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  • 1
    Electronic Resource
    Electronic Resource
    5-Azacytidine-induced hypomethylation of tobacco HRS60 tandem DNA repeats in tissue culture (1991)
    Springer
    Planta 184 (1991), S. 487-490 
    Details
    ISSN: 1432-2048
    Keywords: 5-Azacytidine ; DNA methylation ; DNA repeat (tandem) ; Hypomethylation ; Nicotiana (hypomethylation of DNA) ; Tissue culture (DNA methylation)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The methylation status and 5-azacytidine-induced hypomethylation of CCGG sites within a family of tandemly organized, highly repeated DNA sequences of the Nicotiana tabacum L. nuclear genome (HRS60 family) were studied. As shown by in-situ hybridization experiments, the HRS60 family is clustered in a few regions of some tobacco chromosomes. The DNAs of leaf-derived calli, leaf-derived calli cultured on media with 5-azacytidine, and leaves were cleaved with restriction endonucleases differing in the sensitivity to the methylation of cytosine. After electrophoresis and Southern blotting they were hybridized with the HRS60 probe. We show that (i) CpG dinucleotides, and partially also CpCpG trinucleotides, of the HRS60 family are methylated in DNAs of the non-treated calli and leaves, and (ii) that these DNA repeats are sensitive to the action of a hypomethylating drug, 5-azacytidine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197896
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Maintenance of the induced hypomethylated state of tobacco nuclear repetitive DNA sequences in the course of protoplast and plant regeneration (1994)
    Springer
    Planta 194 (1994), S. 306-310 
    Details
    ISSN: 1432-2048
    Keywords: 5-Azacytidine ; DNA methylation ; DNA (tandem repeats) ; Nicotiana (tissue culture) ; Plant regeneration (tobacco)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Previously, we established experimental conditions allowing us to induce hypomethylation of tandem arrays of highly repetitive DNA sequences in the Nicotiana tabacum L. nuclear genome (M. Bezděk et al. 1991, Planta 184, 487–490). In this paper, we demonstrate that loci containing the highly repetitive sequences of the HRS60 family can maintain the induced hypomethylated state through protoplast regeneration, non-differentiated callus growth, and plant regeneration. The hypomethylation, induced with 5-azacytidine and monitored on these sequences, did not substantially alter the capacity of calli to produce plants. It can be concluded that, in contradistinction of multiple copies of transgenes or ectopic genes which are usually recognized as methylation targets, endogenous tandem repeats, such as the HRS60, present at 105 copies in the genome, can escape de-novo methylation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197529
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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