Mayetiola destructor (Say)] is a major pest of wheat (Triticum aestivum L.) and genetic resistance has been used effectively over the past 30 years to protect wheat against serious damage by the fly. To-date, 25 Hessian fly resistance genes, designated H1 to H25, have been identified in wheat. With near-isogenic wheat lines differing for the presence of an individual Hessian fly resistance gene, in conjunction with random amplified polymorphic DNA (RAPD) analysis and denaturing gradient-gel electrophoresis (DGGE), we have identified a DNA marker associated with the H9 resistance gene. The H9 gene confers resistance against biotype L of the Hessian fly, the most virulent biotype. The RAPD marker cosegregates with resistance in a segregating F2 population, remains associated with H9 resistance in a number of different T. aestivum and T. durum L. genetic backgrounds, and is readily detected by either DGGE or DNA gel-blot hybridization.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224525
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  • 3
    Digitale Medien
    Digitale Medien
    Structure of theN=59 nucleus97Sr: coexistence of spherical and deformed states (1990)
    Springer
    The European physical journal 337 (1990), S. 149-159 
    Details
    ISSN: 1434-601X
    Schlagwort(e): 23.20.Lv ; 21.60.Ev ; 23.20.−g
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract A band with a rotational pattern based on a state at 585.1 keV has been identified in theN=59 neutron-rich nucleus97Sr. Its properties lead to the [422] 3/2 Nilsson-orbital assignment for the band head. There is evidence for a second band with the head at 644.7 keV and the configuration [541] 3/2. Since the ground state and the lowest excited levels are spherical, shape coexistence is established for97Sr. A deformed nature of several levels at 500–600 keV results also from QRPA-model calculations. The structure of the low-lying spherical levels has been studied in the frame of the IBF model. The results of the present investigations lead to a better understanding of theN=59 isotones which constitute the link between the spherical and deformed nuclei atA∼100 as a species with shape coexistence but without any indications of particular softness.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01294286
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  • 4
    Digitale Medien
    Digitale Medien
    The structure of the deformed nucleus 42 103 Mo61 (1993)
    Springer
    The European physical journal 346 (1993), S. 101-109 
    Details
    ISSN: 1434-601X
    Schlagwort(e): 21.60.EV ; 23.20.Ck ; 27.60.+j
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract The lifetimes of the three lowest excited members of the rotational ground-state band of103Mo at 102.6, 241.1 and 433.2 keV as well as of the levels at 353.8 and 456.1 keV have been determined through a measurement of the delayed coincidences betweenβ particles from the decay of the parent103Nb and theγ rays which depopulate the levels. A plastic and a BaF2 detector have been used for theβ andγ rays, respectively, in these experiments at the fission product separator JOSEF. The results for all three band members evince that this nucleus has a sizeable deformation, with a value ofβ q=0.34(1). This value is larger than that of the neighbouring even-even Mo isotopes which indicates an odd-even effect. On the other hand it confirms the observation that the Mo nuclei are in general less strongly deformed than their Sr and Zr isotones. Particle-rotor model calculations with the Nilsson parametersκ=0.084,μ=0.28 provide a good description of the ground-state band and of a side band based on the 346.5 keV level, including the new lifetimes. This substantiates the Nilsson orbital assignments of [411]3/2+ and [532]5/2− for these two bands, respectively, where the sideband is strongly affected by a [541]3/2− admixture. There is evidence for an additional band based on the 641.1 keV level for which the assignment [422]3/2+ is proposed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01294625
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  • 5
    Digitale Medien
    Digitale Medien
    Lifetime measurements and particle-rotor calculations for 42 105 Mo63 (1995)
    Springer
    The European physical journal 351 (1995), S. 13-18 
    Details
    ISSN: 1434-601X
    Schlagwort(e): 21.60.EV ; 23.20.Ck ; 27.60.+j
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract The half-lives of seven levels in105Mo have been determined with high precision at the fission-product separator Josef. Theβ-γ-γ triple coincidence method with plastic and BaF2 scintillators was used for a determination of the time between theβ q feeding of a level of interest and itsγ depopulation. A value ofβ q =0.38 (1) for the deformation parameter has been deduced from the half-lives of the first and second excited states. The results of Particle-Rotor model-calculations with the Nilsson parametersκ=0.084 andμ=0.28 support the assignment of the Nilsson orbital [532] 5/2− to the ground state of105Mo.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01292781
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